insertional mutagenesis
Insertional mutagenesis in yeasts using T-DNA from Agrobacterium tumefaciens
... Insertional mutagenesis is a powerful tool for the isolation of novel ...containing insertional mutations if there were no bias towards particular integration sites, as is the case in ...for ...
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A novel gammaretroviral shuttle vector insertional mutagenesis screen identifies SHARPIN as a breast cancer metastasis gene and prognostic biomarker
... novel mutagenesis screen using a replication-incompetent γRV shuttle vector to identify BC metastasis driver ...retroviral insertional mutagenesis screen to identify metastatic driver genes involved ...
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Cryptococcus neoformans Virulence Gene Discovery through Insertional Mutagenesis
... Insertional mutagenesis by biolistic transformation. Ap- proximately 4,300 nourseothricin-resistant transformants were obtained after biolistic transformation of a linear or circular plasmid into C. ...
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Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis
... relatively larger than those reported for M. oryzae and Arabidopsis, in which the majority of deletions ranged from 1 to 35 bp and 11 to 100 bp, respectively (Brunaud et al 2002,; Choi et al. 2007,; Forsbach et al. ...
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piggyBac-Based Insertional Mutagenesis and Enhancer Detection as a Tool for Functional Insect Genomics
... structs for insertional mutagenesis and enhancer detection To allow enhancer detectors to be directly employed used in the Drosophila pilot screen. The piggyBac-jumpstarter for misexpression studies, we ...
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Functional Analysis of the Dengue Virus Genome Using an Insertional Mutagenesis Screen
... transposon insertional mutagenesis, we overcame these challenges by simul- taneously screening a library of insertions at every position and determining tolerated positions by passaging the ...
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Insertional mutagenesis by the Tol2 transposon mediated enhancer trap approach generated mutations in two developmental genes: tcf7 and synembryn like
... Insertional mutagenesis by enhancer trapping In this study, we isolated two phenotypic mutants out of 54 enhancer trap ...retroviral insertional mutagenesis in which one in 80 insertions ...
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Retroviral Insertional Mutagenesis Can Contribute to Immortalization of Mature T Lymphocytes
... retroviral insertional mutagenesis can contribute to the immortalization of mature T ...sertional mutagenesis can alter the phe- notype of mature T lymphocytes, how- ever, the chance of ...
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Insertional Mutagenesis by a Hybrid PiggyBac and Sleeping Beauty Transposon in the Rat
... Transposon-mediated insertional mutagenesis is another approach that has been established in rodents, including the mouse and rat (Carlson et ...in insertional mutation, providing a molecular tag of ...
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Insertional Mutagenesis of Genes Required for Seed Development in Arabidopsis thaliana
... 1996 Insertional mutagenesis to dissect embry- genetic interference by double-stranded RNA in Arabidopsis thali- onic development in Arabidopsis, ...
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Insertional mutagenesis in transgenic mice generated by the pronuclear microinjection procedure
... We have also been able to use insertional mutagenesis as a powerful tool for generating mouse models for human disease. In addition to the skeletal defects in the TgN737Rpw transgenic line, homozygotes also ...
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Insertional mutagenesis combined with acquired somatic mutations causes leukemogenesis following gene therapy of SCID X1 patients
... X-linked SCID (SCID-X1) is amenable to correction by gene therapy using conventional gammaretroviral vectors. Here, we describe the occurrence of clonal T cell acute lymphoblastic leukemia (T-ALL) promoted by ...
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Characterization of a Chinese Hamster Ovary Cell Line Developed by Retroviral Insertional Mutagenesis That Is Resistant to Sindbis Virus Infection
... SV infects most vertebrate cell lines and causes apoptosis. Cells have different susceptibilities to SV infection and to SV- induced apoptosis depending on cell type and maturity both in vivo and in vitro. Cellular ...
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Insertional mutagenesis in Neurospora crassa: cloning and molecular analysis of the preg+ gene controlling the activity of the transcriptional activator NUC-1.
... of various genes by transformation. Insertional mu- tagenesis by transformation had been used to generate mutants in Aspergillus nidulans. We have applied this techniqu[r] ...
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Identification of ENA1 as a Virulence Gene of the Human Pathogenic Fungus Cryptococcus neoformans through Signature-Tagged Insertional Mutagenesis
... tional mutagenesis with DNA delivery by electroporation or biolistic transformation, combined with the ability to identify the affected gene by using the known inserted DNA sequence and the complete genome ...
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Structural domains of the avian erythroblastosis virus erbB protein required for fibroblast transformation: dissection by in-frame insertional mutagenesis.
... In-frame insertions in the extracellular domain of the v-erbB protein, between the transmembrane domain and the kinase region, and in the extreme C terminus of this polypeptide did not a[r] ...
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Candida albicans Mds3p, a Conserved Regulator of pH Responses and Virulence Identified Through Insertional Mutagenesis
... grew equally well, but the mds3 pmd1 double mutant The main limitation in our mutagenesis strategy stems grew poorly at pH 9. All strains grew comparably on from the requirement for mitotic recombination or gene ...
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Female gametophytic mutants of Arabidopsis thaliana identified in a gene trap insertional mutagenesis screen
... insertion mutagenesis to provide tools for forward and reverse genetic ...tional mutagenesis projects – using either transposons or T-DNA insertions – have been isolated and loci essential for male and ...
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Genetic Analysis of Bacillus subtilis spo Mutations Generated by Tn917-Mediated Insertional Mutagenesis
... Although the collection of spo mutations described here adds significantly to the total of known loci, and thus helps to define the set of genes whose products are required for spo[r] ...
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Identification of a novel gene, Vin-1, in murine leukemia virus-induced T-cell leukemias by provirus insertional mutagenesis.
... To determine whether the rearrangements detected with KpnI within the Vin-l region indeed reflected the integration of a provirus or one of its recombinant MCF-type proviruses and, if so[r] ...
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