interferon regulatory factor 3/7
Critical Role for Interferon Regulatory Factor 3 (IRF-3) and IRF-7 in Type I Interferon-Mediated Control of Murine Norovirus Replication
... transcription factor(s) remain unknown; a signif- icant decrease in IFN- ␣ 4 and IFN-  mRNA induction during MNV infection was not observed in macrophages lacking IRF-1 compared to wild-type controls ...
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Innate immune responses induced by lipopolysaccharide and lipoteichoic acid in primary goat mammary epithelial cells
... (PTGS2), interferon induced protein with tetratricopeptide repeats 3 (IFIT3), interferon regulatory factor 3 (IRF3), myeloid differentiation primary response 88 (MYD88), nuclear ...
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Deregulation of HDAC5 by Viral Interferon Regulatory Factor 3 Plays an Essential Role in Kaposi's Sarcoma Associated Herpesvirus Induced Lymphangiogenesis
... K aposi’s sarcoma-associated herpesvirus (KSHV) has been identified as an etiologic agent of multicentric Castleman’s disease (MCD), primary effusion lymphoma (PEL), and Kaposi’s sarcoma (KS) (1–3). KS is a ...
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Inhibition of Interferon Regulatory Factor 3 Activation by Paramyxovirus V Protein
... Involvement of MDA5 in induction of innate immunity in SeV infection has been suggested by a study of infection of MDA5-KO mice with SeV (9). It has been demonstrated that the V protein, especially the C-terminal Vu ...
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Role of Interferon Regulatory Factor 3 in Type I Interferon Responses in Rotavirus-Infected Dendritic Cells and Fibroblasts
... When IRF-3 expression was examined in RRV-treated mDCs, there was no detectable loss of expression over time in repeated experiments. This could be because only a small frac- tion of the cells expressed NSP1 and ...
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Interferon Regulatory Factor 3 Is a Cellular Partner of Rotavirus NSP1
... investigation. NSP1 has a calculated molecular weight of ap- proximately 54,000 and is the least conserved protein encoded by the rotavirus genome when NSP1s of different strains are compared (23, 34). The N terminus ...
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Identification of TRAIL as an Interferon Regulatory Factor 3 Transcriptional Target
... similar to electrophoretic mobility shift assays but use an en- zyme-linked immunosorbent assay-based format. We prepared nuclear extracts from HT-29 cells that were either untreated or SeV infected. Extracts were ...
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Activation of Interferon Regulatory Factor 3 Is Inhibited by the Influenza A Virus NS1 Protein
... At 7 h postinfection, cells transfected with an empty pCAGGS vector showed distinct nuclear localization of GFP–mIRF-3 in more than 90% of the fields examined ...(Fig. 3, ...(Fig. 3, ...
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Human Rhinovirus Attenuates the Type I Interferon Response by Disrupting Activation of Interferon Regulatory Factor 3
... 1, 3, 5, and 7 ...1, 3, and 5 hpi, respectively) were used in one-way ANOVA to identify host cell genes that were significantly differentially expressed upon ...1-, 3-, and 5-h mock-infected ...
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Differential Modification of Interferon Regulatory Factor 3 following Virus Particle Entry
... teins. Pellets were incubated with nuclear buffer (20 mM HEPES [pH 7.3], 25% glycerol, 420 mM sodium chloride, 1.5 mM magnesium chloride, 0.2 mM EDTA [pH 8.0], 50 mM sodium fluoride, 0.5 mM dithiothreitol, 1 mM ...
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Interferon Regulatory Factor 3 Attenuates Reovirus Myocarditis and Contributes to Viral Clearance
... Within the heart, cell types such as myocytes and fibroblasts respond differently to reovirus infection, based on basal ex- pression levels of components of the IFN signaling circuit (43) and additional undefined ...
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Pestivirus Npro Directly Interacts with Interferon Regulatory Factor 3 Monomer and Dimer
... T he hallmark of the innate immune response against viruses is the activation of type I alpha/beta interferon (IFN- ␣ /  ) sig- naling in immune cells (1, 2). IFN- ␣ /  synthesis is triggered by several of the ...
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Inhibition of Beta Interferon Induction by Severe Acute Respiratory Syndrome Coronavirus Suggests a Two-Step Model for Activation of Interferon Regulatory Factor 3
... 3 protein of reovi- ruses (28), which are double-stranded RNA-binding ...(4, 7), dominant-negative interference with IRF function by viral homologues (38, 43, 73), binding to IRF-3 (51), or ...
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Interferon Regulatory Factor 3 Is Required for Viral Induction of Beta Interferon in Primary Cardiac Myocyte Cultures
... IRF-3 overexpression can induce IFN-  in the absence of viral infection. IRF-3 overexpression induced IFN- in the absence of viral infection in both L929 cells and primary car- diac myocyte cultures (Fig. ...
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Soluble amyloid triggers a myeloid differentiation factor 88 and interferon regulatory factor 7 dependent neuronal type-1 interferon response in vitro
... and interferon gamma-inducible protein 16 (IFI-16) are all examples of TLR-independent activators of the type-1 IFN response ...p-STAT- 3 signalling in response to ...
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Regulation of DNA-Raised Immune Responses by Cotransfected Interferon Regulatory Factors
... Interferon regulatory factor 1 (IRF-1), IRF-3, and IRF-7 have been tested as genetic adjuvants for influenza virus hemagglutinin (HA) and nucleoprotein vaccine ...
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Cleavage of Interferon Regulatory Factor 7 by Enterovirus 71 3C Suppresses Cellular Responses
... TRIF, a key adaptor of the TLR3 pathway (6). To explore whether EV71 3C has an additional target(s), we examined a panel of com- ponents in the innate immune pathways (data not shown). Cells were transfected with GFP-3C ...
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Virus-Activated Interferon Regulatory Factor 7 Upregulates Expression of the Interferon-Regulated BST2 Gene Independently of Interferon Signaling
... these results indicate that the presence of a single IRF binding site in the BST2 promoter is sufficient to elicit activation by type I IFN. IRF proteins can induce BST-2 upregulation in the absence of type I IFN ...
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The Infected Cell Protein 0 Encoded by Bovine Herpesvirus 1 (bICP0) Associates with Interferon Regulatory Factor 7 and Consequently Inhibits Beta Interferon Promoter Activity
... sary for activating productive infection. A single cysteine-to- glycine mutation in the zinc RING finger of bICP0 reduces the growth potential and virulence of BHV-1 in infected calves (30), suggesting that this domain ...
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3C Protease of Enterovirus D68 Inhibits Cellular Defense Mediated by Interferon Regulatory Factor 7
... 3C pro cleaves IRF7 at Gln-167 and Gln-189. Since IRF7 cleav- age produced two 20-kDa fragments representing the N terminus and one 40-kDa fragment representing the C terminus (Fig. 5A), we inferred that the IRF7 ...
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