interferon regulatory factor 4
Interferon Regulatory Factor 4 Is Involved in Epstein-Barr Virus-Mediated Transformation of Human B Lymphocytes
... processes. Interferon regulatory factor 4 (IRF-4) is a transcrip- tion factor and has oncogenic ...critical factor in EBV transformation and a useful target in the therapy ...
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Kaposi's Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor 4 (vIRF4) Perturbs the G1-S Cell Cycle Progression via Deregulation of the cyclin D1 Gene
... viral interferon regulatory factor 4 (vIRF4) ro- bustly downregulated c-Myc expression, generating a favorable environment for viral lytic replication ...
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Interferon Regulatory Factor 4 Is Activated through c-Src-Mediated Tyrosine Phosphorylation in Virus-Transformed Cells
... The importance of the oncogenic transcription factor interferon regulatory factor 4 (IRF4) in hematological malignancies has been increasingly recognized. We have previously identified ...
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Interferon regulatory factor 4 attenuates Notch signaling to suppress the development of chronic lymphocytic leukemia
... Molecular pathogenesis of Chronic Lymphocytic Leukemia (CLL) is not fully elucidated. Genome wide association studies have linked Interferon Regulatory Factor 4 (IRF4) to the development of ...
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Cooperation between Viral Interferon Regulatory Factor 4 and RTA To Activate a Subset of Kaposi's Sarcoma-Associated Herpesvirus Lytic Promoters
... viral interferon (IFN) regulatory factors (vIRFs), which are inserted between open reading frames (ORFs) 57 and 58 but transcribed in the opposite orientation (reviewed in reference ...
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Kv1.3 channel blockade with the Vm24 scorpion toxin attenuates the CD4+ effector memory T cell response to TCR stimulation
... of interferon regulatory factor 4 (IRF4) and heat shock protein 90 (Hsp90), as their expression augmented in a TCR-dependent activation context, and when T cells were activated with OKT3 in ...
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Inhibition of Beta Interferon Induction by Severe Acute Respiratory Syndrome Coronavirus Suggests a Two-Step Model for Activation of Interferon Regulatory Factor 3
... IRF-3 hyperphosphorylation assay. Cells were lysed in radioimmunoprecipi- tation assay (RIPA) buffer (50 mM Tris, pH 7.6, 150 mM NaCl, 1% NP-40) containing protease inhibitors (Complete protease inhibitor; Roche) and ...
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Interferon regulatory factor 8/interferon consensus sequence binding protein is a critical transcription factor for the physiological phenotype of microglia
... Mice were perfusion-fixed with 4% (w/v) paraformalde- hyde in PBS, and tissues were processed as reported pre- viously [20]. Tissue sections (6 μm-thick) were prepared by a cryostat (CM1950, Leica Microsystems, ...
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Interferon Regulatory Factor 1 and Type I Interferon Cooperate To Control Acute Gammaherpesvirus Infection
... FIG 4 IRF-1 and type I IFN receptor cooperate to induce type I or type II IFN-dependent antiviral state in primary macrophages. Primary macrophages were derived from bone marrow of mice of the indicated genotypes. ...
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Kaposi’s Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor
... Antisense K9 expression in BCBL-1 cells. Since K9 was read- ily detected in BCBL-1 cells by immunoblot analysis, we ex- amined the effect of K9 on IFN-regulated gene expression in these cells. We constructed a ...
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Inhibition of p53 Tumor Suppressor by Viral Interferon Regulatory Factor
... with 4% paraformaldehyde for 15 min, permeabilized with 70% ethanol for 15 min, blocked with 10% goat serum in PBS for 30 min, and reacted with 1:100-diluted primary antibody in PBS for 30 min at room ...
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Virus-Activated Interferon Regulatory Factor 7 Upregulates Expression of the Interferon-Regulated BST2 Gene Independently of Interferon Signaling
... V5V cells unresponsive to IFN- ␣ -induced upregulation of surface BST-2 (Fig. 7A, bottom panel). IRF1, IRF3c, or IRF7c was tran- siently coexpressed with green fluorescent protein (GFP) in HEK293T or HEK293T-V5V cells ...
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Interferon Regulatory Factor 7 Is Associated with Epstein-Barr Virus-Transformed Central Nervous System Lymphoma and Has Oncogenic Properties
... IRF-7 is associated with LMP-1-expressing CNS lymphoma cells. Finally, we used another assay to detect the relationship between LMP-1 and IRF-7 in CNS lymphomas, namely, dou- ble immunolabeling followed by confocal ...
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Identification of TRAIL as an Interferon Regulatory Factor 3 Transcriptional Target
... SeV infection leads to transcriptional upregulation of TRAIL. Heylbroeck et al. (10) and Weaver et al. (28) have implicated caspase 8 as a downstream effector in SeV-induced apoptosis. To identify the SeV-induced ...
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The Infected Cell Protein 0 Encoded by Bovine Herpesvirus 1 (bICP0) Associates with Interferon Regulatory Factor 7 and Consequently Inhibits Beta Interferon Promoter Activity
... A recent study demonstrated that a bICP0 zinc RING finger point mutant virus grows poorly compared to the rescued virus when cells are pretreated with imiquimod (30), a known acti- FIG. 4. bICP0 deletion mutant ...
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Interferon Regulatory Factor 3 Is a Cellular Partner of Rotavirus NSP1
... human interferon (IFN) regulatory factor 3 ...transcription factor that responds to virus infection by inducing the expres- sion of the genes for IFN-␣ and IFN- (4, 21, 26, ...
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Interferon regulatory factor-7 modulates experimental autoimmune encephalomyelitis in mice
... Fluorescence microscopy was used to localize infiltrating cells in the CNS. CD3+ T cells were increased in num- ber and more diffusely dispersed in white matter in the spinal cord of IRF7-deficient mice with EAE, ...
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<p>Transcription factor E2F1 positively regulates interferon regulatory factor 5 expression in non-small cell lung cancer</p>
... Total protein from frozen tissues or cells was extracted using a Total Protein Extraction Kit ((Beyotime). Protein concentrations were measured using the BCA Protein Assay Kit (Beyotime). Equal amount of protein was run ...
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Deregulation of HDAC5 by Viral Interferon Regulatory Factor 3 Plays an Essential Role in Kaposi's Sarcoma Associated Herpesvirus Induced Lymphangiogenesis
... endothelial lineage. First, KS seldom develops in organs lacking a lymphatic system (29–31). Second, LECs are more susceptible to KSHV infection than BECs (8). Third, KS-SCs mainly express LEC markers, as opposed to BEC ...
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HIV-1 Vpu Does Not Degrade Interferon Regulatory Factor 3
... and viral antigen expression, the cells were lysed in mammalian protein extraction reagent (Thermo Scientific) 2 days posttrans- fection, and cell lysates were separated in 4-to-12% bis-Tris gels (Invitrogen). ...
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