Interferon regulatory factor-5 (IRF-5)
Interferon regulatory factor 5 genetic variants are associated with cardiovascular disease in patients with rheumatoid arthritis
... Introduction: Rheumatoid arthritis (RA) is a complex polygenic inflammatory disease associated with accelerated atherosclerosis and increased cardiovascular (CV) disease risk. Interferon regulatory ...
6
<p>Transcription factor E2F1 positively regulates interferon regulatory factor 5 expression in non-small cell lung cancer</p>
... IRF5, Interferon regulatory factor 5; E2F1, E2F transcrip- tion factor 1; NSCLC, Non-small cell lung cancer; LC, lung cancer; IRF, interferon regulatory factors; ...
9
An Oligodeoxynucleotide with AAAG Repeats Significantly Attenuates Burn-induced Systemic inflammatory Responses by inhibiting interferon Regulatory Factor 5 Pathway
... necrosis factor-α (TNF-α) in the injured ...the interferon regulatory factor 5 (IRF5) binding site in the cis-regulatory elements of proinflammatory cytokines, we tried to ...
11
Interferon-Regulatory Factor 5-Dependent Signaling Restricts Orthobunyavirus Dissemination to the Central Nervous System
... of IRF-5 on antibody responses after OROV ...that IRF-5 might be required for optimal adaptive immunity against ...of IRF-5 in the humoral re- sponse against OROV, we determined ...
17
Loss of interferon regulatory factor 5 (IRF5) expression in human ductal carcinoma correlates with disease stage and contributes to metastasis
... H&E sections of formalin-fixed paraffin-embedded (FFPE) archival tissue specimens were reviewed by two patholo- gists (MH and NM) for histological evaluation of disease and grade. Slides from 19 patients with ADH, 24 ...
14
Interferon Regulatory Factor 5-Dependent Immune Responses in the Draining Lymph Node Protect against West Nile Virus Infection
... This activity modulates the early antiviral response and the tran- sition to adaptive immunity, especially in the B cell compartment. Our experiments suggest that in vivo, IRF5 does not have a major nonredundant role in ...
15
Interferon Regulatory Factor 5 Represses Expression of the Epstein-Barr Virus Oncoprotein LMP1: Braking of the IRF7/LMP1 Regulatory Circuit
... Regulation of LMP1 transcription is critical because it is FIG. 4. Repression of IRF5 function increases LMP1 protein levels in type II cells. (A) Overexpression of an IRF5 dominant-negative mutant elevates LMP1 levels ...
6
IRF5 governs liver macrophage activation that promotes hepatic fibrosis in mice and humans
... for 5 minutes at 40 g; the resulting pellet composed the hepatocyte fraction, and the supernatant was composed of ...for 5 minutes at 500 g; supernatant was removed, and pellet was snap-frozen in liquid ...
23
Development of Monoclonal Antibodies Against Human IRF 5 and Their Use in Identifying the Binding of IRF 5 to Nuclear Import Proteins Karyopherin α1 and β1
... identifying interferon-regulatory factor 1 (IRF-1), nine additional IRF family proteins have been ...identified. IRF-1 binds to elements in the gene promoter that encodes for ...
9
Differential Regulation of Interferon Regulatory Factor (IRF)-7 and IRF-9 Gene Expression in the Central Nervous System during Viral Infection
... of IRF-2 (33), IRF-3 (5), and IRF-9 (14) genes by a wide variety of cell types, while IRF-5 (10) and IRF-7 (6) genes are expressed predominantly by he- matopoietic ...the ...
14
Interferon-Regulated Pathways That Control Hepatitis B Virus Replication in Transgenic Mice
... the interferon (IFN)-inducible genes, those for the IFN regulatory factor 1 (IRF-1), RNase L, and double- stranded RNA-activated protein kinase (PKR) systems are some of the best ...
5
Inhibition of Interferon Regulatory Factor 7 (IRF7)-Mediated Interferon Signal Transduction by the Kaposi's Sarcoma-Associated Herpesvirus Viral IRF Homolog vIRF3
... and 5 ⬘ -TCT AGG AGG TCC TCA TCC CAA GCA- 3⬘), IFNa4 (5⬘-GAG GGC CTT GAT ACT CCT GGC ACA-3⬘ and 5⬘-TCT AGG AGG CTC TGT TCC CAA GCA-3 ⬘ ), IFNa6 (5 ⬘ -AGA TTT CCC CAG GAG GAG TTT GAT-3⬘ and ...
11
Interferon Regulatory Factor IRF-7 Induces the Antiviral Alpha Interferon Response and Protects against Lethal West Nile Virus Infection
... of IRF-7 in regulating host defense responses, we infected wild- type and IRF-7 ⫺/⫺ bone marrow-derived CD11c ⫹ mDC with WNV and assayed viral replication and IFN production ...in IRF-7 ⫺/⫺ mDC than ...
11
Respiratory Syncytial Virus Infection of Human Alveolar Epithelial Cells Enhances Interferon Regulatory Factor 1 and Interleukin-1β-Converting Enzyme Gene Expression but Does Not Cause Apoptosis
... sulfate, 5% 2-mercaptoetha- nol, 10% glycerol), heated at 95°C for 5 min, and electropho- resed on a sodium dodecyl ...and 5% skim milk and then treated with anti-ICE p20 rabbit antibody (Upstate ...
5
Type I interferonopathies in pediatric rheumatology
... IRF: interferon regulatory factors; ISG15: interferon-stimulated gene 15; JAK: Janus kinase; JASL: Japanese autoinflammatory syndrome with lipodystrophy; JMP: joint contractures, muscle ...
12
Chimeric oncogenic interferon regulatory factor 2 (IRF 2): Degradation products are biologically active
... fied IRF-2 ORF, 2 µl pGEMT easy vector (25 ng/µl), 1 µl T4 DNA Ligase (3U/µl), 5 µl ligation buffer (2X) (30 mM Tris-HCl pH ...ATP, 5% polyethylene ...in 5 ml of 100 µl/ml ampicillin ...
9
Interferon regulatory factor (IRF) 3 is critical for the development of experimental autoimmune encephalomyelitis
... the back. Mice were sacrificed after 9 to 12 days, and their lymph nodes and spleens were retrieved. Cells were cul- tured (spleens and lymph nodes combined) at a density of 8 × 10 6 cells/ml in RPMI 1640 medium with 10% ...
7
Critical Role for Interferon Regulatory Factor 3 (IRF-3) and IRF-7 in Type I Interferon-Mediated Control of Murine Norovirus Replication
... Elevated interferon (IFN) levels are observed in ex vivo-stimulated peripheral blood mononuclear cells and in serum from human volunteers and gnotobiotic pigs inoculated with HuNoV (39, ...protein 5 ...
9
Suppression of Growth by All-trans Retinoic Acid Requires Prolonged Induction of Interferon Regulatory Factor 1 in Cervical Squamous Carcinoma (SiHa) Cells
... RNA isolation and semiquantitative reverse transcription-PCR. Cells were washed with PBS and directly lysed in TriReagent-LS (Molecular Research Center, Inc., Cincinnati, Ohio) and precipitated according to the ...
5
Human Herpesvirus 8 Encodes an Interferon Regulatory Factor (IRF) Homolog That Represses IRF-1-Mediated Transcription
... the interferon (IFN) regulatory factor (IRF) family of transcription factors and has thus been named viral IRF (vIRF) (25, ...human IRF family proteins, much of its homology is ...
7