internal transcribed spacer 2
Genotoxic Evaluation Of Insecticides On Internal Transcribed Spacer 2 (Its 2) Dna In Culex Quinquefasciatus By Polymerase Chain Reaction Technique
... dose level on a mosquito Culex quinquefasciatus taken as an experimental model. The selected insecticides are imidacloprid and acetamirpid which are the novel class of insecticides and belongs to newer class of ...
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Rapid Molecular Identification of Pathogenic Yeasts by Pyrosequencing Analysis of 35 Nucleotides of Internal Transcribed Spacer 2
... Rapid identification of yeast species isolates from clinical samples is particularly important given their innately variable antifungal susceptibility profiles. Here, we have evaluated the utility of pyrosequencing ...
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Identification of Leishmania spp by Molecular Amplification and DNA Sequencing Analysis of a Fragment of rRNA Internal Transcribed Spacer 2
... Isoenzyme analysis of cultured parasites is the conventional approach for Leishmania species identification. Molecular approaches have the potential to be more sensitive and rapid. We designed PCR generic primers to ...
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Functional constraints and evolutionary dynamics of the repeats in the rDNA internal transcribed spacer 2 of members of the Anopheles barbirostris group
... Long ITS2 regions have been found in distant taxa in- cluding oomycetes [34] and amphipods [35]. They have also been reported in several arthropod species, includ- ing rhipicephaline ticks [23] and insects of various or- ...
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Assessment of Ribosomal Large Subunit D1 D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
... Table 2 shows the results of pairwise nucleotide sequence analysis of the D1-D2 region, and Tables 3 and 4 show the same analysis but for the ITS1 and ITS2 ribosomal ...
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Occurrence and genetic variability of Phlebotomus papatasi in an urban area of southern Italy
... Background: A phlebotomine sand fly was noticed in the second floor of an old building in a highly urbanized area of southern Italy. A short-term entomological survey was carried out in the subsequent weeks to this ...
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Rapid Differentiation of Aspergillus Species from Other Medically Important Opportunistic Molds and Yeasts by PCR Enzyme Immunoassay
... the internal transcribed spacer 2 region of ribosomal DNA from Aspergillus flavus, Aspergillus fumigatus, Aspergillus nidulans, Aspergillus niger, Aspergillus terreus, Aspergillus ustus, and ...
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Surveillance of Helminthes and Molecular Phylogeny of Fasciola Gigantica Infecting Goats in Sadat District, Egypt
... and Internal Transcribed Spacer 2 (ITS2) of the ribosomal RNA gene were used to identify Fasciola species that infects cattle in Qena province, Upper Egypt, and they concluded that, to ...
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Rapid Identification of Candida dubliniensis Using a Species Specific Molecular Beacon
... the internal transcribed spacer 2 (ITS2) region of rRNA genes from reference Candida strains was used to develop molecular beacon probes for rapid, high-fidelity identification of ...
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Internal Transcribed Spacer Regions of rRNA Genes of Pneumocystis carinii from Monkeys
... To determine whether the sequences of the ITS regions were variable among different P. carinii f. sp. macacae isolates, 13 additional P. carinii-infected monkey lung specimens were examined. All 13 specimens generated ...
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Identification of Aspergillus Species Using Internal Transcribed Spacer Regions 1 and 2
... and 2 sequence analysis can be used for recognition of many fungal genera, including those that do not typically cause invasive disease such as airborne allergenic ...
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Phylogenetic Analysis of The Internal Transcribed Spacer (ITS) Region in Laccase Producing Pseudomonas aeruginosa ADN04 by Predicted rRNA Secondary Structure
... includes 2 fungus (F3 and F7), 7 bacteria (B10, B13, B8, B5, B3, B1, B2), 8 insects (I1, I2, I3, I4, I5, I6, I7, I8), 3 plants (P1, P9, P3) and Pseudomonas aeruginosa ADN04 come under clade ...clade 2 was ...
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Identification of Clinical Staphylococcal Isolates from Humans by Internal Transcribed Spacer PCR
... identification. Internal transcribed spacer-PCR (ITS-PCR) was used to identify a collection of 617 clinical staphylococcal ...simulans, 2 of ...
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Multiplex PCR Using Internal Transcribed Spacer 1 and 2 Regions for Rapid Detection and Identification of Yeast Strains
... Multiplex PCR amplification followed by either agarose gel electrophoresis (PCR-AGE) or microchip electrophoresis (PCR-ME) was used to test a total of 120 fungal strains. The internal transcribed ...
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rRNA Gene Internal Transcribed Spacer 1 and 2 Sequences of Asexual, Anthropophilic Dermatophytes Related toTrichophyton rubrum
... In molecular studies of recently evolved species complexes, e.g., the study of Lake Victoria cichlid fish (24) and the study of fungal crop pathogens in the genus Sclerotinia (3), it has been found that only the most ...
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Phylogenetic Classification and Species Identification of Dermatophyte Strains Based on DNA Sequences of Nuclear Ribosomal Internal Transcribed Spacer 1 Regions
... In addition to establishing the significance of the ITS1 re- gion from a taxonomic standpoint, we also identified these clinically important species using the ITS1 DNA sequence database. With this system, not only the ...
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Imported cutaneous leishmaniasis: molecular investigation unveils Leishmania major in Bangladesh
... A TaqMan probe based real-time (RT)-PCR assay, which targets the conserved region of Leishmania REPL repeats (L42486.1) of L. donovani complex, was carried out [5]. A threshold cycle (Cq) of 40- in a 45-cycle assay was ...
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Genotypic Study of Pneumocystis jirovecii in Human Immunodeficiency Virus Positive Patients in Thailand
... Pneumocystis jirovecii is one of the common opportunistic infections in human immunodeficiency virus (HIV)-infected patients in Thailand. Information regarding genotypic and epidemiological of this organism in Thai ...
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PCR Analyses of tRNA Intergenic Spacer, 16S 23S Internal Transcribed Spacer, and Randomly Amplified Polymorphic DNA Reveal Inter and Intraspecific Relationships ofEnterobacter cloacae Strains
... The most striking data presented in this study were that the use of tDNA-PCR and ITS-PCR together offered a hierarchi- cal system for E. cloacae strain differentiation; tDNA-PCR resolved the cultures at the species level ...
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Typing of Histoplasma capsulatumIsolates Based on Nucleotide Sequence Variation in the Internal Transcribed Spacer Regions of rRNA Genes
... FIG. 2. Sequences of ITS1, 5.8S rRNA gene, and ITS2 of H. capsulatum. U18363 is the A. capsulatus sequence deposited in the GenBank database. Sequence #16 (positions 101 to 596) is the first H. capsulatum sequence ...
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