Intracellular cytokine
Comparison of Intracellular Cytokine Flow Cytometry and an Enzyme Immunoassay for Evaluation of Cellular Immune Response to Active Tuberculosis
... whole-blood cytokine production in vitro by two methods after stimulation with CFP-10 and ESAT-6 in a group of patients with suspected active ...single-cell intracellular cytokine flow cytometry ...
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ART implantation failure and miscarriage in patients with elevated intracellular cytokine ratios: response to immune support therapy
... CD4+ intracellular cytokine ratios (CKR) prior to their next ART cycle using the investigative technique as previously described ...detect intracellular cyto- kines, monoclonal antibodies to TNF- α , ...
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Rorγt+ innate lymphocytes and γδ T cells initiate psoriasiform plaque formation in mice
... Intracellular cytokine staining of γδ subpopulations in the skin at peak disease revealed that Vγ4+ cells produced IL-17F and IL-22, whereas DETCs did not Figure 2C, suggesting that Vγ4+[r] ...
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Immunological diagnosis as an adjunctive tool for an early diagnosis of tuberculous meningitis of an immune competent child in a low tuberculosis endemic country: a case report
... an intracellular cytokine flow cytometry (ICCFC) protocol to asses mono-functional and multi- functional Mtb-specific CD4+ in peripheral blood and we proposed an immune-based approach, which could improve ...
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Anti-oxidant mediated normalisation of raised intracellular cytokines in patients with reproductive failure
... a cytokine assessment over the study period, and 187 cases (55%) had no evidence of raised intracellular cytokine ratios, with levels either within (n = 147) or below (n = 37) the reported normal ...
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Measurement of Cytokine Secretion, Intracellular Protein Expression, and mRNA in Resting and Stimulated Peripheral Blood Mononuclear Cells
... ulated cells compared to the unstimulated controls. At the 24-h time point, IL-2 message was 10-fold increased in the dually stimulated cells compared to controls, IL-4 was induced 1.5- fold over the unstimulated ...
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Robust NK Cell-Mediated Human Immunodeficiency Virus (HIV)-Specific Antibody-Dependent Responses in HIV-Infected Subjects
... standard intracellular cytokine assays on fresh whole blood from a cohort of 26 HIV-infected subjects identified non-T lymphocytes expressing gamma interferon (IFN- ␥ ) in response to overlapping linear ...
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Differential Modulation of Surface and Intracellular Protein Expression by T Cells after Stimulation in the Presence of Monensin or Brefeldin A
... and intracellular staining for cytokines was performed via a slightly modified version of the protocol supplied by BD Pharmingen (supplied in their intracellular cytokine staining ...For ...
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Target organ localization of memory CD4+ T cells in patients with chronic beryllium disease
... Using intracellular cytokine staining as a measure of response, BAL T cells were stimulated with beryllium or SEB for a total of 6 ...of intracellular cytokine stain- ing of BAL cells from a ...
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Detailed characterization of human Mycobacterium tuberculosis specific HLA-E restricted CD8(+) T cells
... with intracellular (cytokine) staining and that allowed us to describe for the first time the characteristics of these unconventional, HLA-E restricted Mtb-specific CD8 + T cells in cohorts of active TB ...
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Determination of antigen specific memory/effector CD4+ T cell frequencies by flow cytometry: evidence for a novel, antigen specific homeostatic mechanism in HIV associated immunodeficiency
... “TH2” cytokine syn- thesis phenotype might qualitatively impair effector responses to microbial Ags, and thereby contribute to immune deteriora- tion in AIDS (7, 8, ...their cytokine synthesis ...
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Blood feeding by the Rocky Mountain spotted fever vector, Dermacentor andersoni, induces interleukin 4 expression by cognate antigen responding CD4+ T cells
... glands were removed and placed together into a minimal volume of 0.15 M Dulbecco's phosphate buffered saline, pH 7.2 (Gibco) held on ice. Salivary glands from males and females were pooled separately and sonicated at 55 ...
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Nanoparticulate <em>Quillaja saponin</em> induces apoptosis in human leukemia cell lines with a high therapeutic index
... Abstract: Saponin fractions of Quillaja saponaria Molina (QS) have cytotoxic activity against cancer cells in vitro, but are too toxic to be useful in the clinic. The toxic effect was abolished by converting QS fractions ...
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Neuroinflammation in Alzheimer’s disease
... of intracellular signaling pathways involved in inflammatory responses in astrocytes and microglia, including the mitogen-activated pro- tein kinase pathways, nuclear factor-kappa B cascade, and peroxisome ...
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Healthy ageing and depletion of intracellular glutathione influences T cell membrane thioredoxin-1 levels and cytokine secretion
... major intracellular redox buffer, GSH, influences the expression of a specific redox active protein on the T cell surface, Trx-1, its secretion into the extracellular media and the response to mitogen as mea- ...
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Regulation of macrophage subpopulations and their relationship to T cell function in the pathogenesis of asthma.
... It has been suggested that macrophage heterogeneity is generated through a developmental mechanism and that generated macrophage phenotypes are then maintained by systemic environmental constraints (Witsell & Schook ...
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The Predominant CD4+ Th1 Cytokine Elicited to Chlamydia trachomatis Infection in Women Is Tumor Necrosis Factor Alpha and Not Interferon Gamma
... cytokine, tumor necrosis factor alpha (TNF- ␣ ), has also been implicated in protective immunity to chlamydia in murine studies (8–10). TNF- ␣ is a pleiotropic proinflammatory cytokine released from ...
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Increase in IL 21 producing T cells in patients with systemic lupus erythematosus
... IL-21. This might be different in humans. In the present study, we showed that the expression level of BCL6 in sorted T-cells from peripheral blood of SLE patients and HCs was relatively low. This is in line with the ...
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The Level of Viral Antigen Presented by Hepatocytes Influences CD8 T-Cell Function
... Inflammation does not alter the balance between degranu- lation and cytokine production. During natural infection, inflammation will likely affect T-cell responses to infected hepatocytes. Because IFN- ␥ appears ...
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The neurotrophic hepatocyte growth factor attenuates CD8+ cytotoxic T-lymphocyte activity
... multifunctional cytokine that blunts inflamma- tion in a variety of inflammatory T-cell-mediated disease models, suggesting that HGF suppresses a common in- flammatory ...
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