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Intracellular pH (pHi)

The Effect of Curcumin on Intracellular pH (pHi), Membrane Hyperpolarization and Sperm Motility

The Effect of Curcumin on Intracellular pH (pHi), Membrane Hyperpolarization and Sperm Motility

... The pHi plays an important role in modulating mammalian sperm motility (12, 17, ...acidic pHi inhibits sperm motility, which is in agreement with our ...The pHi of ...the pHi of control mu- ...

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Intracellular pH modulates the generation of superoxide radicals by human neutrophils

Intracellular pH modulates the generation of superoxide radicals by human neutrophils

... of intracellular pH (pHi) to superoxide radical (O2-) generation was investigated in chemotactic factor-stimulated human ...in pHi from 7.22 to 7.80. This pHi change was sensitive to ...

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Axial heterogeneity of intracellular pH in rat proximal convoluted tubule

Axial heterogeneity of intracellular pH in rat proximal convoluted tubule

... profile, intracellular pH (pHi) was measured along the superficial PT of the rat under free-flow and stationary microperfusion using the pH-sensitive fluorescence of 4- methylumbelliferone ...

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Regulation of intracellular pH in the rabbit cortical collecting tubule

Regulation of intracellular pH in the rabbit cortical collecting tubule

... the pH sensitive dye 2',7'-bis- (2-carboxyethyl)-5(and-6)carboxyfluorescein (BCECF) and basolateral Cl- removal were used to identify and study intracellular pH (pHi) regulation in each of ...

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The influence of photosynthesis on host intracellular pH in scleractinian corals

The influence of photosynthesis on host intracellular pH in scleractinian corals

... seawater pH was deliberately controlled in order to identify intracellularly driven pHi ...coral pHi with changes in extracellular pH in both the coelenteron lumen and the external seawater ...

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Intracellular pH regulation and proton transport by rabbit renal medullary collecting duct cells  Role of plasma membrane proton adenosine triphosphatase

Intracellular pH regulation and proton transport by rabbit renal medullary collecting duct cells Role of plasma membrane proton adenosine triphosphatase

... membranes, intracellular pH (pHi) regulation and proton extrusion rates were measured in freshly prepared suspensions of rabbit outer MCD ...centrifugation. pHi was estimated fluorometrically ...

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Regulation of Inwardly Rectifying K Channels in Retinal Pigment Epithelial Cells by Intracellular pH

Regulation of Inwardly Rectifying K Channels in Retinal Pigment Epithelial Cells by Intracellular pH

... and intracellular pH modulate the activity of native Kir channels in other cells (Takahashi & Copenhagen, 1995; Rich et ...the pH sensitivity of Kir channels in the ...

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Measurement of Intracellular pH of Skeletal Muscle with pH sensitive Glass Microelectrodes

Measurement of Intracellular pH of Skeletal Muscle with pH sensitive Glass Microelectrodes

... among intracellular pH, transmembrane potential, and extracellular ...and intracellular pH with a minimum of cellular ...a pH-sensitive electrode in a single tip, facilitated the direct ...

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Is intracellular pH a clock for mitosis?

Is intracellular pH a clock for mitosis?

... through prometaphase and continuing to decline through metaphase when parity between microtubule assembly and disassembly leads to mid-cell chromatid pair oscillation, cul- minating in increased microtubule ...

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Control and modulation of intracellular pH in mammalian central neurones

Control and modulation of intracellular pH in mammalian central neurones

... for pH; found with both microelectrode and with pH-sensitive dyes, it would be helpful to use an ...in pH; . The absolute value for pH; can then be calculated from the composition of this ...

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CALCULATION OF INTRACELLULAR pH FROM THE DISTRIBUTION OF 5,5 DIMETHYL 2,4 OXAZOLIDINEDIONE (DMO)  APPLICATION TO SKELETAL MUSCLE OF THE DOG

CALCULATION OF INTRACELLULAR pH FROM THE DISTRIBUTION OF 5,5 DIMETHYL 2,4 OXAZOLIDINEDIONE (DMO) APPLICATION TO SKELETAL MUSCLE OF THE DOG

... Of even greater interest than the calculated CENTRATION IN EXTRACELLULAR TISSUE WATER CE pH for the normal cell are the changes that this value WITH RESPECT TO INTRACELLULAR PH pH, AS A [r] ...

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Regulation of Intracellular pH by Toadfish (Opsanus Beta) Hepatocytes

Regulation of Intracellular pH by Toadfish (Opsanus Beta) Hepatocytes

... Buffering capacity, membrane potential and regulation of pHi under simulated physiological conditions Non-bicarbonate buffering capacity in toadfish hepatocytes was calculated by using t[r] ...

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THE RELATIONSHIP BETWEEN INTRACELLULAR pH AND SWIMMING PERFORMANCE OF BROWN TROUT EXPOSED TO NEUTRAL AND SUBLETHAL pH

THE RELATIONSHIP BETWEEN INTRACELLULAR pH AND SWIMMING PERFORMANCE OF BROWN TROUT EXPOSED TO NEUTRAL AND SUBLETHAL pH

... sublethal pH in the present experiments, then when sufficient power could not be provided by aerobic metabolism, at a relatively low swimming speed, ATP consumption would exceed its production, thus producing ...

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Effect of isoproterenol on intracellular pH of the intercalated cells in the rabbit cortical collecting ducts

Effect of isoproterenol on intracellular pH of the intercalated cells in the rabbit cortical collecting ducts

... 6)carboxyfluorescein. The pHi of beta-IC was significantly decreased with the addition of basolateral 10(-6) M isoproterenol (7.21 +/- 0.04 to 7.05 +/- 0.04), whereas alpha-IC did not show any change. This ...

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Treatment of Lactic Acidosis with Dichloroacetate in Dogs

Treatment of Lactic Acidosis with Dichloroacetate in Dogs

... blood pH and levels of bicarbonate and lactate; the intracellular pH (pHi) in liver and skeletal muscle; cardiac index, arterial blood pressure and liver blood flow; liver lactate uptake and ...

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Intracellular pH regulation in unstimulated Calliphora salivary glands is Na+ dependent and requires V ATPase activity

Intracellular pH regulation in unstimulated Calliphora salivary glands is Na+ dependent and requires V ATPase activity

... A molecular approach was chosen to confirm the existence of an EAAT more strongly than by the pH measurements described above. We amplified a 194bp fragment of EAAT (Cv EAAT) from blowfly salivary gland cDNA. For ...

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Intracellular pH in the toad Bufo marinus following hypercapnia

Intracellular pH in the toad Bufo marinus following hypercapnia

... Non-bicarbonate buffer values determined by titrating tissue homogenates in vitro ft and apparent in vivo non-bicarbonate buffer values f3' calculated from normocapnic and hypercapnic 5 [r] ...

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Effects of bafilomycin A1 on cytosolic pH of sheep alveolar and peritoneal macrophages: evaluation of the pH regulatory role of plasma membrane V ATPases

Effects of bafilomycin A1 on cytosolic pH of sheep alveolar and peritoneal macrophages: evaluation of the pH regulatory role of plasma membrane V ATPases

... and, in cells such as osteoclasts and renal tubular cells, serve to acidify specialized extracellular compartments (Chatterjee et al. 1992; Gluck and Nelson, 1992). In macrophages (m f ), plasma membrane V-ATPases are ...

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Beta adrenergic upregulation of the Na(+) K(+) 2Cl  cotransporter in rat parotid acinar cells

Beta adrenergic upregulation of the Na(+) K(+) 2Cl cotransporter in rat parotid acinar cells

... the pH-sensitive fluorescent dye 2',7'-bis(2-carboxyethyl)-5(6')-carboxyfluorescein to monitor the recovery of the intracellular pH (pHi) of rat parotid acini from an NH4(+)- induced alkaline ...

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Preferential intracellular pH regulation: hypotheses and perspectives

Preferential intracellular pH regulation: hypotheses and perspectives

... coupled pH regulation during compensation for respiratory acid – base disturbances (Brauner and Baker, 2009; Cameron, 1989; Occhipinti and Boron, 2015; Shartau and Brauner, ...

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