Ion Torrent
Development and validation of a variant detection workflow for BRCA1 and BRCA2 genes and its clinical application based on the Ion Torrent technology
... the Ion AmpliSeq BRCA1 and BRCA2 panel (Ion Torrent) and was used to generate target amplicon ...the Ion AmpliSeq primer pools and Ion AmpliSeq HiFi master mix (Ion AmpliSeq kit ...
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Ion Torrent sequencing as a tool for mutation discovery in the flax (Linum usitatissimum L.) genome
... Reads obtained from Ion Torrent PGM sequencing were uploaded to the CLC Genomics workbench platform (CLCbio, Aarhus N, Denmark). Reads were mapped to reference sequences previously confirmed by Sanger ca- ...
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Comparison of somatic variant detection algorithms using Ion Torrent targeted deep sequencing data
... However, identifying somatic variants still remains a challenge due to their low allelic fraction as well as bio- logical and technical issues such as contamination of the sample by non-malignant cells, tumour genetic ...
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Next Generation Ion Torrent Sequencing of Drug Resistance Mutations in Mycobacterium tuberculosis Strains
... Interestingly, Ion Torrent sequencing revealed that 6 of 7 strains contained a rarer glycine residue (D516G) at this position (Table ...ever, Ion Torrent sequencing revealed that 1 of 3 ...
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Parallel tagged amplicon sequencing of transcriptome-based genetic markers for Triturus newts with the Ion Torrent next-generation sequencing platform
... Next-generation sequencing is a fast and cost-effective way to obtain sequence data for nonmodel organisms for many markers and for many individuals. We describe a protocol through which we obtain orthologous markers for ...
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Genotyping of high risk anal human papillomavirus (HPV): ion torrent next generation sequencing vs linear array
... The Ion Torrent NGS assay may not be as sensitive for detecting all HPV type-specific infections as the new multiplex type-specific HPV E7 PCR system [11, 12], but its purpose is to detect the known ...
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Ion Torrent Personal Genome Machine Sequencing for Genomic Typing of Neisseria meningitidis for Rapid Determination of Multiple Layers of Typing Information
... Neisseria meningitidis causes invasive meningococcal disease in infants, toddlers, and adolescents worldwide. DNA sequence- based typing, including multilocus sequence typing, analysis of genetic determinants of ...
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The complete mitogenome of Orcula dolium (Draparnaud, 1801); ultra-deep sequencing from a single long-range PCR using the Ion-Torrent PGM
... unique Ion Express Barcode Adapter (Life Technologies) using the NEBNext Fast DNA & Library Prep Set for Ion Torrent (New England Biolabs), fol- lowing the manufacturers ...
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Ion torrent based transcriptional assessment of a Corynebacterium pseudotuberculosis equi strain reveals denaturing high performance liquid chromatography a promising rRNA depletion method
... the Ion Torrent Personal Genome Machine (PGM™) for cDNA library sequencing, once it is recognized as providing satisfac- tory read lengths in a time- and cost-effective manner (Meldrum et ...
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Dual RNA seq reveals viral infections in asthmatic children without respiratory illness which are associated with changes in the airway transcriptome
... in Ion Proton RNA-seq data Kapa Biosystems mRNA-seq library kits (catalog number KK8421) were used to generate sequencing libraries for Ion Torrent whole transcriptome sequencing for the 21 samples ...
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High throughput profiling identifies clinically actionable mutations in salivary duct carcinoma
... Ion Torrent Ion AmpliSeq Cancer Hotspot Panel v2 (Life Technologies) was used to sequence hotspot re- gions in 50 frequently mutated tumor suppressor genes and ...custom Ion AmpliSeq panel was ...
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A Study of the Microbial Community at the Interface between Granite Bedrock and Soil Using a Culture Independent and Culture Dependent Approach
... A percentage of these sequences were also identified as unclassified (19.10% ± 1.04%). This was potentially due to the short-read lengths, which has made detailed phylogenetic characterisation of the data difficult. Sub- ...
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New recurrent BRCA1/2 mutations in Polish patients with familial breast/ovarian cancer detected by next generation sequencing
... the Ion Torrent PGM sequencer to sequence the coding exons of BRCA1 and BRCA2 genes substan- tially improves the detection rates of a wide spectrum of mutations in Polish patients with familial and/or only ...
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Rapid detection of genetic mutations in individual breast cancer patients by next-generation DNA sequencing
... used Ion Torrent sequencing technology to identify mutations in 80 clinical breast cancer tumors of various ...the Ion Tor- rent sequencing method to genotype cancer samples in a clinical ...setting. ...
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Genomic analysis of four strains of Corynebacterium pseudotuberculosis bv. Equi isolated from horses showing distinct signs of infection
... The pathogenesis of C. pseudotuberculosis is intrinsic- ally linked to its genetic content. Several virulence factors have previously been described in the literature that strongly influence the ability of the bacteria ...
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Comparison of two high-throughput semiconductor chip sequencing platforms in noninvasive prenatal testing for Down syndrome in early pregnancy
... of Ion Torrent PGM was generally better than that of the Proton system ...the Ion Torrent Proton system ...an Ion Proton sequencer could be successful [23]. In addition, the Ion ...
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Connecting the dots between different networks: miRNAs associated with bladder cancer risk and progression
... and Ion Torrent PGM Next-Generation Sequencing an increased mutation rate for TP53, FGFR3, KDR, PIK3CA and ATM were observed, but the mutational status for only TP53 was correlated to the survival ...
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Next generation sequencing: recent applications to the analysis of colorectal cancer
... by Ion Torrent targeted sequencing and found frequent mutations in KRAS, TP53, APC, FBXW7, PI3KCA and, to a lesser extent, in BRAF, CTNNB1, ERBB2 and ...(Ion Torrent, Ampliseq colon and lung) ...
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A Multiplex PCR Based Next Generation Sequencing Approach Has Detected a Common Large Deletion in STS Gene in a Patient with X Linked Ichthyosis
... using ION torrent (Thermo Fisher Scientific) technology, it is strongly recommended to supervise the analysis of mutations in X-linked genes through the Coverage Analysis tool (Thermo Fisher Scientific), ...
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Original Article Targeted next-generation sequencing in cytology specimens for molecular profiling of lung adenocarcinoma
... In this study, we aimed to evaluate NGS using the Ion Torrent technology for detecting mutations in multiple genes related to lung adenocarcinomas EGFR, KRAS, PIK3CA, BRAF, ALK, PDGFRA, [r] ...
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