isocitrate dehydrogenase 1/2
D-2-Hydroxyglutarate producing neo-enzymatic activity inversely correlates with frequency of the type of isocitrate dehydrogenase 1 mutations found in glioma
... All migration assays were performed with Ibidis Culture-Inserts. In each well of the insert, 50,000 cells were seeded. After 24 h, the cells were treated with 10 μg/ml Mitomycin C (Sigma-Aldrich, St. Luis, USA) for ...
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Molecular alterations of isocitrate dehydrogenase 1 and 2 (IDH1 and IDH2) metabolic genes and additional genetic mutations in newly diagnosed acute myeloid leukemia patients
... In a total cohort of 230 consecutive AML patients (36 acute promyelocytic leukemia (APL) and 194 non-APL), 44 patients with IDH mutation were identified (19.13%) by DHPLC showing abnormal chromatogram patterns that were ...
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Mechanism for enhanced 5-aminolevulinic acid fluorescence in isocitrate dehydrogenase 1 mutant malignant gliomas
... oncometabolite, 2-HG, is responsible for multiple activities involved in tumor development [34, ...enzyme 1 (ME1), of which the levels are altered in many type of cancer cells, contribute to NADPH ...
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Isocitrate dehydrogenase 1–snail axis dysfunction significantly correlates with breast cancer prognosis and regulates cell invasion ability
... The isocitrate dehydrogenase (IDH) gene family expresses key functional metabolic enzymes in the Krebs cycle, which can catalyze the conversion of isocitrate to α-ketoglutarate (KG), thus generating ...
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<p>Isocitrate dehydrogenase 1 mutation is associated with reduced levels of inflammation in glioma patients</p>
... The mean preoperative NLR was 3.64±0.22 (median, 2.7; 0.60 ‒ 14.70), the mean PLR was 147.18 ±5.85 (median, 131.9; 47 ‒ 71.9),the mean RDW was 13.21±0.10 (median, 12.90; 11.10 ‒ 18.70), and the mean CRP was 3.59±0.43 ...
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Isocitrate dehydrogenase 1-mutated cancers are sensitive to the green tea polyphenol epigallocatechin-3-gallate
... product of the mutant enzyme [39]. Recognizing the oncogenic role of D-2-HG, IDH1 mut - and IDH2 mut -spe- cific drugs have been developed that inhibit the activity of the mutant enzymes [19]. We recently showed ...
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Original Article A population-based study of high-grade gliomas and mutated isocitrate dehydrogenase 1
... The majority of previously published prognostic studies include only patients with a histological verified glioma, and inclusion is limited to younger patients in good performance status and without comorbidity. In daily ...
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Study of Isocitrate Dehydrogenase 1and 2 Mutations in Adult Egyptian Patients with Denovo Acute Myeloblastic Leukemia, Their Relation to Clinical Characteristics, FLT3/ITD and Nucleophosmin 1 Mutations and Impact on Treatment Outcome
... Background: Acute myeloid leukaemia (AML) is a malignancy that is heterogeneous in nature characterized by genetic abnormalities some of which are established in the diagnosis and prognosis of the disease. An additional ...
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Isocitrate dehydrogenase 1 (IDH1) mutation-specific microRNA signature predicts favorable prognosis in glioblastoma patients with IDH1 wild type
... Here, we used a scoring method to measure the relative expression levels of the 23 miRNAs. Then we divided all of the samples into high-score and low-score groups as shown in Figure 2. We found that the high-score ...
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Prognostic and diagnostic potential of isocitrate dehydrogenase 1 in esophageal squamous cell carcinoma
... Total RNA was extracted from fresh tissues or cells by using TRIzol Reagent (Invitrogen, USA) in accordance with the manufacturer’s instructions. The IDH1 expression of each sample was assessed using the Bio-Rad Single ...
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High expression of 5-hydroxymethylcytosine and isocitrate dehydrogenase 2 is associated with favorable prognosis after curative resection of hepatocellular carcinoma
... The 1-, 3-, and 5-year OS rates in the cohort were ...the 1-, 3-, and 5-year survival rates of the 5-hmC High patients were significantly higher than those of the 5-hmC Low group ...at 1, 3, and 5 ...
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Autocrine IFN I inhibits isocitrate dehydrogenase in the TCA cycle of LPS stimulated macrophages
... at 2 distinct steps, leading to the accumu- lation of citrate, succinate, and itaconate ...of isocitrate dehydrogenase (IDH) (2, 3), the enzyme that converts isocitrate to ...
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Mutations in the isocitrate dehydrogenase 2 gene and IDH1 SNP 105C > T have a prognostic value in acute myeloid leukemia
... All patients were successfully genotyped for IDH1 codon 105 SNP ( rs11554137 ) (Table 1) that was not associated with the IDH mutations (only 7 overlapping cases; 3 in IDH1 and 4 in IDH2 ). The synonymous SNP (GGC ...
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<p>Functional analysis and clinical significance of the isocitrate dehydrogenase 2 gene in papillary thyroid carcinoma</p>
... Cell Counting Kit-8 (CCK-8) assay (Dojindo, Kumamoto, Japan) was employed according to the manufacturer ’ s instructions to measure the effects of drugs on the prolifera- tion of papillary thyroid carcinoma cells. Brie fl ...
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Identification of proteins differentially expressed by glutamate treatment in cerebral cortex of neonatal rats
... type 2, 3, 4, isoci- trate dehydrogenase, and heat shock protein ...type 2 levels were ...animals. Isocitrate dehydro- genase and heat shock protein 60 levels were ...
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Isocitrate dehydrogenase inhibitors in acute myeloid leukemia
... Approximately 40–45% of young and 10–20% of elderly AML patients treated with standard therapy will be cured. There are no more than 10% of relapsed or refractory (R/ R) AML [1–4]. Advancements in next-generation ...
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100 days of marine Synechococcus – Ruegeria pomeroyi interaction, a detailed analysis of the exoproteome
... breakdown of phototrophic DOM over time. Marine Alphaproteobacteria are considered to have a very low extracellular hydrolytic potential due to the low number of enzymes that are encoded in their genomes (Barbeyron et ...
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THE INHERITANCE OF ENZYME VARIANTS FOR TYROSINE AMINOTRANSFERASE, NADP-DEPENDENT MALATE DEHYDROGENASE, NADP-DEPENDENT ISOCITRATE DEHYDROGENASE, AND TETRAZOLIUM OXIDASE IN TETRAHYMENA PYRIFORMIS, SYNGEN 1
... The F, hybrid clones showed both parental isozymes and an isozyme of intermediate mobility, which is presumed to indicate this enzyme is a dimer.. TAIT (1970) has examined t[r] ...
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Multilocus enzyme analysis of Legionella dumoffii
... The following 20 enzymes were measured: esterase 2, esterase 3, esterase 5, esterase 6, isocitrate dehydrogenase, malate dehydrogenase, adenylate kinase, aconitase, phenylalanylleucine p[r] ...
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Biochemical and ultrastructural study of Blastocystis hominis
... Despite the presence of large numbers of mitochondria, activities of the mitochondrial enzymes pyruvate dehydrogenase complex, a-ketoglutarate dehydrogenase complex, isocitrate dehydroge[r] ...
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