Isothermal DNA amplification tests
The detection of Plasmodiophora brassicae using loop-mediated isothermal DNA amplification
... To achieve this aim different molecular tests has been proposed by some researchers. It was already nearly 20 years ago when the single copy DNA se- quence unique to P. brassicae have cloned [13] and then ...
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A Simple Isothermal DNA Amplification Method to Screen Black Flies for Onchocerca volvulus Infection
... LAMP tests for biological substances [72– ...when DNA was extracted using a simple boiling method, rather than using commercially available kits that add a significant cost to the process (as well as time ...
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A novel loop mediated isothermal amplification based test for detecting Neospora caninum DNA
... caninum DNA based on the parasite’s Nc-5 gene; the method’s applicability was also evaluated in naturally in- fected ...caninum DNA, as well as provid- ing a significant contribution; this is the first ...
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Mitochondrial DNA Targets Increase Sensitivity of Malaria Detection Using Loop Mediated Isothermal Amplification
... diagnostic tests (RDTs). Such tests make fast and accurate point-of-care diagnostic testing possible for medical staff with no formal training in microscopy and in areas without well-equipped diagnostic ...
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Development of a Loop Mediated Isothermal Amplification Method for Detection of Histoplasma capsulatum DNA in Clinical Samples
... diagnostic tests have the potential to detect very small amounts of DNA with high spec- ificity, and the LAMP method is both rapid and ...tum DNA in urine from patients with disseminated disease. ...
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The development of loop mediated isothermal amplification targeting alpha tubulin DNA for the rapid detection of Plasmodium vivax
... Discussion The resurgence of vivax malaria in many parts of the world emphasizes the need for a rapid, sensitive, and in- expensive diagnostic method. Furthermore, it needs to be easily performed and to produce readily ...
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Loop-mediated isothermal amplification assay for the detection of Ehrlichia canis DNA in blood samples from dogs #
... loop-mediated isothermal amplification (LAMP) assay was developed for detection of ...canis DNA using LAMP primers targeting the groESL ...Successful amplification was obtained using plasmid ...
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Evaluation of fluorimetry and direct visualization to interpret results of a loop mediated isothermal amplification kit to detect Leishmania DNA
... acid amplification tests (NAATs) have proven to be advantageous in the diagnosis of leishmaniases, allowing sensitive diagnosis of: (i) cutaneous leishmaniasis in long duration lesions and (ii) visceral ...
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Detection of Pathogenic Leptospiral DNA Traces in Canine Sera Serum Samples by Loop Mediated Isothermal Amplification (LAMP)
... spp. DNA traces in ...serovars. DNA extracted from these samples using Chelex-100 resin was used as template for Leptospira ...loop-mediated isothermal amplification (LAMP) ...leptospiral ...
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A loop mediated isothermal amplification assay for the visual detection of duck circovirus
... Bst DNA polymerase, calcein and MnCl 2 , can influence the amplification efficiency of the LAMP assay, which subsequently affects the fluorescence emission of the resulting ...and amplification time. ...
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The Development of a Loop Mediated Isothermal Amplification (LAMP) Procedure for Plague Diagnostic
... This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/ Abstract Plague caused by Yersinia pestis is one of the infectious diseases subject ...
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APPLICATION FOR UNITED STATES LETTERS PATENT ISOTHERMAL AMPLIFICATION OF DNA
... A preferred embodiment for amplification of a plasmid template utilizes a polymerase in the reaction mixture such that the production of amplified product does 10 not require exogen[r] ...
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Loop mediated isothermal amplification (LAMP) shield for Arduino DNA detection
... as DNA or RNA is one of the privileged analytical methods for confirming the presence of parasites, viruses, bacterial infections, or for example for food ...of DNA or RNA is too low to be detected, their ...
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Detection of Human Herpesvirus 7 DNA by Loop Mediated Isothermal Amplification
... loop-mediated isothermal amplification (LAMP), initially developed for the detection of human herpesvirus 7 (HHV-7), was evaluated in this ...HHV-7 DNA, neither HHV-6 nor human cytomegalovirus ...
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An isothermal DNA amplification method for detection of Onchocerca volvulus infection in skin biopsies
... An isothermal, loop-mediated amplification method has been designed using the mitochondrial ...synthetic DNA containing the targeted sequence show a non-linear dose-response curve, as is usually the ...
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Rapid Diagnosis of Human Herpesvirus 6 Infection by a Novel DNA Amplification Method, Loop Mediated Isothermal Amplification
... target DNA, its specificity is extremely ...high amplification effi- ciency, due in part to its isothermal nature: there is no time lost due to changes in temperature, the reaction can be conducted ...
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A paper and plastic device for the combined isothermal amplification and lateral flow detection of Plasmodium DNA
... human DNA and other blood ...extracting DNA in a manner compatible with the RPA nfo assay will need to be ...and isothermal amplification on clinical malaria samples with minimal need for ...
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Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection
... Dra1 DNA region of S. haematobium and amplification success was easily detected by a semi quantitative colouri- metric change on LF ...strips. Amplification was detectable after only 5 min of ...
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Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis
... The LAMP-Au-nanoprobe strategy was capable to specif- ically identify MTBC members and mutations associated to RIF resistance in Mtb within 90 minutes of DNA extraction. Our technique has proven to be a fast and ...
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Loop mediated isothermal amplification for the detection of goose circovirus
... loop-mediated isothermal assay (LAMP) that allows simple, sensitive and specific detection of goose circovirus DNA with naked eye or under UV-light as soon as 30 min using only waterbath or dry block ...
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