Kluyveromyces marxianus
Engineering Kluyveromyces marxianus as a Robust Synthetic Biology Platform Host
... yeast Kluyveromyces marxianus possesses a number of beneficial traits that make it a prom- ising alternative to ...K. marxianus is the fastest-growing eukaryotic organism known (2), is thermotolerant, ...
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Genetic and physiological basis for antibody production by Kluyveromyces marxianus
... Kluyveromyces marxianus is a thermotolerant, crabtree‑negative yeast, which preferentially directs metabolism ...K. marxianus has great potential for engineer‑ ing to produce various materials under ...
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Removal of aromatic inhibitors produced from lignocellulosic hydrolysates by Acinetobacter baylyi ADP1 with formation of ethanol by Kluyveromyces marxianus
... stage, Kluyveromyces marxianus was then used in the same reactor to convert the remaining sugar, glu- cose, into ...K. marxianus was inhibited by ...K. marxianus grows at 42 °C and a pH of ...
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Characterization of the Kluyveromyces marxianus strain DMB1 YGL157w gene product as a broad specificity NADPH-dependent aldehyde reductase
... The open reading frame YGL157w in the genome of the yeast Kluyveromyces marxianus strain DMB1 encodes a putative uncharacterized oxidoreductase. However, this protein shows 46% identity with the ...
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Purification And Evidence For The Involvement Of Tryptophan Residue At The Catalytic Site Of Â-Fructofuranosidase From A Thermotolerant Yeast Kluyveromyces Marxianus Ncyc 2675
... β Fructofuranosidase from Kluyveromyces. marxianus NCYC 2675 was purified using hydrophobic interaction chromatography. The purified enzyme had specific activity of 2116 µmoles/min/ml. Molecular weight was ...
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Efficient growth of Kluyveromyces marxianus biomass used as a biocatalyst in the sustainable production of ethyl acetate
... and Kluyveromyces marxianus produce this ester in larger ...K. marxianus is the most promising candidate for large-scale ester production since this dairy yeast with GRAS status grows quickly, ...
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Enhanced fermentative performance under stresses of multiple lignocellulose-derived inhibitors by overexpression of a typical 2-Cys peroxiredoxin from Kluyveromyces marxianus
... yeast Kluyveromyces marxianus, which possesses the advantages of high-temperature resistance, rapid growth rate, and diversity of substrates, identi- fied lots of differentially expressed genes (DEGs) ...K. ...
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Alleviation of catabolite repression in Kluyveromyces marxianus: the thermotolerant SBK1 mutant simultaneously coferments glucose and xylose
... engineered Kluyveromyces marxianus over expressed the xylose-specific transport- ers (GAL2-N376F) and simultaneously cofermented glucose and xylose, producing ...K. marxianus, is a robust strain used ...
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Prosopis Juliflora Bark - An Alternate Feedstock in the Production of Bioethanol Using Thermo Tolerant Yeast Kluyveromyces Marxianus
... The delignified biomass of Prosopis juliflora was exposed to Kluyveromyces marxianus in the presence of cellulase enzyme by SSF process and inoculum concentrations were varied (1%,3% and 5% (v/v)). During ...
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SELECTION AND IDENTIFICATION OF A NOVEL THERMOTOLERANT Kluyveromyces marxianus STRAIN FOR VALORIZATION OF LACTOSE WASTE INTO ETHANOL
... Sustainable production of ethanol as alternative renewable fuels, there is a need for a yeast strain which can convert the non-food substrate into ethanol economically. Extensive screening program for such yeast for ...
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A novel Kluyveromyces marxianus strain with an inducible flocculation phenotype
... Flocculation is a very useful phenotype for industrial yeast strains, since it facilitates cell harvest and represents an easy way of cell immobilization in continuous fermentation processes. The present work represents ...
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CRISPR–Cas9-enabled genetic disruptions for understanding ethanol and ethyl acetate biosynthesis in Kluyveromyces marxianus
... K. marxianus. K. marxianus is known to have a high capacity for DNA repair by nonhomologous end joining (NHEJ), which can limit the rapid generation of targeted knockout libraries by traditional genome ...
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Transcriptional analysis of Kluyveromyces marxianus for ethanol production from inulin using consolidated bioprocessing technology
... K. marxianus Y179 were analyzed using a phylogenetic tree ...K. marxianus was more sensitive to the reactive oxygen species ...K. marxianus Y179 was dra- matically up-regulated (over 10-fold) under ...
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The transcription factors Hsf1 and Msn2 of thermotolerant Kluyveromyces marxianus promote cell growth and ethanol fermentation of Saccharomyces cerevisiae at high temperatures
... To determine stress-related TFs in K. marxianus, we did protein–protein BLAST with the accession numbers of stress-related TFs in S. cerevisiae as inputs. For each stress-related TF in S. cerevisiae in this study, ...
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Genetic basis of the highly efficient yeast Kluyveromyces marxianus: complete genome sequence and transcriptome analyses
... Genes regulated under a high-temperature condition To clarify the thermotolerant mechanism of K. marxia- nus, it is necessary to consider the expressional alteration of whole genomic genes at high temperature. Under the ...
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Kluyveromyces marxianus developing ethanol tolerance during adaptive evolution with significant improvements of multiple pathways
... K. marxianus is able to utilize a variety of carbon sources, including inulin, xylose, and lactose [12, 13], which cannot be used by ...K. marxianus in a strict anaerobic environment at 37 °C reached ...
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Synergistic effect of thioredoxin and its reductase from Kluyveromyces marxianus on enhanced tolerance to multiple lignocellulose-derived inhibitors
... K. marxianus can enhanced tolerance to mul- tiple lignocellulose-derived inhibitors and achieved the remarkable ethanol fermentative profiles under inhibi- ...
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Inhibition of the lactose to ethanol fermentation of Kluyveromyces marxianus Y113 and attempts at its alleviation through media improvement : a thesis presented to Massey University in fulfillment of the thesis requirement for the degree of Master of Technology
... trace elements as per Difco medium salts as per Difco medium The performance of this medrun kas compared to lactosr: brotlt and whey permeate media in Run B and the results; are presente[r] ...
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Inhibition of the lactose to ethanol fermentation of Kluyveromyces marxianus Y113 and attempts at its alleviation through media improvement : a thesis presented to Massey University in fulfillment of the thesis requirement for the degree of Master of Technology
... Introduction 5-2-2 Results and Discussion Comparison of Whey Permeate and Demineralised Whey 5.3 Permeate for the Production of Ethanol.. 5.3.1 Introduction 5.3.2 Results and Discussion [r] ...
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Proteomic profiling and integrated analysis with transcriptomic data bring new insights in the stress responses of Kluyveromyces marxianus after an arrest during high-temperature ethanol fermentation
... K. marxianus during the late stage of high-temperature ethanol fer- ...K. marxianus: such as decreases in transcription, translation and oxidative phosphoryla- tion, alterations in cellular fatty acid ...
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