Knockin mice
Measles Virus Infection of SLAM (CD150) Knockin Mice Reproduces Tropism and Immunosuppression in Human Infection
... transgenic mice expressing Cre recombinase from the CAG pro- moter (provided by ...heterozygous knockin mice were crossed with C57BL/6 mice to remove the Cre ...
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Mutation-induced loss of APP function causes GABAergic depletion in recessive familial Alzheimer’s disease: analysis of Osaka mutation-knockin mice
... receptor, was dissolved to 10 μg/ml in 0.5% low-viscosity car- boxymethylcellulose (CMC; Sigma). Diazepam is usually pre- scribed to adult humans at 2 to 10 mg orally 2 to 4 times a day for anxiety and seizures ...
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Essential role for CCR6 in certain inflammatory diseases demonstrated using specific antagonist and knockin mice
... of mice treated with Vaseline (first column), isotype + imiquimod (IMQ) (second column), anti–mIL-17 mAb + IMQ (third column), and h6H12 + IMQ (fourth column) and analyzed by flow ...2 mice (6 mice ...
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Impaired synaptic clustering of postsynaptic density proteins and altered signal transmission in hippocampal neurons, and disrupted learning behavior in PDZ1 and PDZ2 ligand binding-deficient PSD-95 knockin mice
... KI mice neurons were acquired without noise filters following fluorescence excitation due to the weak signals, whereas images for WT neurons were acquired with noise ...KI mice during ...KI mice at ...
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Knockin mouse with mutant Gα11 mimics human inherited hypocalcemia and is rescued by pharmacologic inhibitors
... generated knockin mice harboring the point mutation GNA11 ...mutant mice faithfully replicated human ...mutant mice, suggesting that the gain-of-function effect of GNA11 R6OC is partly ...
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Genetic Requirement For The Rna Helicase Mov10l1 In Pirna Biogenesis
... Total RNA was extracted from testes using Trizol reagent (Invitrogen), treated with DNase I (Invitrogen), and reverse transcribed to cDNA using M-MLV reverse transcriptase (Promega) according to the manufacturer’s ...
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CRISPR/Cas9 mediated gene editing ameliorates neurotoxicity in mouse model of Huntington’s disease
... in mice can lead to embryonic ...mHTT-expressing mice (HD140Q-knockin mice) using CRISPR/Cas9-mediated inactivation effectively depleted HTT aggregates and attenuated early ...
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Genetic substitution of Cdk1 by Cdk2 leads to embryonic lethality and loss of meiotic function of Cdk2
... the knockin background did not rescue the phenotypes caused by the substitution of Cdk1 by ...heterozygous knockin mice ( Cdk1 +/Cdk2KI ), in which one copy of Cdk2 is expressed from the Cdk1 locus ...
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STAT3 enhancing germline mutations contribute to tumor extrinsic immune evasion
... from knockin mice, knockout mice, and genetically engi- neered knockin mouse models for lung and breast cancers, our study illustrates a pleiotropic effect of cancer-associated STAT3- ...
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A sodium channel knockin mutant (NaV1 4 R669H) mouse model of hypokalemic periodic paralysis
... generated knockin mice carrying the ortholog of the gene encoding the Na V ...R669H mice). Homozygous R669H mice had a robust HypoPP phenotype, with transient loss of muscle excitability and ...
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Androgen receptor polyglutamine expansion drives age dependent quality control defects and muscle dysfunction
... disorder of the neuromuscular system caused by a CAG/polyglutamine (polyQ) expansion in the androgen receptor (AR) gene. Here, we used RNA-sequencing (RNA-Seq) to identify pathways that are disrupted in diseased muscle ...
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Analysis of hepatic gene transcription in mice expressing insulin insensitive GSK3
... knock-in) mice are viable and display no signs of overt diabetes, despite insulin being unable to stimulate glycogen synthase in skeletal muscle of GSK3β knockin mice ...
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MEK ERK pathway modulation ameliorates disease phenotypes in a mouse model of Noonan syndrome associated with the Raf1L613V mutation
... Hypertrophic cardiomyopathy (HCM) is a leading cause of sudden death in children and young adults. Abnormalities in several signaling pathways are implicated in the pathogenesis of HCM, but the role of the ...
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Inhibition of Coxsackievirus associated dystrophin cleavage prevents cardiomyopathy
... generated knockin mice harboring a mutation at the protease 2A cleavage site of the dystrophin gene, which prevents dystrophin cleavage following CV ...wild-type mice, we found that mice ...
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Loss-of-function phenotype of a PKCθT219A knockin mouse strain
... knockout mice defined a reduced T cell population in the thymus indicating an involvement of PKCθ in the positive selection process during thymocyte development [12, ...T219A knockin mice revealed no ...
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Progerin elicits disease phenotypes of progeria in mice whether or not it is farnesylated
... in mice harboring a knockin HGPS mutation (Lmna HG/+ ...created knockin mice expressing nonfarnesylated progerin (Lmna nHG/+ ...nHG/+ mice developed the same disease phenotypes observed ...
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Targeted gene knockin in zebrafish using the 28S rDNA-specific non-LTR-retrotransposon R2Ol
... R2Ol-injected embryos to adulthood and crossed the adult fish with an uninjected wild type (WT) fish to yield F1 em- bryos (Fig. 1b, (4) R2Ol-r100-v, Fig. 2a, Additional file 1: Figure S3-ii). Genomic DNA was extracted ...
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Hotspot SF3B1 mutations induce metabolic reprogramming and vulnerability to serine deprivation
... Knockin of mutant SF3B1 alters the transcriptome of breast epitheli- al cells. SF3B1 is recurrently mutated in 2% of breast cancers and associates with poor prognosis in subgroups of patients, but the functional ...
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Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off target effects
... To gain insight into the targeting specificity and the extent of the off-target effects of CRISPR/Cas9 system in the bovine genome, we performed ChIP-seq for dCas9 protein binding site d[r] ...
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Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9 mediated double stranded DNA cleavage
... well-known difficulty in editing human iPSCs in com- parison to 293 T cells [26]. We first chose to target CTNNB1 , a pivotal gene in the canonical WNT pathway that is constitutively expressed in iPSCs and other cells. ...
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