Kruppel-like factor 1 (erythroid)
Kruppel like factor 4 is critical for transcriptional control of cardiac mitochondrial homeostasis
... and 1 NRRE site on the 1,766-bp mouse Cycs promoter used in this study, we first performed 5′-end serial truncation to locate the crucial KRE sites and found that the transactivation synergy was significantly ...
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Berberine regulates the protein expression of multiple tumorigenesis-related genes in hepatocellular carcinoma cell lines
... including Kruppel‑like factor 6 (KLF6), activating transcription factor 3 (ATF3) and p21, while down‑regulating the expression of selected oncogenes, including E2F transcription factor ...
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Left ventricular hypertrophy in experimental chronic kidney disease is associated with reduced expression of cardiac Kruppel-like factor 15
... growth factor (CTGF)); and (2) that ACE inhibition would reverse LVH and fibrosis in CKD and restore cardiac KLF15 levels and corresponding changes in transcriptional regulators and hypertrophic and pro-fibrotic ...
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Kruppel-like factor 2 suppresses mammary carcinoma growth by regulating retinoic acid signaling
... 6.5 and 10 mg/ml proteinase K. DNA was extracted using phenol-chlorofrom. PCRs were carried out using Go Taq DNA polymerase (Promega). The regions that contained KLF2 response elements regions (CACCC) were amplified by ...
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Kruppel like factor 5 is required for perinatal lung morphogenesis and function
... β 1 in the respiratory epithelium have morphological similarities, including disrupted alveolar sacculation and enhanced bronchiolar smooth muscle cell differentiation (Zeng et ...
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Progression of diet induced nonalcoholic steatohepatitis is accompanied by increased expression of kruppel like factor 10 in mice
... necrosis factor (TNF)α, ChREBP, sterol regulatory element binding protein (SREBP)-1c, collagen α1(I) (Col1α(I)) and smooth muscle α-actin (αSMA) were used with 18S as internal ...Germany), 1 uL of Gene ...
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Statin induced Kruppel like factor 2 expression in human and mouse T cells reduces inflammatory and pathogenic responses
... Statins increase mouse T cell KLF2 expression by a mevalonate/isoprenoid-dependent pathway. (A and B) Naive OT-1 T cells were treated for 18 hours with vehicle (–), fluvastatin (F) (10 μM), lovastatin (L) (10 μM), ...
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Original Article Association of Kruppel-like factor 4 expression with the prognosis of esophageal squamous cell carcinoma patients
... The expression of KLF4 was examined by immu- nohistochemical (IHC) staining methods. Esophageal carcinoma tissues were fixed with 4% paraformaldehyde and embedded with par- affin using standard methods. IHC was carried ...
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Original Article Increased expression of Zinc finger protein 267 in non-alcoholic fatty liver disease
... The Kruppel-like transcription factor (KLF) family is involved in the regulation of diverse aspects of cellular development, differentiation, growth, and activation ...
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Endothelial Kruppel like factor 4 protects against atherothrombosis in mice
... either factor in endothelial biology and atherothrombo- sis has not been ...this factor as a key determinant of experi- mental vascular inflammation and ...
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Tumor-suppressive role of Kruppel-like factor 4 (KLF-4) in colorectal cancer.
... Figure 4. Transfection with KLF-4-specific siRNA promotes the proliferation of CRC cells in vitro. A. siRNA- KLF-4 transfection for 24 h significantly suppressed the KLF-4 mRNA levels in HT-29 cells when compared to the ...
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Kruppel like factor 15 is critical for vascular inflammation
... the Kruppel-like family of factors as important regulators of inflammation ...vasculitis like experimental atherogenesis remains an important unanswered ...
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Integration of flow dependent endothelial phenotypes by Kruppel like factor 2
... dramatically reduced the IL-1b–mediated increase of cell surface TF, the primary cellular initiator of blood coagula- tion (Figure 3H) (26). The coordinated regulation of these critical anti- and procoagulant surface ...
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Common variants of NFE2L2 gene predisposes to acute respiratory distress syndrome in patients with severe sepsis
... The importance of redox balance in the pathogenesis of ARDS, as well as the implication of NFE2L2 in disease susceptibility or outcome, is supported by the identifica- tion of variants in a few genes involved in the ...
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Krüppel-like factors in cancer progression: three fingers on the steering wheel.
... successful reprogramming to pluripotency [28]. Indeed, in MCF-10A normal mammary epithelium, KLF4 was shown to activate CDH1 transcription through binding of CACCC consensus sequences in the proximal promoter of the CDH1 ...
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Nuclear Klf4 accumulation is associated with cetuximab drug resistance and predicts poor prognosis of nasopharyngeal carcinoma
... After deparaffinization and rehydration, the paraffin- embedded sections were subjected to antigen retrieval for 2 min. Then, the slides were incubated overnight at 4 °C with the following primary antibodies: (dilution ...
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Acute myeloid leukemia and transcription factors: role of erythroid Krüppel-like factor (EKLF)
... AMLs with CEBPA mutations show many characteris- tics also found in the EKLF-positive AML group, such as a high percentage of erythroblasts and favorable out- come. Although the study population was small, we found that ...
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Mitochondrial reactive oxygen species and cancer
... Mitochondrial ROS modify metabolism The interplay between ROS levels and cellular metabolism is tightly regulated. Metabolic processes produce ROS, particu- larly in the mitochondria, thus metabolic fluxes need to be ...
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Network analysis of KLF5 targets showing the potential oncogenic role of SNHG12 in colorectal cancer
... According to the manufacturer’s protocol, TRIzol reagent (Ambion, Carlsbad, CA, USA) was used to extract total RNA from tissues and cultured cells. We reverse-tran- scribed 2 μg total RNA into cDNA using the PromegaGo- ...
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Targeting insulin-like growth factor axis in hepatocellular carcinoma
... The majority of IGF-1 is synthesized in the liver under the influence of growth hormone, which is a major pro- moter of postnatal growth [18]. However, deletion of liver specific IGF-1 gene in mice showed ...
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