L. donovani
Experimental induction of paromomycin resistance in antimony-resistant strains of L. donovani : outcome dependent on in vitro selection protocol
... promastigote growth medium after scraping the macrophages to mechanically release amastigotes surviving the highest drug concentration and allow back-transformation into promastigotes. Once promastigote growth was ...
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In vitro stimulation of metacyclogenesis in Leishmania braziliensis, L donovani, L major and L mexicana
... Infections of L. donovani (MHOM } ET } 67 } HU3 ; LV9) and L. braziliensis (MHOM } BR } 84 } LTB300) were maintained in hamsters. L. mexicana (MNYC } BZ } 62 } M379) and L. major clone ...
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Histopathological characterization of L. donovani infection in RAG2-/-mice
... Leishmania donovani (MHOM/NP/03/D10) was obtained from National BioResource Project at Nagasaki ...of L. donovani D10 were cultured in TC 199 medium at pH ...
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Conducting ethical clinical trials for <em>L. donovani</em> in the Bihar region of India
... Leishmania donovani is a flagellated protozoan parasite of animals and humans respon- sible for the presentation of ...leishmaniasis. L. donovani has a divalent life cycle consisting of a ...
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Leishmania mortality in sand fly blood meal is not species specific and does not result from direct effect of proteinases
... First we tested the killing effect of midgut homogenates from four sand fly species dissected 24 h pbm. All para- site forms tested (amastigotes, transition forms and pro- mastigotes) of both Leishmania species were ...
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Leishmania donovani populations in Eastern Sudan: temporal structuring and a link between human and canine transmission
... Visceral leishmaniasis in Sudan is a major and on-going public health problem [6]. Molecular epidemiological studies like ours can have a significant role in guiding and informing public health professionals. Our first ...
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Molecular Preadaptation to Antimony Resistance in Leishmania donovani on the Indian Subcontinent
... and L. donovani transmission has only been reported recently (16); hence we may hypothesize that antimonial pressure was historically lower in these regions than in the ...
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Metabolic variation during development in culture of Leishmania donovani promastigotes
... clinical L. donovani isolates with different antimonial sensitivity has shown a shift towards higher unsaturation of PC lipids in drug-resistant clones, suggesting an increase in membrane fluidity that may ...
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An outbreak investigation of visceral leishmaniasis among residents of Dharan town, eastern Nepal, evidence for urban transmission of Leishmania donovani
... Dharan can be attributed to transmission within the town. The vector P. argentipes and the parasite L. donovani have been identified inside the town. Vector control activities (IRS) appear to have been ...
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Kinesin Motor Domain of Leishmania donovani as a Future Vaccine Candidate
... to L. donovani infection by producing different cytokines of Th1 and Th2 ...by L. donovani in humans induces T-cell anergy as assessed by the depression of delayed-type hypersensitivity re- ...
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Investacte the Role of Weissella Confuse on TNF-α in Serum and Liver Tissue of Mice Infected with Leishmania Donovani
... parasite/ml L. donovani promastigotes by injection intraperitonial [10]. After fourteen days the parasite infection six mice were sacrificed, and prepared impression smears on a slide from the liver,then ...
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Evaluation of antileishmanial potential of Gentiana kurroo Royle by in vitro and in silico methods
... five L. donovani drug targets for the preliminary screening of thirteen reported phyto- constituents by in silico protein-ligand docking to find out the compound responsible for in vitro antileishmanial ...
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Xenodiagnosis of Leishmania donovani in BALB/c mice using Phlebotomus orientalis: a new laboratory model
... of L. donovani is predominantly driven by asymptomatic persons, whose incidence is much higher than symptomatic VL cases ...of L. donovani (k-DNA qPCR positive) had sufficiently high ...
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COMPARATIVE METABOLOMIC INVESTIGATION OF LEISHMANIA MAJOR AND HOMOSAPIENS: AN IN-SILICO TECHNIQUE TO DISCOVER AND DEVELOP NOVEL DRUG TARGETS
... of L donovani and L major, FAD binding domain of TR in L major is almost conserved, except a mutant motif FFA is present instead of ALV in L donovani and LFA in ...
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Rapid identification of causative species in patients with Old World leishmaniasis
... Conventional methods for the identification of species of Leishmania parasite causing infections have limitations. By using a DNA-based alternative, the present study tries to develop a new tool for this purpose. ...
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Enzyme Linked Immunosorbent Assay Based on Soluble Promastigote Antigen Detects Immunoglobulin M (IgM) and IgG Antibodies in Sera from Cases of Visceral and Cutaneous Leishmaniasis
... In this study 284 human and 52 canine sera were tested by an antibody capture micro-ELISA technique using a soluble an- tigen fraction derived from culturing either L. donovani or L. mexicana ...
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... The rLdhyb and rLdhyc encoding sequences (NCBI Accession no. XP_003860226.1 and NCBI Accession no. XP_003861271.1 respectively) from L. donovani genomic DNA was amplified by PCR. A set of forward (5’TTT GGA ...
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The isolation of antiprotozoal compounds from Libyan propolis
... The MPLC fractions obtained for were also tested against L. donovani. The results are shown in table 2 and it can be seen that the IC50 values are somewhat variable which can be attributed to the difficulty ...
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Diagnosis of Visceral Leishmaniasis by Enzyme-Linked Immunosorbent Assay Using Urine Samples
... anti-L. donovani IgG was set as the mean plus 3 standard deviations of log (unit ⫹ 1) values of the healthy controls from areas of nonendemicity and of the Japanese controls for urine and serum, ...
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Epidemiology of visceral leishmaniasis
... Leishmania donovani infections are restricted to the (sub-)tropics of Asia and Africa, where transmission is mostly anthroponotic, while Leishmania infantum occurs in the drier parts of Latin America as well as in ...
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