Label-Free Techniques for Protein Detection
Label-free quantitative proteomics trends for protein–protein interactions
... identify protein–protein ...affinity-tagging protein purification (AP) is the most common, as well as approaches based on the use of reporter expression or ...the protein reconstitution ...
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Label-Free Electrochemical Sensing Platform for the Detection of Protease
... a label-free strategy for the design of electrochemical biosensors for protease detection based on the change of interfacial features of the ...
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Label-free Voltammetric Immunosensor for Prostate Specific Antigen Detection
... PSA detection have been reported including enzyme-linked immunosorbent-assays (ELISA) [26], electrochemical im- munoassays [24, 27–29], chemiluminescent immunoassays [30] and bioluminescent immunoassay ...priate ...
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Directly immobilized DNA sensor for label free detection of herpes virus
... for label-free detection of herpes virus Phuong Dinh Tam 1,2 , Mai Anh Tuan 2 , Nguyen Duc Chien 3 1 Hanoi Advanced School of Science and Technology, Hanoi University of Technology, Hanoi, Vietnam; 2 ...
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Automated Selected Reaction Monitoring Software for Accurate Label-Free Protein Quantification
... virulence. 27 Growing S. pyogenes with plasma changes the proteome homeostasis of the bacterium but also represents a vastly di fferent sample as there are a substantial amount of plasma proteins present. Nevertheless, ...
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Label-Free Electrochemical Sensor for MicroRNAs Detection with Ferroceneboronic Acids as Redox Probes
... specific detection of oligonucleotide ...a label-free electrochemical sensor for miRNAs detection based on the difference in the structure of RNA and ...for label-free ...
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A simple peak detection and label-free quantitation algorithm for chromatography-mass spectrometry
... 1) label-free quantitation using MS1; 2) widely used and freely available; 3) run on Windows OS, therefore, MZmine 2 [14], MSight [15], SuperHirn [16] and OpenMS [17] were chosen finally for comparison in ...
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Fundamental research on the label free detection of protein adsorption using near infrared light responsive plasmonic metal nanoshell arrays with controlled nanogap
... the detection of biological ...simple detection systems allow them to be applied ...sensing techniques are based on various sensing transduction mechanisms, fluorescence, light absorption and ...
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Reproducible and label-free biosensor for the selective extraction and rapid detection of proteins in biological fluids
... (rHuEPO) detection tools that improve on the current laborious EPO detection techniques are in high demand for both clinical and sports ...Raman detection, a low RSD of ...
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Probe-free label system for rapid detection of Cronobacter genus in powdered infant formula
... rapid detection method was developed which combined loop-mediated isothermal amplification with lateral flow assay for detection of Cronobacter species in powdered infant ...final detection time of ...
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Label-free and Aptamer-based Surface Enhanced Raman Spectroscopy for Detection of Food Contaminants
... a label-free aptamer-based SERS technique was developed to detect two foodborne pathogens, salmonella enteritidis and listeria monocytogenes in ...the detection was solely based on the Raman peaks ...
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Label-Free Time-Gated Luminescent Detection Method for the Nucleotides with Varying Phosphate Content
... triphosphate, label-free, K-Ras, apyrase, terbium Abstract. A new label-free molecular probe for luminescent nucleotide detection in neutral aqueous solution is ...direct ...
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Label-Free Impedimetric Immunosensors for Detection of Snake Venoms Using Polyaniline as a Transducer Substrate
... MS.ID.004190. ARTICLE INFO Abstract Two label-free impedimetric immunosensors were developed to be employed in the specific diagnosis of snakebite. To produce the devices, a conductive substrate was ...
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A graphene-based glycan biosensor for electrochemical label-free detection of a tumor-associated antibody
... control protein (Con ...of detection achieved ...the label-free assay system in an array format of analysis and to compare it with other commercially available label-free systems ...
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Label Free C-reactive Protein Detection Based on An Electrochemical Sensor for Clinical Application
... The step-by-step construction of a receptive surface can be well characterized via an EIS measurement. The electrostatically or sterically imposed charge transportation restrictions with the fabrication of the films are ...
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Development of Electrochemical Aptasensor for Label-Free Glioma Cell Detection
... This work explored the fabrication of a functionalized graphene-and-aptamer AS1411-based electrochemical sensor to achieve tag-free cancer cell determination. The proposed electrochemical aptasensor could ...
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Identification of potential protein biomarkers for early detection of pregnancy in cow urine using 2D DIGE and label free quantitation
... create protein–protein interaction network for iden- tified urinary proteins, offline software tool Cytoscape was used along with plug-in ...and protein kinase ...
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Nanomechanical Label Free Micro RNA Detection For Cancer And Liver Injury Diagnosis
... miRNA detection in blood based samples The absolute deflection values for the DILI study was plotted in figures ...4.5. Detection of miRNA in blood-based samples produces a specific differential ...serum ...
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A label-free Electrochemical Immunosensor for Anti-Mullerian Hormone Detection on Graphene-Au Nanocomposite
... This study proposed a novel electrochemical immunosensor modified by AuNPs/PEDOT/GR to detect AMH. The fabrication of this nanocomposite included two steps. A facile liquid–liquid interfacial polymerization was used to ...
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Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration
... in test solution. The deposition of Con A on the surface was carried out using the layer-by-layer method with polyethyleneimine polycation as an initial layer. The sensor surface characterization by means of ...
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