latex beads
Monoclonal antibodies specific for Clostridium difficile toxin B and their use in immunoassays
... difficile culture filtrate and Mono Q-purified fractions were incubated with these latex beads to bind the cytotoxin and then centrifuged to remove the beads, a drastic decrease of the t[r] ...
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Isolation of anti-extra-cellular vesicle single-domain antibodies by direct panning on vesicle-enriched fractions
... of latex beads were coated over- night at 4 °C with 100 µg of ...H1/H6-GFP. Beads were washed three times with PBS and blocked first for 30 min at room temperature with 200 mM glycine and then 30 min ...
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Characterization of immortalized MARCO and SR-AI/II-deficient murine alveolar macrophage cell lines
... trose (10 mM) and HEPES (20 mM), adding CaCl 2 (0.3 mM) and MgCl 2 (1.0 mM). In a 96-well ultra low adher- ent plate (Corning Inc., Corning, NY): fifty microliters of macrophages (100K cells) were mixed with 50 μl of TiO ...
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Candida albicans Als3, a Multifunctional Adhesin and Invasin
... Moreover, latex beads coated with the recombinant N terminus of Als3 (rAls3-N) are effi- ciently endocytosed by these host cells, whereas control beads coated with bovine serum albumin (BSA) are not ...
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Multiplex lateral flow immunoassay: an overview of strategies towards high-throughput point-of-need testing
... Strategies to multiplexing that have developed primary for other immuno-analytical techniques can be transferred to setting xLFIA. For instance, the development of antibodies or other recognition elements that are ...
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Particle agglutination assays for rapid detection of fibronectin, fibrinogen, and collagen receptors on Staphylococcus aureus
... Latex beads 0.8-,um diameter; Difco Laboratories were coated with fibronectin, fibrinogen, collagen type denatured collagen gelatin and evaluated in a particle agglutination assay PAA fo[r] ...
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Manipulation and trapping of sub micron bioparticles using dielectrophoresis
... Electrodes were manufactured on glass microscope slides (25 mm 3 75 mm) using standard photolithographic and electron-beam lithographic techniques [13]. Electrodes consisting of layers of 10 nm titanium, 10 nm palladium ...
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Phagocytosis of hemozoin by RAW 264.7 cells, but not THP-1 cells, promotes infection by Leishmania donovani with a nitric oxide-independent mechanism
... During its intra-erythrocytic development, the malarial parasite Plasmodium falciparum synthesizes insoluble hemozoin (HZ) crystals that are released into the circulation upon rupture of parasitized red blood cells, and ...
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CELL SURFACE PROTEINS OF CRYPTOCOCCUS NEOFORMANS MEDIATE ADHERENCE AND INTERNALISATION INTO MAMMALIAN CELLS
... Cryptococcus neoformans is an encapsulated fungal pathogen that causes severe disease primarily in immunocompromised patients. Adherence and internalisation of microbial pathogens into host cells often begin with ...
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EZH2 suppression in glioblastoma shifts microglia toward M1 phenotype in tumor microenvironment
... fluorescent latex beads were added to microglia for 90 ...fluorescent beads contained within PM (< 2 beads/cell, 2 – 10 beads/cell, and > 10 beads/cell) in 20 randomly ...
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Amyloid beta Induced Astrocytic Phagocytosis is Mediated by CD36, CD47 and RAGE
... fluorescently-labelled latex beads and fluorescently- labelled (FITC-tagged) ...of latex beads by astrocytes, in addition to decreasing uptake of beads even in the absence of ...
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Rapid and Quantitative Detection of Zoonotic Influenza A Virus Infection Utilizing Coumarin-derived dendrimer-based Fluorescent Immunochromatographic Strip Test (FICT)
... with latex beads, fluorescent emission covering broad output spectral ranges was obtained which provided a distinct advantage of easy discrimination of the fluorescent emission of the latex ...
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Microbial partitioning in urban stormwaters
... Calibration of 500 rpm (73g) partitioning technique for latex beads ... Comparison of Colilert and qPCR measures of E. coli concentration ... Average settleable fraction of stormwater co[r] ...
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In-Vitro Anti-inflammatory Activity Evaluation of the Latex Protease of Holostemma Ada-Kodien Schult
... Inflammation may be acute or chronic. Acute inflammation is characterized by heat, erythema, pain, swelling and loss of function. Pain is a common feature of many diseases and analgesics relieve pain by the action of ...
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Microarray-based component-resolved diagnosis of latex allergy: isolated IgE-mediated sensitization to latexprofilin Hev b8 may act as confounder
... with latex-sensitization as demon- strated by IgE (ImmunoCAP and/or ISAC, 26f/15 m, average age 38 years) only 14 patients reported relevant clinical symptoms upon direct contact with latex and where ...
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Preparation and characterisation of poly (vinyl alcohol)/chitosan/silicon dioxide (PVA/CS/SiO2) beads for the chromium (VI) (Cr (VI)) removal
... polymeric beads can effectively trap many of the ubiquitous organic pollutants, namely, phenolic compounds (Abburi, 2003 & Otero et ...polymeric beads toward other pollutants such as highly ...
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Monocyte subsets differentially employ CCR2, CCR5, and CX3CR1 to accumulate within atherosclerotic plaques
... by flow cytometry, compared with the expected/known frequency (white bars) at which they would enter the peritoneum as unlabeled cells (“expected” data are derived from previously published calculations [ref. 16], as ...
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Intraoperative Anaphylaxis to Latex
... All patients with a history of latex allergy or a history of positive latex RAST or skin test are labeled “LATEX ALLERGY.” All children with my- elomeningocele who are 12 months of age o[r] ...
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A Novel Inherently Radiopaque Bead for Transarterial Embolization to Treat Liver Cancer - A Pre-clinical Study
... sedimented beads were washed 3x with 10 mL deionized water and placed on a shaker plate at 250 rpm for 5 minutes in order to remove residual salts present in the packing solution (beads are supplied in ...
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Newer advances in rubber dam
... New advances in rubber dam sheet Hygenic dental dam Fig1.1 is a non-latex latex rubber dam for patients with latex allergies.. It is a powder-free, free, synthetic dam and comes in just [r] ...
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