LC3-I
Original Article Bortezomib induced autophagy to protect esophageal cancer cells from apoptosis through endoplasmic reticulum stress-mediated way
... Figure 4. BZ induces autophagy through ER stress-mediated way in EC cells. EC cells were treated with 4-PBA, BZ, BZ + 4-PBA, respectively, with non-treated cells as control. A, B. Relative expression of GRP78 and PERK ...
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Autophagy favors Brucella melitensis survival in infected macrophages
... of LC3-II to LC3-I has been used to track changes in autophagic ...of LC3-II to LC3-I under 16M infection and rapa ...of LC3-II to LC3-I in both ...
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Mature adipocytes in bone marrow protect myeloma cells against chemotherapy through autophagy activation
... the effects of adipocytes on Jak/Stat3 activation, we added adipocyte-conditioned media, leptin, or adipsin to cultures of ARP-1 and U266 cells. No addition of adipocyte- conditioned media (Medium) served as a control. ...
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<p>LncRNA SNHG11 Promotes Proliferation, Migration, Apoptosis, and Autophagy by Regulating hsa-miR-184/AGO2 in HCC</p>
... Beclin-1, LC3 II/LC3 I, cleaved Caspase3 in Hep G2 cells transfected with sh- SNHG11, sh-SNHG11+ miR-184 inhibitor, miR-184 inhibitor, sh-AGO2, and negative control; these effects revealed that ...
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Autophagy Benefits the Replication of Newcastle Disease Virus in Chicken Cells and Tissues
... Animal experiments. One-day-old SPF chickens were obtained from Merial (Merial Vital Laboratory Animal Technology Company, Beijing, China) and kept under a controlled temperature (28 to 30°C). The chick- ens were housed ...
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<p>Overexpression of microRNA-216a inhibits autophagy by targeting regulated MAP1S in colorectal cancer</p>
... as LC3, Beclin-I, N-cadherin, E-cadherin, and Vimentin, was eval- uated by Western ...of LC3-II, Beclin-I, N-cadherin, and Vimentin expression; and higher E-cadherin expression were detected ...
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Influenza A Virus Proteins NS1 and Hemagglutinin Along with M2 Are Involved in Stimulation of Autophagy in Infected Cells
... intracellular LC3- I/LC3-II profiles were identified by ...on LC3-II formation during 27-h (not shown) and 40-h ...the LC3-I/LC3-II ra- tios (where the content of ...
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Suppression of mTOR pathway and induction of autophagy- dependent cell death by cabergoline
... Cabergoline (CAB), the first-line drug for treatment of prolactinomas, is effective in suppressing prolactin hypersecretion, reducing tumor size, and restoring gonadal function. However, mechanisms for CAB-mediated tumor ...
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Modification of Cellular Autophagy Protein LC3 by Poliovirus
... of LC3 protein during poliovirus ...of LC3 processing as it occurs during autophagy activation. LC3-I is the unmodified, cleaved form of LC3, while LC3-II is the lipid-conjugated ...
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AEG-1 is involved in hypoxia-induced autophagy and decreases chemosensitivity in T-cell lymphoma
... In addition, we also proposed a mechanism by which AEG-1 enhanced chemoresistance of Hut-78 cells in hypoxia. A large amount of studies have demonstrated that autophagy plays a vital role in hypoxia-induced drug ...
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<p>Long Noncoding RNA HCP5 Regulates Pancreatic Cancer Gemcitabine (GEM) Resistance By Sponging Hsa-miR-214-3p To Target HDGF</p>
... Beclin1, LC3 I/ LC3 II, while miR-214-3p inhibitor signi fi cantly increased p62 and decreased Beclin1, LC3 I/LC3 ...Beclin1, LC3 I/LC3 II induced by the ...
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CDC20 regulates cardiac hypertrophy via targeting LC3-dependent autophagy
... lysosomes, LC3-II on the cytosolic face of autophagosomes is delipidated by ATG4B to form LC3-I, and LC3-II in the autolysosomal lumen is degraded by lysosomal ...Thus, LC3-II is a ...
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MicroRNA 200c-3p regulates autophagy via upregulation of endoplasmic reticulum stress in PC-3 cells
... on LC3-II and Beclin mRNA levels, which are markers of ...of LC3-II and Beclin, while the miR-200c-3p inhibitor attenuated their expression ...of LC3-I to LC3-II following overex- ...
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<p>Induction of Autophagy by Baicalin Through the AMPK-mTOR Pathway Protects Human Skin Fibroblasts from Ultraviolet B Radiation-Induced Apoptosis</p>
... of LC3-II/LC3-I, and the expression of beclin-1 (Figure 5B, C, and D) were all downregulated, suggesting the inhibition of AMPK could suppress the effect of baicalin on UVB-induced autophagy in ...
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Original Article Epidermal growth factor protects against ultraviolet damage in human corneal epithelial cells through inhibiting autophagy
... of LC3-I and LC3-II by Western ...of LC3-I and LC3-II compared to UV+EGF group, suggesting that inhibition of ROS could enhance cell autophagy (Figure ...
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LC3, an autophagosome marker, is expressed on oligodendrocytes in Nasu-Hakola disease brains
... both LC3-I, the soluble cytosolic form and LC3-II, the autophagy-inducible phosphati- dylethanolamine (PE)-conjugated form (Additional file 1: Figure S1a, b, lanes 1, ...of LC3 in the serial ...
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Modulation of Mitochondrial Function and Autophagy Mediates Carnosine Neuroprotection Against Ischemic Brain Damage
... of LC3-II from LC3-I (B) and the phosphorylation of mTOR (C) in NMDA (25 μM)-stimulated primary neurons with or without carnosine (100 μM) ...
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Induction of apoptosis and autophagy via mitochondria- and PI3K/Akt/mTOR-mediated pathways by E. adenophorum in hepatocytes of saanen goat
... punctate LC3, the ratio of LC3-II/LC3-I and the protein levels of Beclin 1, but decreased that of P62, indicating the potent pro-autophagic effects of ...
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Improved efficacy of a next-generation ERT in murine Pompe disease
... Lamp1, LC3-II, and the autopha- gy-specific substrate SQSTM1/p62 compared with those after alglucosidase alfa administration (Figure 4, ...B–E). LC3 exists as a soluble form, LC3-I, and an ...
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Vital Role for CD8+ Cells in Controlling Retroviral Infections
... of I/LnJ mice infected with different virus ...in I/LnJ mice infected with the low-titer variant MMTV(C3H) prevent viral progression from the lymphoid system to the mammary glands, while in I/LnJ ...
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