lentiviral vectors
Systemic epigenetic response to recombinant lentiviral vectors independent of proviral integration
... derived lentiviral vectors (LV) expressing eGFP under the control of the human PGK promoter and pseu- dotyped with VSVg using a 3- or 4-plasmid system as indicated in Additional file 1 were produced by ...
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Mobilization and Mechanism of Transcription of Integrated Self-Inactivating Lentiviral Vectors
... and lentiviral vectors have a predilection for integration into transcriptionally active genes, creating the potential for promoter activation or gene disrup- ...(SIN) vectors in which a deletion of ...
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Selection of Novel Vesicular Stomatitis Virus Glycoprotein Variants from a Peptide Insertion Library for Enhanced Purification of Retroviral and Lentiviral Vectors
... successfully pseudotype lentiviral vectors when packaged at 37°C, though titers were low. It is important to note that two of the novel sites that are identified in this work flank this ratio- nally ...
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Engineering fusogenic molecules to achieve targeted transduction of enveloped lentiviral vectors
... of two domains; E1 is responsible for mediating the fusion between the virus and target cell and E2 is respon- sible for directing the binding of the virus to the cellular antigen on the target cell surface [28]. Chen ...
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Transduction with Lentiviral Vectors Altered the Expression Profile of Host MicroRNAs
... (shRNA) vectors are more widely ...with lentiviral shRNA vectors inhibited hepatitis C virus (HCV) propagation in human hepatoma ...The lentiviral vector itself and the integration site of the ...
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Effective in vivo and ex vivogene transfer to intestinal mucosa by VSV-G-pseudotyped lentiviral vectors
... Methods: We evaluated vesicular stomatitis virus glycoprotein envelope (VSV-G)-pseudotyped lentiviral vectors (LV) for efficacy of gene transfer to both murine rectosigmoid colon in vivo and human colon ...
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Effect of the Internal Promoter on Insertional Gene Activation by Lentiviral Vectors with an Intact HIV Long Terminal Repeat
... gammaretroviral vectors caused leukemias in 5 out of 20 patients treated for X-linked severe combined immune deficiency in Paris and London (6, ...and lentiviral vectors were tested and found to gen- ...
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Vpu dependent block to incorporation of GaLV Env into lentiviral vectors
... between lentiviral vectors and GaLV ...expressed, lentiviral vectors resulting from the co-expression of GaLV Env and ...into lentiviral vectors based on simian immunodeficiency ...
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Measles Virus Glycoprotein-Pseudotyped Lentiviral Vectors Are Highly Superior to Vesicular Stomatitis Virus G Pseudotypes for Genetic Modification of Monocyte-Derived Dendritic Cells
... viral vectors (8, 22, 66, 71, 89). The most attractive vectors capable of introducing a transgene into DCs are adenoviral vec- tors (50, 77), gamma retroviral vectors (84), and lentiviral ...
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Transcriptional Targeting of Lentiviral Vectors by Long Terminal Repeat Enhancer Replacement
... targeted lentiviral vectors by replacing the viral LTR U3 enhancer ele- ments with a DNase hypersensitive region (HS2) upstream of the erythroid-specific, GATA-1 zinc finger transcription factor gene (8, ...
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Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI
... [12,13] vectors to construct multigene vectors. Multigene lentiviral vectors have been developed by other groups using strategies involving inclusion of IRES [14], multiple internal promoters ...
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In Vivo Assessment of Gene Delivery to Keratinocytes by Lentiviral Vectors
... production. Lentiviral vectors that express GFP driven by a cytomeg- alovirus (CMV) promoter were produced by transient transfection of the fol- lowing plasmids into 293T cells: a packaging plasmid ...
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HIV-Derived Lentiviral Vectors: Current Progress toward Gene Therapy and DNA Vaccination
... Lentiviral vectors are promising gene delivery tools capable of transducing a variety of dividing and non-dividing cells, including pluripotent stem cells which are refractory for transduction by murine ...
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Redirecting Lentiviral Vectors Pseudotyped with Sindbis Virus-Derived Envelope Proteins to DC-SIGN by Modification of N-Linked Glycans of Envelope Proteins
... of lentiviral vectors, was purchased from Addgene (Cambridge, ...self-inactivating lentiviral vector containing the MLV (murine leukemia virus) promoter as its internal promoter (79), was used for ...
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Cassette deletion in multiple shRNA lentiviral vectors for HIV 1 and its impact on treatment success
... per infection [44]). This implies that every HIV-1 DNA is recombinant, though recombination will only produce a change if a cell is multiply infected, which is rarer. Previ- ous studies of different double repeats have ...
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Hemophilia A gene therapy via intraosseous delivery of factor VIII-lentiviral vectors
... desired. Lentiviral vector (LV)-mediated gene transfer into hematopoietic stem cells (HSCs) results in stable integration of FVIII gene into the host genome, leading to persistent therapeutic ...
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Efficient Functional Pseudotyping of Oncoretroviral and Lentiviral Vectors by Venezuelan Equine Encephalitis Virus Envelope Proteins
... The identities of the receptors for all alphaviruses still re- main ambiguous, although reports have indicated roles for the laminin receptor (32), glycosaminoglycans (15), and DC-SIGN in aiding infection (13). The ...
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Efficient and Highly Specific Gene Transfer Using Mutated Lentiviral Vectors Redirected with Bispecific Antibodies
... (LV) vectors limits their use for targeted gene delivery in ...the vectors with a bispecific antibody (bsAb) that binds both LV and receptors on target ...
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Engineering lentiviral vectors for modulation of dendritic cell apoptotic pathways
... In this report, we also confirmed that LV transduction (using one of our novel vectors specifically) did not alter the morphology, phenotype, or function of DC2.4s and BMDCs, consistent with findings from our ...
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Delivery of Factor VIII Gene into Skeletal Muscle Cells Using Lentiviral Vector
... viral vectors, adeno-associated viral vectors, retroviral vectors and lentiviral ...drawbacks. Lentiviral-based vectors present several attractive features for FVIII gene ...
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