leptomycin B (LMB)
The effects of leptomycin B on HPV infected cells
... control. Leptomycin B (LMB), a nuclear export inhibitor, has previously been shown to induce apoptosis in HPV-containing cancer cell lines and HPV 16 E7 or E6/E7 transduced primary keratinocytes, but ...
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Role of Immediate Early Protein ICP27 in the Differential Sensitivity of Herpes Simplex Viruses 1 and 2 to Leptomycin B
... Leptomycin B (LMB) is a highly specific inhibitor of CRM1, a cellular karyopherin- that transports nuclear export signal-con- taining proteins from the nucleus to the ...that LMB blocks ...
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Leptomycin B Inhibits Equine Infectious Anemia Virus Rev and Feline Immunodeficiency Virus Rev Function but Not the Function of the Hepatitis B Virus Posttranscriptional Regulatory Element
... by leptomycin B, a drug that specifically blocks NES-CRM1 ...hepatitis B virus (HBV) posttranscrip- tional regulatory element (PRE)-containing and Mason-Pfizer monkey virus (MPMV) constitutive ...
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A Single Amino Acid Substitution in the ICP27 Protein of Herpes Simplex Virus Type 1 Is Responsible for Its Resistance to Leptomycin B
... 10 g of actinomycin D per ml (E) were fixed. The nonspecific binding of antibodies was blocked by preincubation with human serum (22). Cells were treated with RNase in order not to permit the binding of propidium iodide ...
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Cancerous multi drug resistance is reduced by Leptomycin B treatment in CCRF CEM/Taxol cellsCancerous multi drug resistance is reduced by Leptomycin B treatment in CCRF CEM/Taxol cells
... of LMB, CCRF-CEM/ Taxol and CCRF-CEM cells were treated with different concentration of ...of LMB was observed after treating the cells with LMB for 48 hrs (Figure ...1(A)). LMB reduced the ...
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Trafficking of some old world primate TRIM5α proteins through the nucleus
... Localization of TRIM5 variants was studied as pre- viously described [60]. Briefly, cells were grown over- night on 12-mm-diameter coverslips and fixed in 3.9% paraformaldehyde (Sigma) in phosphate-buffered saline (PBS; ...
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Direct Evidence from Single-Cell Analysis that Human α-Defensins Block Adenovirus Uncoating To Neutralize Infection
... hibitor leptomycin B ...of LMB reduced the nuclear localization of hexon without affecting the extent of uncoating ...and B). The modest quantitative effect of LMB on nuclear ...
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CRM1-Dependent Transport Supports Cytoplasmic Accumulation of Adenoviral Early Transcripts
... of the cells used in the present study. HA-CANc cells were treated with 1 g of tetracycline/ml. A549 cells were treated with 20 nM leptomycin B (LMB). Living cells were counted at indicated times ...
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Regulation of Stat3 nuclear export
... toxin leptomycin B ...Stat1, LMB treatment not only blocks the poststimulation export of Stat3 from the nucleus back to the cytoplasm, but also promotes the nuclear accumulation of Stat3 in resting ...
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Importin-β Family Members Mediate Alpharetrovirus Gag Nuclear Entry via Interactions with Matrix and Nucleocapsid
... Identification of importin-  family members utilized for import of MA and NC. We undertook these studies to identify both cis and trans elements involved in the transport of RSV Gag proteins into the nucleus. Based on ...
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LEDGF Dominant Interference Proteins Demonstrate Prenuclear Exposure of HIV-1 Integrase and Synergize with LEDGF Depletion To Destroy Viral Infectivity
... dependency factor’s role in the viral life cycle is lacking. Co- factor function requires intact NDE and IBD attachment func- tions (30, 46). DI protein experiments have also shown that uncoupling these functions can ...
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Adenovirus E1B 55-Kilodalton Protein Is a p53-SUMO1 E3 Ligase That Represses p53 and Stimulates Its Nuclear Export through Interactions with Promyelocytic Leukemia Nuclear Bodies
... A key observation for understanding the mechanism by which E1B-55K inhibits p53 function is that mutation of the consensus sumoylation site in the E1B-55K K104R mutant greatly interferes with E1B-55K inhibition of p53 ...
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Functions and regulation of Hdmx and post-translational modifications in drug sensitivity and cancer.
... We have found that the treatment of several types of cells with Leptomycin B, which blocks nuclear export of proteins containing a Rev-like nuclear export signal, strongly increases al[r] ...
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Host Cell Nuclear Proteins Are Recruited to Cytoplasmic Vaccinia Virus Replication Complexes
... rich nuclear export signal peptide that mediates nuclear export through the Crm1-dependent pathway (6). The localization of the protein is monitored by green fluorescence microscopy. The fusion protein normally resides ...
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Polyamide 66 microspheres metallised with in situ synthesised gold nanoparticles for a catalytic application
... the coordination with gold complex charged negatively. After reduction of the gold precursor, Au NPs covered the PA66 microsphere surfaces due to the physical interaction formed between both materials. PA66/Au NP hybrid ...
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Characterization of the Nucleocytoplasmic Shuttle of the Matrix Protein of Influenza B Virus
... 15-95 and EGFP-15-123, which contained one or two of the pre- dicted NLSs but none of the predicted NESs, were predominantly located in the nucleus (Fig. 2B). These results suggest that the predicted NLS1(76 to 94 aa) is ...
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Making Sustainable Development Real Through Role-Play: “The Mekong Game” Example
... to LMB mainstream dams, if all were to proceed, are expected to be worth USD 476million/year, excluding effects on the coastal and delta fisheries which are likely to be significant but have not been ...to ...
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Study of Serotypes, Susceptibility to Macrolide and Virulence and Resistance Molecular Profiles in Invasive Strains of Streptococcus agalactiae in Two Argentine Provinces
... group B streptococcal, GBS), initially described by Nocarrd and Mollereau in 1887 [1] was isolated from vaginal cultures in 1935, was listed as a human pathogen for the first time in 1938 as cause of fatal ...
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MLV requires Tap/NXF1 dependent pathway to export its unspliced RNA to the cytoplasm and to express both spliced and unspliced RNAs
... Figure 1 Effects of CRM1 and Tap inhibition on MLV expression. (A) RNAs produced by MLV and their encoded proteins. Black arrows correspond to the RT primers while positions of the RT-qPCR primers used to amplify the ...
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Dual B- and T-cell de-immunization of recombinant immunotoxin targeting mesothelin with high cytotoxic activity
... kill B- and T-cells, additional treatment cycles could be given and major tumor responses were observed in several patients with advanced refractory mesothelioma ...
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