Ligase IV
DNA ligase IV syndrome; a review
... Until recently LIG4 syndrome was only identified through genetic testing after the diagnosis of immuno- deficiency and/or malignancy in microcephalic patients. Murray et al. observed this and took the reverse ap- proach ...
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Successful bone marrow transplantation in a patient with DNA ligase IV deficiency and bone marrow failure
... dren with Dubowitz syndrome [14,15] leading to consid- eration of this diagnosis. Our patient increasingly suffered from recurrent infections of the inner ear and respiratory tract with low immunoglobulin levels, ...
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The Drosophila melanogaster DNA Ligase IV Gene Plays a Crucial Role in the Repair of Radiation-Induced DNA Double-Strand Breaks and Acts Synergistically With Rad54
... DNA Ligase IV of the deletions in the Lig4 gene, PCR products were gel in a multicellular organism, we isolated the Drosophila purified, cloned into pGEM-T Easy (Promega, Madison, WI), and ...DNA ...
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Ligase IV inhibitor SCR7 enhances gene editing directed by CRISPR–Cas9 and ssODN in human cancer cells
... DNA ligase IV inhibitor, SCR7, can promote insertion repair efficiency at AAVS1 locus using ssODN as repair templates in MCF-7 and HCT-116 cells, a 96-nucleotide fragment, AAVS1-EcoRI-CRISPR-96, which ...
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End-Joining Repair of Double-Strand Breaks in Drosophila melanogaster Is Largely DNA Ligase IV Independent
... Repair of DNA double-strand breaks can occur by either nonhomologous end joining or homologous recombination. Most nonhomologous end joining requires a specialized ligase, DNA ligase IV (Lig4). In ...
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Deletion of the DNA Ligase IV Gene in Candida glabrata Significantly Increases Gene-Targeting Efficiency
... The process of NHEJ is less complicated in yeasts than in mam- malian cells, where it requires many proteins. In yeast, NHEJ de- pends on the combined action of KU genes and DNA ligase IV (13, 14). ...
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Allogeneic hematopoietic stem cell transplantation in two brothers with DNA ligase IV deficiency: a case report and review of the literature
... percentile), length 49 cm (7th percentile), head circum- ference 33 cm (<3rd percentile)), the first 3 months of his life were uneventful. Subsequently, numerous pul- monary infections and chronic bronchitis with ...
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Adenovirus E4 34k and E1b 55k Oncoproteins Target Host DNA Ligase IV for Proteasomal Degradation
... DNA ligase IV and Mre11 becomes pronounced only fairly late in infection (16 to 24 h; ...ubiquitin ligase and the time at which appreciable degradation of DNA ligase IV and Mre11 occurs ...
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Impaired lymphocyte development and antibody class switching and increased malignancy in a murine model of DNA ligase IV syndrome
... Here, we have demonstrated that the Lig4Y288C mutation leads to multiple defects in lymphocyte development and function, including impaired VDJ recombination, peripheral lymphocyte survi[r] ...
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Serotype-Specific Inactivation of the Cellular DNA Damage Response during Adenovirus Infection
... DNA ligase IV is degraded during infection with all of the viruses examined, probably preventing the ille- gitimate repair of viral genomes by ...DNA ligase IV by immunofluorescence ...
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Nijmegen breakage syndrome (NBS)
... DNA ligase IV deficiency (LIG4 syndrome) and severe combined immunodeficiency with microcephaly, growth retardation, and sensitivity to ionizing radiation due to NHEJ1 deficiency (NHEJ1 ...
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HIV 1 Tat Recruits HDM2 E3 Ligase To Target IRF 1 for Ubiquitination and Proteasomal Degradation
... E3 ligase capable of mediating IRF-1 ubiquitination during virus infection had not been identi- ...E3 ligase recruited to IRF-1 by Tat, we also demonstrate that HDM2 medi- ated K48-linked ubiquitination of ...
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Mammalian DNA ligase II
... A ligase II could be in volved in D N A repair, b u t th ere is no direct experim ental evidence for th is n otion ...A ligase active in replication, c o n stitu tiv e ex cisio n repair, and m ito tic recom ...
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BCHM3050 Lecture 12 F14.ppt
... Dimer Dimer DNA DNA Polymerase I Polymerase I Clamp Clamp Protein Protein Leading Leading Strand Strand Lagging Lagging Strand Strand Primosome Primosome DNA Ligase DNA Ligase Replicatio[r] ...
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Comparative efficacy of IV phenytoin, IV valproate and IV levetiracetam in childhood seizures
... valproate loading. To the best of our knowledge no such comparative study was available for IV levetiracetam. Out of all patients in the three study groups only one patient from valproate group had a repeat ...
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POLYNUCLEOTIDE LIGASE IN BACTERIOPHAGE T4D RECOMBINATION
... The experiments on the time course of recombinant formation following infection with wild type, lysis-inhibited wild type, or gene t mutants indicate that under standard cross cond[r] ...
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Synthesis, Characterization and Antifungal Activity of 3 Substituted Triazipino [3,4 b] [1,3] Benzothiazole
... Two moieties are fused and screened for antifungal studies they showed a broad spectrum of antifungal activity. They showed good activity against Penicillium sp and Aspergillus Niger species. ...
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The E3 ligase Cdh1 anaphase promoting complex operates upstream of the E3 ligase Smurf1 in the control of axon growth
... In axon growth assays using granule neurons, we analyzed control neurons, Smurf1 knockdown neurons and Smurf1 knockdown neurons that express Smurf1-Res, and found that Smurf1-Res restore[r] ...
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“SYNTHESIS AND ANTIMICROBIAL ACTIVITY OF NOVEL 3-CHLORO- 4-(SUBSTITUTED ARYL)-1,3DIPHENYL-1H-PYRAZOLE-4-YL-1-(6-METHYLPYRIDIN-2-YL) AZETIDIN-2-ONE DERIVATIVES” by Dr Jabali J. Vora, *Akash R, Patel, Dr Dinesh R. Patel, Yamini S. Patel, India.
... Coli: Compounds no IV-b, IV-d, IV-i, IV-j, IV-k and IV-m possess higher or equal antibacterial activity against E.Coli compared to standard antibiotic ampicillin a[r] ...
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Transfection of Escherichia coli Spheroplasts IV. Transfection of rec+ and rec− Spheroplasts by Native, Denatured, and Renatured T5 Bacteriophage DNA After Repair of Single-Strand Breaks by Polynucleotide Ligase
... One possible explanation for the two-hit nature of the plot of infective centers versus DNA concentration is the extreme shear sensitivity of T5 phage DNA 1, 13; if the DNA were broken i[r] ...
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