lightcycler pcr
Development of a LightCycler PCR Assay for Detection and Quantification of Aspergillus fumigatus DNA in Clinical Samples from Neutropenic Patients
... LightCycler PCR-based quantification of Aspergillus DNA in clinical ...nested PCR assay described previously (47), we investigated 10 BAL samples from nine neutropenic patients with hematolog- ical ...
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Comparison of LightCycler PCR, Rapid Antigen Immunoassay, and Culture for Detection of Group A Streptococci from Throat Swabs
... Cycler PCR (10 min versus ...the LightCycler PCR assay (including the ...the LightCycler PCR method or ...with LightCycler PCR technology, is that low colony counts for ...
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Diagnosis of Herpes Simplex Virus Infections in the Clinical Laboratory by LightCycler PCR
... of PCR detection of amplified HSV DNA prod- ucts is highly relevant for clinical microbiology practice, espe- cially for the laboratory diagnosis of the infections involved in sexually transmitted ...and ...
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Direct and Rapid Identification and Genogrouping of Meningococci and porA Amplification by LightCycler PCR
... Invasive meningococcal infections are usually diagnosed by culture. This approach is far from optimal due to, e.g., treatment with precollection antibiotics. Molecular-genetics methods are therefore recognized as ...
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Multiplex LightCycler PCR Assay for Detection and Differentiation of Bordetella pertussis and Bordetella parapertussis in Nasopharyngeal Specimens
... multiplex PCR assay for detecting and differentiating Bordetella pertussis and Bordetella parapertussis in nasopharyngeal swabs was ...the LightCycler (Roche Molecular Biochemicals, Indianapolis, ...second ...
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Sensitive Detection of Borrelia burgdorferi Sensu Lato DNA and Differentiation ofBorrelia Species by LightCycler PCR
... in LightCycler PCR, to our knowledge) has a wide heterogeneity in the three species and can be amplified in two steps with nested primers (14, ...published PCR protocol yielded greater sensitivities ...
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Detection and Differentiation of Human Polyomaviruses JC and BK by LightCycler PCR
... by PCR is recognized as a sensitive and specific method for detecting human polyomaviruses in clinical ...real-time PCR assay using the LightCycler platform was evaluated and compared to an ...
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Detection of Smallpox Virus DNA by LightCycler PCR
... Activation of bioterrorism response plans depends on the immediate clinical suspicion and recognition of the disease; this step must be followed by the rapid and accurate laboratory diagnosis of the smallpox virus ...
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Rapid Detection and Molecular Differentiation of Toxigenic Corynebacterium diphtheriae and Corynebacterium ulcerans Strains by LightCycler PCR
... real-time PCR concepts, i.e., TaqMan and LightCycler PCR, reliable protocols for both detection systems are ...a LightCycler PCR assay which reliably detects all human-derived ...tox ...
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Detection of Bacillus anthracis DNA by LightCycler PCR
... conventional PCR testing has not been widely imple- mented in clinical laboratories, in part due to its requirement for time-consuming and labor-intensive steps, which include specimen preparation, amplification, ...
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Comparison of the Roche LightCycler vanA/vanB Detection Assay and Culture for Detection of Vancomycin Resistant Enterococci from Perianal Swabs
... the LightCycler vanA/vanB detection assay. The LightCycler assay was performed on bacterial colonies growing on blood agar ...multiplex PCR-restriction fragment length polymor- phism (RFLP) assay ...
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Use of the Roche LightCycler Strep B Assay for Detection of Group B Streptococcus from Vaginal and Rectal Swabs
... the LightCycler Strep B assay. The analytical specificity of the LightCycler PCR assay was determined by evaluation of isolated colonies of ...extracts, PCR with broad- range 16S rRNA primers ...
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Rapid Diagnosis of Bacterial Meningitis by Real Time PCR and Fluorescence In Situ Hybridization
... For PCR analysis, a real-time LightCycler approach was chosen to maximize the speed of ...broad-range PCR assay, based on a previously published primer pair (5), was adapted to a real-time ...
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Multilocus Sequence Typing of Methicillin Resistant Staphylococcus aureus from an Area of Low Endemicity by Real Time PCR
... System PCR for MLST offers the advantage to perform efficient and rapid amplification, and real-time detec- tion of the seven housekeeping genes in the same PCR ...the LightCycler System PCR ...
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Comparison of Quantitative Competitive PCR with LightCycler Based PCR for Measuring Epstein Barr Virus DNA Load in Clinical Specimens
... by LightCycler PCR using standard curves generated either in water or in a DNA back- ground equivalent to 5 l of blood from EBV DNA-negative healthy ...
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Direct Detection of Rifampin and Isoniazid Resistant Mycobacterium tuberculosis in Auramine Rhodamine Positive Sputum Specimens by Real Time PCR
... by LightCycler PCR when all pair of probes were ...other PCR assays, and the AR counts in the smear were one to nine per field (at a magnification of ⫻400) or more in four of them and one to nine per ...
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Rapid and Sensitive Detection of Single Cryptosporidium Oocysts from Archived Glass Slides
... real-time LightCycler PCR detection of 18S rRNA, which produced 232-bp PCR ...of PCR detection of ...232-bp PCR products from 18S ...sequencing LightCycler PCR ...
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Development of Real Time PCR Assays and Evaluation of Their Potential Use for Rapid Detection of Burkholderia pseudomallei in Clinical Blood Specimens
... real-time PCR assays targeting novel genetic DNA markers that are unique to ...real-time PCR assays can be used for the rapid and specific verification of ...real-time PCR assays developed in this ...
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A Comparison of the Clinical and Molecular Diagnosis of Herpes Simplex Keratitis
... of PCR negative results for HSV1 in samples clinically di- agnosed as typical ...time PCR to achieve a concentration similar to that found in HSV1 PCR positive clinical samples (cycle threshold 22) ...
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Diagnosis of Prosthetic Joint Infection by Use of PCR Electrospray Ionization Mass Spectrometry
... 19. Portillo ME, Salvado M, Sorli L, Alier A, Martinez S, Trampuz A, Gomez J, Puig L, Horcajada JP. 2012. Multiplex PCR of sonication fluid accurately differentiates between prosthetic joint infection and aseptic ...
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