Limited Proteolysis
Probing Conformational Feature of a Recombinant Pyruvate Kinase by Limited Proteolysis
... that limited proteolysis experiments can be successfully used to probe conformational features of proteins, in this study we obtained useful information on Geobacillus pyruvate kinase using limited ...
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A new fibrinogenase from Echis multisquamatis venom is a perspective agent for limited proteolysis and defibrinogenation
... A serine proteinase with a fibrinogenase activity was isolated from the venom of viper Echis multisquama- tis. Isolation was performed by the combination of Q-sepharose and Heparine-agarose chromatography. The enzyme has ...
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A comparison of the folding characteristics of free and ribosome-tethered polypeptide chains using limited proteolysis and mass spectrometry.
... By using the SH3/SH3-m10 system, which has been studied previously at the atomic level using NMR 7 , as proof of principle we have demonstrated the utility of limited proteolysis coupled with MS to report ...
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Scrapie prion rod formation in vitro requires both detergent extraction and limited proteolysis.
... To determine whether both detergent extraction and limited proteolysis are required for the formation of prion rods, microsomal membranes were prepared from infected brains in the presen[r] ...
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Comparisons of rotavirus polypeptides by limited proteolysis: close similarity of certain polypeptides of different strains.
... To investigate the assumed identities of proteins observed in virusinfected cells and in purified virus based on electrophoretic mobilities in SDS-polyacrylamide gels, the limited proteo[r] ...
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Human neutrophil elastase modulates platelet function by limited proteolysis of membrane glycoproteins
... We conclude that elastase inhibition of thrombin-induced platelet stimulation is accompanied by a a reduction in the number of thrombin binding sites per platelet and b proteolysis of gl[r] ...
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Effect of monoclonal antibodies on limited proteolysis of native glycoprotein gD of herpes simplex virus type 1.
... Several criteria were used to distinguish the different antigenic determinants of gD which were recognized by these antibodies, including: i neutralization of HSV-1 and HSV-2 infectivity[r] ...
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Limited proteolysis of herpes simplex virus glycoproteins that occurs during their extraction from vero cells.
... In the absence of appropriate protease inhibitors, both mature and immature forms of four different glycoproteins specified by herpes simplex virus type 2 were significantly smaller base[r] ...
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Regulation of limited N-terminal proteolysis of APE1 in tumor via acetylation and its role in cell proliferation
... tumor or tumor-adjacent non-tumor tissue extract. Two truncated forms of APE1 were produced in the absence of protease inhibitors (Figure 3A, left panel), and were identical to the APE1 isoforms observed in the patient ...
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Globular domain structure and function of restriction-like-endonuclease LINEs: similarities to eukaryotic splicing factor Prp8
... Using limited proteolysis as a probe for globular domain structure, we show that the protein encoded by the Bombyx mori R2 element has two major globular domains: (1) a small globular domain consisting of ...
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Pocket Factors Are Unlikely To Play a Major Role in the Life Cycle of Human Rhinovirus
... Infectivity, limited proteolysis, and matrix-assisted laser desorption ionization analyses indicate that filling the drug-binding pocket with bulky side chains is not deleterious to the viral life cycle and ...
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A vitellogenin polyserine cleavage site: highly disordered conformation protected from proteolysis by phosphorylation
... to proteolysis and ...used limited proteolysis to see whether (a) the natural AmVg cleavage pattern can be triggered with trypsin/chymotrypsin and (b) the digestion pattern matches the expectations ...
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A tale of a tail: Structural insights into the conformational properties of the polyglutamine protein ataxin-3
... of limited proteolysis and ESI-IMS–MS experiments we provide insights into the confor- mational properties of the non-expanded ataxin-3 (14Q), which contains a stretch of thirteen glutamine residues ...
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Targeted Deletion of MIC5 Enhances Trimming Proteolysis of Toxoplasma Invasion Proteins
... Limited proteolysis of proteins transiently expressed on the surface of the opportunistic pathogen Toxoplasma gondii accompanies cell invasion and facilitates parasite migration across cell barriers during ...
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Comparative Analysis of Adeno-Associated Virus Capsid Stability and Dynamics
... the limited-proteolysis experiments conducted at ...in limited-proteolysis experiments, the rel- atively low sequence specificity of proteinase K minimizes se- quence bias, emphasizing ...
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Proteolysis of Myosin during Platelet Storage
... Physical properties of actomyosin from either fresh or stored platelets have been compared. Actomyosin obtained from platelets after 3 days of storage contained myosin that was 60- 80% degraded to myosin rod. No myosin ...
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The Surface Conformation of Sindbis Virus Glycoproteins E1 and E2 at Neutral and Low pH, as Determined by Mass Spectrometry-Based Mapping
... used limited proteolysis in combination with MS to determine which amino acids are accessible on the surface of the glycoprotein spike complex and how the confor- mation of these protein domains change when ...
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Sjuts, Inga (2018): Topology and function of the inner envelope protein Tic110. Dissertation, LMU München: Fakultät für Biologie
... From limited proteolysis using isolated inner envelope vesicles it could be concluded that Tic110 exposes domains which are found in the intermembrane space of ch[r] ...
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Effects of Different RNAs and Components of the Cell-Free System on In Vitro Synthesis of Sindbis Viral Proteins
... We were reluctant to conclude that proteolysis was occuring so well in vitro because i the ascites cell-free system fails to show a proteolysis of the polypeptides translated from enceph[r] ...
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Ubiquitin Like Proteasome System Represents a Eukaryotic Like Pathway for Targeted Proteolysis in Archaea
... SPS-targeted proteolysis of TBP2 by removing SAMP2, which could otherwise sterically block the translocation of the substrate into the proteolytic chamber of proteasomal ...mediated proteolysis, Anjum et ...
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