limiting dilution
A high throughput method to detect Plasmodium falciparum clones in limiting dilution microplates
... Sensitivity and specificity of the c-SNARF-1 assay were compared to microscopy of Giemsa-stained smears with limiting dilution experiments using eight microplates. From these plates, 39/480 wells were ...
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An improved method for undertaking limiting dilution assays for in vitro cloning of Plasmodium falciparum parasites
... Histidine-rich protein II (HRP2) is a water-soluble non- labile protein produced by P. falciparum across the asexual cycle and is secreted into parasite culture media where it can be detected by an antigen capture ELISA. ...
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Comparison of Parasite Burden Using Real-Time Polymerase Chain Reaction Assay and Limiting Dilution Assay in Leishma-nia major Infected Mouse
... of growth. To compare parasite burdens obtained by real-time PCR assay with those of limiting dilution assay, twelve 8-fold serial dilutions of the lymph node homogenates were prepared in the Schneider ...
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Comparison of plasma virus loads among individuals infected with hepatitis C virus (HCV) genotypes 1, 2, and 3 by quantiplex HCV RNA assay versions 1 and 2, Roche Monitor assay, and an in house limiting dilution method
... The accuracy of different methods for the quantitation of hepatitis C virus in plasma was measured with samples from individuals infected with different genotypes and by using RNA transcripts of predetermined ...
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Array-Based Transcript Profiling and Limiting-Dilution Reverse Transcription-PCR Analysis Identify Additional Latent Genes in Kaposi's Sarcoma-Associated Herpesvirus
... Kaposi’s sarcoma-associated herpesvirus (KSHV) is a B-lymphotropic herpesvirus strongly linked to both lymphoproliferative diseases and Kaposi’s sarcoma. The viral latency program of KSHV is central to persistent ...
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Carboxyl terminal and central regions of human immunodeficiency virus 1 NEF recognized by cytotoxic T lymphocytes from lymphoid organs An in vitro limiting dilution analysis
... specific CD8+ lymphocytes. In the present report, we show that HIV-specific CTL responses can also be detected in lymph nodes and spleens, and were mainly directed against the ENV, GAG, and NEF HIV-1 proteins. The ...
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Investigation into limiting dilution and tick transmissibility phenotypes associated with attenuation of the S24 vaccine strain
... For cloning by limiting dilution, fresh B. bovis S17.2 para- site infected blood was collected at the peak of the ani- mal’s reaction. The number of parasites per ml blood was determined (percentage of ...
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Specificity of human natural killer cells in limiting dilution culture for determinants of herpes simplex virus type 1 glycoproteins.
... To examine whether T-cell-depleted effector cells grown in LD culture showed cytotoxicity characteristic of NK cells, LD cultures were also tested against K562 target cells, the cell lin[r] ...
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Genotype Dependence of Hepatitis C Virus Load Measurement in Commercially Available Quantitative Assays
... Samples. Ninety-six plasma samples from HCV-infected, PCR-positive blood donors (20 samples each of genotypes 1, 2, and 3; 19 of genotype 4; 1 of genotype 5; and 16 of genotype 6) were analyzed for HCV RNA levels. Type 4 ...
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Specificities and sensitivities of three systems for determination of antibodies to human immunodeficiency virus by electrophoretic immunoblotting
... We therefore tried to inhibit either the binding of a limiting dilution of rabbit anti-HLA class I and II sera with a large excess of three different human anti-HIV sera or the binding o[r] ...
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Improved Assessment of T-Cell Receptor (TCR) VB Repertoire in Clinical Specimens: Combination of TCR-CDR3 Spectratyping with Flow Cytometry-Based TCR VB Frequency Analysis
... using limiting dilution, the enzyme-linked immunospot assay, ex vivo TCR variable (v)-segment analysis determined by flow cytometry, and TCR-CDR3 length analysis (spectratyping), as well as identifica- tion ...
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Two Neutralizing Human Anti-RSV Antibodies: Cloning, Expression, and Characterization
... Limiting Dilution Cloning of EBV-Transformed Cells To assure monoclonality and simplify gene clon- ing, the EBV-transformed cells producing RF-1 and RF-2 were single-cell cloned by limit[r] ...
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Tumor-initiating cell frequency is relevant for glioblastoma aggressiveness
... We demonstrated that all GBMs analysed in this study contain rare TICs sustaining tumor growth, in accordance with other studies in GBMs and other types of tumors [5, 7, 12, 15, 17, 18]. Our results clearly show that not ...
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Host-Range Restrictions of Murine Leukemia Viruses in Mouse Embryo Cell Cultures
... Attempts to resolve possible mixtures by two limiting dilution passages in sensitive cells did not alter the host-range characteristics; i.e., Moloney virus remained NB-tropic and repres[r] ...
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Engineering of isogenic cells deficient for MR1 with a CRISPR/Cas9 lentiviral system: tools to study microbial antigen processing and presentation to human MR1-restricted T cells
... FIGURE 2. Characterization of A549 clonal derivatives bearing biallelic disruptive mutations in the MR1 gene. ( A ) Flow cytometry analysis of MR1 expression in eight different A549 clones obtained by limiting ...
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Monoclonal antibodies to P24 and P61 immunodominant antigens from Nocardia brasiliensis.
... weight bands varied from 12 to 100 and were used in the Western blot assays to study the specificity of isolated MAbs. Figure 2 shows the SDS-PAGE gradient gel, which demon- strates that the major component of this ...
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Multipotency of mouse trophoblast stem cells
... In vitro, CD117 + TSCs were cultured, at a limiting dilution in growth medium for the development of multicellular clones and in specialized medium for differentiation into lung epitheli[r] ...
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Resident macrophages influence stem cell activity in the mammary gland
... were transplanted in a volume of 10 μl into the de-epithelial- ized fourth mammary fat pads of three week-old syngeneic mice. Limiting dilution and statistical analyses were carried out as described by ...
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Characterization of Human Immunodeficiency Virus Type 1 (HIV-1) Gag- and Gag Peptide-Specific CD4+ T-Cell Clones from an HIV-1-Seronegative Donor following In Vitro Immunization
... In order to identify the peptides specifically recognized by the 6 Gag peptide-specific limiting-dilution CD4⫹ T-cell clones, proliferation assays were performed in which each clone was [r] ...
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Ex-vivo expansion of nonhuman primate CD34+ cells by stem cell factor Sall4B
... 7.01 ± 1.18 % in day 0 primate CD34 + cells, day 9 ex- panded primate CD34 + cells, day 9 GFP-control lentiviral transduced CD34 + cells, and day 9 GFP-Sall4B lentiviral transduced CD34 + cells, respectively (Fig. 6b). ...
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