linked immunospot assay
Selected RD1 Peptides for Active Tuberculosis Diagnosis: Comparison of a Gamma Interferon Whole-Blood Enzyme-Linked Immunosorbent Assay and an Enzyme-Linked Immunospot Assay
... enzyme-linked immunospot assay (ELISPOT), using selected early secreted antigenic target 6 (ESAT-6) peptides, that appears specific for active tuberculosis ...whole-blood assay based on region ...
6
Comparison of an ESAT-6/CFP-10 Peptide-Based Enzyme-Linked Immunospot Assay to a Tuberculin Skin Test for Screening of a Population at Moderate Risk of Contracting Tuberculosis
... Screening for latent tuberculosis infection (LTBI) with the Mantoux tuberculin skin test (TST) has many limitations including false-positive results due to Mycobacterium bovis bacillus Calmette-Gue ´rin (BCG) vacci- ...
6
Enzyme-Linked Immunospot Assay Detection of Mumps-Specific Antibody-Secreting B Cells as an Alternative Method of Laboratory Diagnosis
... (ELISPOT) assay could be used as a more reliable ...ELISPOT assay following MMR vaccination of 16 previously vaccinated or naturally exposed adult ...
8
Evaluation of the Interlaboratory Concordance in Quantification of Human Immunodeficiency Virus-Specific T Cells with a Gamma Interferon Enzyme-Linked Immunospot Assay
... ELISPOT assay is highly sensitive and reproducible (48), and a multicenter comparative study of healthy donors with influenza virus and HIV peptides found that the four laboratories involved obtained consistent ...
14
Varicella-Zoster Virus-Specific Enzyme-Linked Immunospot Assay Responses and Zoster-Associated Pain in Herpes Zoster Subjects
... Table 2 shows that the GMC responses in the control group were stable from day 0 to week 6 but were higher at month 6. The assays of blood samples from day 0, week 2, and week 6 were conducted within the same ...
5
Rotavirus-specific intestinal immune response in mice assessed by enzyme-linked immunospot assay and intestinal fragment culture.
... Oral inoculation of mice with WC3 induced low frequencies of virus-specific IgA-secreting cells by ELISPOT assay; this find- ing correlated with the small quantities of IgA secreted by G[r] ...
7
Ganglioside GM1 enzyme linked immunospot assay for simple identification of heat labile enterotoxin producing Escherichia coli
... coli strains, results obtained by this procedure GM1ELISPOT correlated well with those of the GM1 enzyme-linked immunosorbent assay GM1-ELISA; in no instance were any false-positive reac[r] ...
5
Diagnosis of Active Tuberculosis in China Using an In-House Gamma Interferon Enzyme-Linked Immunospot Assay
... ELISPOT assay. For development of an ELISPOT assay to detect specific IFN- ␥ responses against ...ELISPOT assay was 10 g/ml, as optimized by ...
6
Memory T-Cell Response to Rotavirus Detected with a Gamma Interferon Enzyme-Linked Immunospot Assay
... The IFN- ␥ ELISPOT assay we developed has added advan- tages over another IFN- ␥ ELISPOT assay recently published by Rojas et al. (31). The first advantage is in the choice of antigen. While we use pools of ...
11
Enzyme-Linked Immunospot Assay for Detection of Human Respiratory Syncytial Virus F Protein-Specific Gamma Interferon-Producing T Cells
... enzyme-linked immunospot (ELISPOT) ...maximize assay sensitivity and precision. The optimized assay uses 300,000 cells/well, 2 g/ml of an RSV F peptide pool (RSV Fpp), and incubation for 22 ⴞ ...
8
ESAT 6/CFP 10 Fusion Protein and Peptides for Optimal Diagnosis of Mycobacterium tuberculosis Infection by Ex Vivo Enzyme Linked Immunospot Assay in The Gambia
... Participants. TB index cases over 15 years of age were recruited from the major government health center in greater Banjul and the outpatient clinic at the Medical Research Council Laboratories in The Gambia. The cases ...
5
Detection of Human Papillomavirus Type 16-Specific T Lymphocytes by a Recombinant Vaccinia Virus-Based Enzyme-Linked Immunospot Assay
... Serial dilution of HPV-specific CD8 ⴙ T-cell clone cells. One limitation of using T-cell clone cells as a source of HPV- specific T cells is the difficulty in obtaining accurate cell counts. Because these T cells are not ...
7
Use of Enzyme Linked Immunospot Assay with Mycobacterium tuberculosis Specific Peptides for Diagnosis of Recent Infection with M tuberculosis after Accidental Laboratory Exposure
... From a venous blood sample peripheral blood mononuclear cells (PBMC) were isolated and an overnight gamma inter- feron (IFN- ␥ ) enzyme-linked immunospot (ELISPOT) assay was performed as previously ...
5
Evaluation of the Characteristics of the Enzyme-Linked Immunospot Assay for Diagnosis of Active Tuberculosis in China
... In patients with an acid-fast bacillus smear or in whom pathology with granuloma or caseous necrosis was detected, the T-SPOT.TB test had high positive rates (91.67% and 87.18%, respecti[r] ...
6
Comparison of Enzyme-Linked Immunospot Assay and Tuberculin Skin Test in Healthy Children Exposed to Mycobacterium tuberculosis
... T-cell assay using M tuberculosis-specific antigens is not as sensitive for the diagnosis of M tuberculosis from recent exposure as the TST in the Gambia and offers no added benefit TABLE 2 Univariable and ...
9
A phase II trial of autologous dendritic cell vaccination and radiochemotherapy following fluorescence guided surgery in newly diagnosed glioblastoma patients
... Abbreviations 5-ALA: 5-aminolevulinic; CR: complete radiological resection; DC: dendritic cell; ELISA: enzyme-linked immunosorbent assay; ELISPOT: enzyme-linked immunospot assay; FGS: fl[r] ...
12
Increase in circulating Th17 cells during anti TNF therapy is associated with ultrasonographic improvement of synovitis in rheumatoid arthritis
... In collagen-induced arthritis (CIA), a mouse model of RA, amelioration of arthritis during anti-TNF treatment has intriguingly been shown to increase the numbers of Th17 cells in draining lymph nodes, while reducing the ...
12
Potential Role of Immunodiagnosis for Pulmonary Tuberculosis Using Induced Sputum Cells
... enzyme-linked immunospot assay using induced sputum cells (IS ELISPOT) for a rapid diagnosis of pulmonary tuberculosis ...ELISPOT assay was performed using cells from induced ...
8
Comparison of Two Gamma Interferon Release Assays and Tuberculin Skin Testing for Tuberculosis Screening in a Cohort of Patients with Rheumatic Diseases Starting Anti-Tumor Necrosis Factor Therapy
... immunosorbent assay (ELISA) (QuantiFERON-TB Gold [QFT-G] and QuantiFERON-TB Gold In Tube [QFT-GIT] assays; Cellestis Limited, Carnegie, Victoria, Australia) or by enzyme-linked immunospot ...
7
Human Immunodeficiency Virus-Specific CD8+ T Cells in Human Breast Milk
... Breast-feeding infants of human immunodeficiency virus (HIV)-infected women ingest large amounts of HIV, but most escape infection. While the factors affecting transmission risk are poorly understood, HIV- specific ...
9