living-cells
Spatial and structural metrics for living cells inspired by statistical mechanics
... Experimental observations in cell biology have advanced to a stage where theory could play a larger role, much as it has done in the physical sciences. Possibly the lack of a common framework within which ...
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Model based design of RNA hybridization networks implemented in living cells
... Synthetic gene circuits allow the behavior of living cells to be reprogrammed, and non-coding small RNAs (sRNAs) are increasingly being used as pro- grammable regulators of gene expression. However, sRNAs ...
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Compartmental Genomics in Living Cells Revealed by Single-Cell Nanobiopsy
... nanobiopsy protocols. qPCR targeting GFP RNA. Positive control (red curve) consists of RNA extracted for GFP HeLa cells spiked in the cDNA synthesis mix. Nanobiopsy (blue curve) represents the average of 4 ...
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A fluorescent toolkit for spatiotemporal tracking of apoptotic cells in living Drosophila tissues
... neighboring living cells ...of living neighboring cells ...apoptotic cells in the leg disc, which has been initially visualized using the FRET sensor of caspase activity (Monier et ...
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Systems chemistry: all in a spin
... Emergence is a subtle concept that is employed in a diverse range of scientific disciplines to describe an enormous variety of fascinating phenomena. An emergent property is one that arises in a system comprised of many ...
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Real-time Characterization of Antibody Binding to Receptors on Living Immune Cells
... on living B-cells with Rituximab ...ated cells (650 nM) ...on living cells (54, ...on living cells do not differ as much from measurements on non-living material as ...
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Subcellular Localization of Thiol Capped CdTe Quantum Dots in Living Cells
... Abstract Internalization and dynamic subcellular distri- bution of thiol-capped CdTe quantum dots (QDs) in living cells were studied by means of laser scanning confocal microscopy. These unfunctionalized ...
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APOBEC3G Impairs the Multimerization of the HIV-1 Vif Protein in Living Cells
... in living cells through its PPLP ...transfected cells (80 to 90%) but were also present in the nucleus (10 to 20%), in agreement with previous observations ...
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Examination of actin and microtubule dependent APC localisations in living mammalian cells
... The APC protein plays a pivotal role in WNT signal transduction, has been suggested to have important functions in cell migration and mitosis, and APC mutation is a crucial early event in the development of most ...
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Mapping the ultrafast flow of harvested solar energy in living photosynthetic cells
... in living cells and are an essential demonstration that the isolation processes employed for decades do not significantly perturb the energy transfer ...
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Localization of CD4 and CCR5 in Living Cells
... 293T cells that expressed both CD4-YFP and CCR5-CFP were examined for localization of fluorescent proteins in the cell membrane ...in living cells ...live cells that were cotransfected with ...
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Quantum process in living cells
... of living cells whereby many degrees of freedom are reduced to a col- lective mode enabling long range coordination and or- der—a property clearly analogous to the coordination of individual processes in ...
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Herpesvirus Tegument Protein pUL37 Interacts with Dystonin/BPAG1 To Promote Capsid Transport on Microtubules during Egress
... HFFF2 cells. At 6 h, 12 h, 24 h, and 48 h postinfection, cells were scraped into the medium and ...shControl cells, although by 24 h after infection, the amount of virus produced under all 3 ...
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Characterization of Recombinant Human Cytomegaloviruses Encoding IE1 Mutants L174P and 1-382 Reveals that Viral Targeting of PML Bodies Perturbs both Intrinsic and Innate Immune Responses
... the cells (dark gray bars), HFFs were infected with different dilutions of AD169, AD169/IE1-L174P, and the IE1 deletion virus AD169 ⌬ ...infected cells and subjected to HCMV gB-specific quantitative ...
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Visualization of Intracellular Transport of Vesicular Stomatitis Virus Nucleocapsids in Living Cells
... the cells infected with the eGFP-tagged virus revealed that, following synthesis, the nucleocapsids are transported toward the cell periphery via a microtubule (MT)-mediated process, and the nucleocapsids were ...
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Novel Quantitative Approach for Predicting mRNA/Protein Counts in Living Cells
... Our results will show various figures related to our solutions. We first plot the variability of the number of protein in cells over time for a sample of 50. Next, we plot the solutions of (4) over time and ...
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A Fluorescence Ratiometric Probe for Detection of Cyanide in Water Sample and Living Cells
... NMR spectra were recorded on a Bruker Avance 400 spectrometer operating at 400 MHz and 100 MHz re- spectively. Elemental (C, H, N) analysis were carried out using Flash EA 1112 analyzer. Electronic absorption spectra ...
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Mobility of Human Immunodeficiency Virus Type 1 Pr55Gag in Living Cells
... GFP), cells were cotransfected with free monomeric red fluorescent protein (mRFP) along with the photoactivatable protein in order to locate transfected ...
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Stem cells living with a Notch
... stromal cells and inactivation of Notch1 in HSCs revealed no effect on HSC maintenance, therefore excluding an essential contribution of Jag1-mediated Notch1 signaling for adult HSC self-renewal (Mancini et ...
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High Resolution pH Imaging of Living Bacterial Cells To Detect Local pH Differences
... Schematic diagram of the bacterial flagellar basal body with a type III export apparatus attached. The export apparatus consists of a PMF-driven transmem- brane export gate made of FlhA, FlhB, FliO, FliP, FliQ, and FliR ...
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