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long-term cell cultures

Unregulated proliferation of primitive neoplastic progenitor cells in long term polycythemia vera marrow cultures

Unregulated proliferation of primitive neoplastic progenitor cells in long term polycythemia vera marrow cultures

... inoculum long-term marrow ...the long-term marrow culture system supports the continuous proliferation of primitive neoplastic progenitor cells from PV patients for many ...normal ...

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Paracrine regulation of fat cell formation in bone marrow cultures via adiponectin and prostaglandins

Paracrine regulation of fat cell formation in bone marrow cultures via adiponectin and prostaglandins

... have long been suspected to have an influence on hematopoiesis ...blood cell formation in culture, and fully differentiated fat cells produce less colony stimulating factor–1 than their precursors do ...

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Evolution of malignant plasmacytoma cell lines from K14E7 Fancd2 mouse long-term bone marrow cultures

Evolution of malignant plasmacytoma cell lines from K14E7 Fancd2 mouse long-term bone marrow cultures

... long-term marrow culture were plated in triplicate in semi- solid medium consisting of methylcellulose in IMDM, using a GelCount colony counter (Oxford Optronix, Oxford, UK) and maintained in a high ...

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On the effect of long-term electrical stimulation on three-dimensional cell cultures: Hen embryo brain spheroids

On the effect of long-term electrical stimulation on three-dimensional cell cultures: Hen embryo brain spheroids

... depending upon the standard deviation and the offset of noise. A set of data was monitored and raw signal, fi ltered signal and spikes were chosen in order to perform fast and reliable recordings and analysis with the MC ...

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Cytotoxicity patterns of arsenic trioxide exposure on HaCaT keratinocytes

Cytotoxicity patterns of arsenic trioxide exposure on HaCaT keratinocytes

... Long-term cultures were established by culturing HaCaT cells on collagen IV. MTT cytotoxicity assay was used to evaluate chronic toxicity of arsenic trioxide on keratinocytes. An LD 10 value was ...

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Expression of human bone related proteins in the hematopoietic microenvironment

Expression of human bone related proteins in the hematopoietic microenvironment

... human long-term bone marrow cell cultures; the bone protein-producing cells (BPPC) are observed under serum-free, short-term culture conditions, respond to bone- related and not ...

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Atypical multinucleated cells form in long term marrow cultures from patients with Paget's disease

Atypical multinucleated cells form in long term marrow cultures from patients with Paget's disease

... in long-term cultures of marrow mononuclear cells derived from patients with Paget's disease, and compared these with similar cells formed in long-term marrow cultures from ...

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Recombinant IL 7/HGFβ efficiently induces transplantable murine hematopoietic stem cells

Recombinant IL 7/HGFβ efficiently induces transplantable murine hematopoietic stem cells

... in cultures of mouse ...multiple cell sources, including unseparated BM cells, purified HSCs, CD45 – BM cells, and embryonic stem ...the cell cycle and exhibited reduced oxidative stress, decreased ...

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A simple and efficient method for the long-term preservation of plant cell suspension cultures

A simple and efficient method for the long-term preservation of plant cell suspension cultures

... permitting cell growth, and without the addition of growth inhibitors, did not generate long- lasting physiological and metabolic ...standard cell culture ...full cell respiration and growth ...

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Characterization of long-term cultures of hepatitis C virus.

Characterization of long-term cultures of hepatitis C virus.

... FIG. 4. NS5 region (nt 8787 to 8912; aa 2816 to 2857) of HCV in inoculum and cell cultures. The nested primer sets were nt 8737 to 8752, 59-AGAGGGTCTA CTACCT-39, and nt 8947 to 8932, 59-CCGTAGATCTCGCAGT-39, ...

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ANTICANCER POTENTIAL OF SOLANUM JASMINOIDES

ANTICANCER POTENTIAL OF SOLANUM JASMINOIDES

... Short Term Cytotoxicity Studies: The short term toxicity studies were carried out against DLA and EAC cells using standard procedures 7 ...The long term toxicity study was carried out by the ...

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JBC-2005-Ribeiro-10202-9.pdf

JBC-2005-Ribeiro-10202-9.pdf

... (4). Cultures were maintained at an air-liquid surface interface, and polarized primary cultures were studied at 6 –11 days (short term monolayers) or 30 – 40 days later (long term well ...

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Inhibition of osteoclast like cell formation by bisphosphonates in long term cultures of human bone marrow

Inhibition of osteoclast like cell formation by bisphosphonates in long term cultures of human bone marrow

... The effect of bisphosphonates on the formation of osteoclasts from their mononuclear hematopoietic precursors was investigated using human long-term marrow cultures in which multinucleat[r] ...

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Stiffness of Cell Micro-Environment Guides Long Term Cell Growth in Cell Seeded Collagen Microspheres

Stiffness of Cell Micro-Environment Guides Long Term Cell Growth in Cell Seeded Collagen Microspheres

... a cell source for tissue engineering of skeletal tissue in cell-based ...in cell differentiation and can be implemented as a guide to study cellular response, matrix production and tissue ...and ...

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Short-term physiological hypoxia potentiates the therapeutic function of mesenchymal stem cells

Short-term physiological hypoxia potentiates the therapeutic function of mesenchymal stem cells

... One such parameter is expansion of MSCs under hyp- oxia. A large number of studies demonstrated that MSCs cultured in hypoxia exhibit higher rates of proliferation and better retain their stem cell properties [25, ...

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Ultrastructural Study of Long-Term Measles Infection in Cultures of Hamster Dorsal-Root Ganglion

Ultrastructural Study of Long-Term Measles Infection in Cultures of Hamster Dorsal-Root Ganglion

... The longer life-span of measles-infected nervous tissue explants permitted expanded chronological study, which revealed that cytoplasmic viral material appeared before intranuclear inclu[r] ...

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My contribution to the debate ‘Are we all ethnomusicologists now?’

My contribution to the debate ‘Are we all ethnomusicologists now?’

... My aim is not to eliminate ethnography, or to expunge it from our anthropological consciousness. Nor is it to underrate its significance, and the complex demands it places on those who practice it. Rather, I am concerned ...

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Osteoclast like cells form in long term human bone marrow but not in peripheral blood cultures

Osteoclast like cells form in long term human bone marrow but not in peripheral blood cultures

... multinucleated cells fulfill the functional criteria for osteoclasts. Although cultured peripheral blood monocytes can be induced to form multinucleated cells with 1,25-dihydroxyvitamin D3, these cells did not show ...

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"Clone": the history of a euphonious scientific term

"Clone": the history of a euphonious scientific term

... The application of the term clone by Sanford and colleagues to mammalian cell cultures originating from a single tissue cell-a technique first successfully performed in 1948-further exte[r] ...

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Do clinical microbiology laboratories report complete bacteriology in urine from patients with long term urinary catheters?

Do clinical microbiology laboratories report complete bacteriology in urine from patients with long term urinary catheters?

... During a prospective study of the complications associated with long-term indwelling urethral catheters in two Baltimore nursing homes, we noted that reports on urine cultures performed [r] ...

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