LRP6/5
Hypomorphic expression of Dkk1 in the doubleridge mouse: dose dependence and compensatory interactions with Lrp6
... (Invitrogen). RNA (<1 µg) was treated with Dnase I (Invitrogen) and first-strand cDNA was synthesized with Superscript II reverse transcriptase (Invitrogen) using random hexamer primers. The 3′ untranslated region ...
10
Canonical WNT signaling components in vascular development and barrier formation
... protein 5 (LRP5) and LRP6 had redundant functions in brain vascular development and barrier maintenance; however, loss of LRP5 alone dramatically altered development of the retinal ...
23
Kremen is required for neural crest induction in Xenopus and promotes LRP6 mediated Wnt signaling
... of LRP6 MO in ...of 5 ng CoMO. (b,c) Co-injection of 5 ng LRP6 MO and either 1 ng control (PPL) (b) or 400-600 pg human LRP6 mRNA ...or LRP6 MO show the displayed phenotypes at ...
9
Gigantol inhibits Wnt/β catenin signaling and exhibits anticancer activity in breast cancer cells
... Cell lysates were prepared with buffer containing 20 mM Tris·HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyro- phosphate, 1 mM β-glycerol phosphate, 1 mM sodium orthovanadate, 2 μg/mL ...
8
PTK7 modulates Wnt signaling activity via LRP6
... and LRP6 protein depletion gives identical ...the LRP6-MO (25 ...endogenous LRP6 protein. Whereas LRP6 protein levels were reduced, the PTK7-MO did not reduce lrp6 mRNA levels (not ...
12
Developmental vascular regression is regulated by a Wnt/β catenin, MYC and CDKN1A pathway that controls cell proliferation and cell death
... (P) 5 wild-type ...7.0, Lrp6 was expressed at a level higher than Lrp5 (FPKM of ...that LRP6 might function alongside LRP5 to mediate Wnt/ β -catenin ...
12
The Wnt co receptors Lrp5 and Lrp6 are essential for gastrulation in mice
... the Lrp6 gene. Primers used to amplify the wild-type allele, Lrp6-U1 and Lrp6- D1 are indicated by ...allele, Lrp6-U1 and en2 are indicated by ...
13
Wnt11/β catenin signaling in both oocytes and early embryos acts through LRP6 mediated regulation of axin
... In total, five embryos or oocytes were homogenized in 200 l of ice-cold PBS plus protease inhibitors (PIC P8340 Sigma 1:100, PMSF 10 g/ml) and spun for 5 minutes at 700 g, 4°C. Supernatants were precipitated ...
11
Lrp6 is required for convergent extension during Xenopus gastrulation
... of Lrp6 morpholino (LRP6MO, 40 ng) or mRNA (1 ng) cause defects in neural and mesodermal convergent ...altered Lrp6 levels are not fully elongated (less than 70% of the length of controls) and have split or ...
12
Reduction of miR-29c enhances pancreatic cancer cell migration and stem cell–like phenotype
... (FRAT2-3’UTR), LRP6 (LRP6-3’UTR), FZD4 (FZD4- 3’UTR) and FZD5 (FZD5-3’UTR) genes, generated by PCR amplification from hTERT-HPNE, were cloned into the SacI/XmaI sites of pGL3 luciferase reporter plasmid ...
12
VSTM2A suppresses colorectal cancer and antagonizes Wnt signaling receptor LRP6
... Figure 2. VSTM2A inhibits CRC cell growth in vitro and in vivo. (A) Effects of VSTM2A on CRC cell proliferation were evaluated by MTS assay. (B) Effect of VSTM2A on inhibition of colony formation of CRC cells. CRC cells ...
15
Circ_0000527 promotes the progression of retinoblastoma by regulating miR-646/LRP6 axis
... CircRNAs, derived from reverse splicing or exon skip- ping of pre-mRNAs [5], is stable due to its special ring structure and resistant to exonuclease [22]. The role of circRNA in tumorigenesis and cancer ...
12
Arrow (LRP6) and Frizzled2 cooperate to degrade Wingless in Drosophila imaginal discs
... Dextran labelling of imaginal discs shows that Wingless is still internalized by arrow mutant cells (see Fig. S3 in the supplementary material), suggesting that Frizzled and Frizzled2 may not require Arrow for ...
11
Role of Hydrophobic Patch in LRP6: A Promising Drug Target for Alzheimer's disease
... including LRP6 and DKK1 (PDB ID - 3S8V) were identified by the ...of LRP6 and ...(4S,5Z,6S)- 5-[2-[(E)-3-(4-hydroxy-3-methoxy-phenyl) prop-2-enoyl]oxyethylidene]-4-(2-methoxy-2- oxo-et, ZINC70665802 ...
12
Original Article MicroRNA-128 suppresses colorectal cancer cell proliferation through targeting LRP6
... and 5 CRC cell lines was detected by quantitative real-time PCR ...that LRP6 was a direct and functional target of ...of LRP6 inhibited CRC cell ...
9
Cardiomyocyte-Restricted Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) Deletion Leads to Lethal Dilated Cardiomyopathy Partly Through Drp1 Signaling
... of LRP6 in coronary heart disease owing to high blood lipid has been reported [7, ...in LRP6 weakens the wnt3a signaling pathway and impairs endothelial cell functions, which contributes to normolipidemic ...
17
Induction of LEF1 by MYC activates the WNT pathway and maintains cell proliferation
... To knockdown target genes, 50 nM of siRNA was reverse transfected to 2.5 × 10 5 cells in 6-well plates with Lipofecta- mine RNAiMAX reagent. Total protein lysates were ex- tracted with NP40 Lysis buffer (50 mM ...
16
The LRP6 rs2302685 polymorphism is associated with increased risk of myocardial infarction
... that LRP6, as a component of LDL cholesterol trafficking complex, was involved in direct LDL uptake [37]; and the elevated LDL-C levels in LRP6 R611C mutation carriers was likely due to the reduced LDL ...
6
Maintenance of stemness is associated with the interation of LRP6 and heparin-binding protein CCN2 autocrined by hepatocellular carcinoma
... and LRP6 enhance malignant phenotypes of HCC To evaluate the roles of CCN2 and LRP6 in HCC, first, we silenced the endogenous CCN2 expression in MHCC-97H ...of LRP6 in HCC, MHCC-97H cells were ...
15
Extracellular LDLR repeats modulate Wnt signaling activity by promoting LRP6 receptor endocytosis mediated by the Itch E3 ubiquitin ligase
... pCMV-flag- LRP6-D1315N plasmids and inserted into the NSBI lentiviral vector backbone [30] using standard cloning ...His-S1317P LRP6 was generated by cloning in frame a His (6x) sequence CACCACCACCACCACCAC ...
15