LS174T Cells
Teng-Long-Bu-Zhong-Tang induces p21-dependent cell senescence in colorectal carcinoma LS174T cells via histone acetylation
... untreated LS174T cells were collected and assessed for HDAC activity with a specific kit, according to the manufacturer’s ...the cells were lysed at 4°C for 5 minutes and centrifuged at 12,000 rpm ...
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Original Article CSN5 silencing inhibits invasion and arrests cell cycle progression in human colorectal cancer SW480 and LS174T cells in vitro
... SW480 cells and LS174T cells were either subjected to mock transfection without shRNA (lane 1) or transfected with negative-control (NC) shRNA (lane 2) or the indicated CSN5 shRNas (lanes 3 and ...in ...
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<p>Upregulated SET Promotes Cell Survival Through Activating Akt/NF-κB Signal in Colorectal Carcinoma</p>
... CRC cells leaded to a signi fi cantly increased of the activity of ...CRC cells (Figure ...treated Ls174T cells with siRNA-PP2A and SC79, a highly Akt ...
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Bilirubin attenuates ER Stress Mediated Inflammation, escalates Apoptosis and reduces Proliferation in the LS174T Colonic Epithelial Cell Line
... potential cancer treatments [42, 43] including the use of tetrapyrrolic bile pigments in human cancer cells [44]. Apart from being used as a novel biomarker for nasopharyngeal carcinoma [45], UCB has the potential ...
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Interplay between Endoplasmic Reticular Stress and Survivin in Colonic Epithelial Cells
... on LS174T cells treated with TUN and YM155 alone and noticed that YM155 could reduce the gene expression and protein expression of UPR genes in parallel to reducing Survivin expression along with the ...
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The ratio of STAT1 to STAT3 expression is a determinant of colorectal cancer growth
... and LS174T cells (Figure 3c and ...and LS174T (high STAT1+low STAT3) resulted in growth reduction, as assessed by tumor growth curves (Figure 4a) and tumor weight at end point analysis (Figure ...and ...
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The histone deacetylase inhibitor trichostatin A induces cell cycle arrest and rapid upregulation of gadd45β in LS174T human colon cancer cells
... line LS174T. Addition of TSA to LS174T cells induced inhibition of cell prolifera- tion by arresting the cell ...of LS174T cells induced rapid upregulation of gadd45β mRNA expression ...
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Engineering of near IR fluorescent albumin nanoparticles for in vivo detection of colon cancer
... For optimization of the tumor detection process, PNA was covalently conjugated to the nanoparticles as described in the experimental part. PNA binds to the terminal sugar β-D-galactosyl-(1–3)-N-acetyl-D-galact- osamine ...
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MicroRNA-552 enhances metastatic capacity of colorectal cancer cells by targeting a disintegrin and metalloprotease 28
... and cells by a miRNA profiling ...and LS174T CRC cells by transducing miR-552 inhibitor (antagomiR-552) with a lentiviral vector exhibited an ability to reduce cell proliferation, migration and ...
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TLE3 represses colorectal cancer proliferation by inhibiting MAPK and AKT signaling pathways
... and Ls174t cells with TLE3 overex- pressing, whereas increased in HCT15 and SW620 cells with TLE3 knocking down in comparison with control cells ...and Ls174t cells with TLE3 ...
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Irinotecan treatment and senescence failure promote the emergence of more transformed and invasive cells that depend on anti-apoptotic Mcl-1
... cancer cells adapt to this genotoxic drug ...of LS174T cells can tolerate this treatment, escape senescence and emerge as a dividing ...persistent cells regained drug sensitivity. In addition, ...
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Bitter melon protects against ER stress in LS174T colonic epithelial cells
... treated LS174T cells by firstly washing the cells with HBSS followed by homogenization in 2 mL of RIPA buffer/ 10% of Protease Inhibitor (Sigma-Aldrich, AUS) and then cleared by centrifugation at ...
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Expression profiling and intracellular localization studies of the novel Proline-, Histidine-, and Glycine-rich protein 1 suggest an essential role in gastro-intestinal epithelium and a potential clinical application in colorectal cancer diagnostics
... The protein fraction was extracted from 5 to 10 million LS174T cells by the Allprep DNA/RNA/protein Mini kit according to the manufacturer’s protocol (Qiagen) and dissolved in 5% SDS. The SDS was ...
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CEA-targeted nanoparticles allow specific in vivo fluorescent imaging of colorectal cancer models.
... All procedures were licensed by UK Home Office and were carried out in accordance with local ethical review. 4-6 week old BALB/c nu/nu female mice (Charles River, UK) were injected subcutaneously with 1.5x10 6 ...
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Anti-Tumor Efficacy of a Novel Antisense Anti-MDM2 Mixed-Backbone Oligonucleotide in Human Colon Cancer Models: p53-Dependent and p53-Independent Mechanisms
... In LS174T cells that contain wild-type p53, the cancer chemotherapeutic agents HCPT, adriamycin, and 5-FU induced p53 levels, but this was limited owing to MDM2 ...
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Engineering of near infrared fluorescent proteinoid-poly(L-lactic acid) particles for in vivo colon cancer detection
... (Switzerland); LS174t, SW480 and HT29 cell lines from American Type Culture Collection (ATCC); donkey anti-rabbit IgG from Jackson Immuno- Research Laboratories (West Grove, PA, USA); water was purified by passing ...
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Clinical applications of dendritic cells–cytokine-induced killer cells mediated immunotherapy for pancreatic cancer: an up-to-date meta-analysis
... Literature screening and data extraction were carried out by two independent reviewers (YZ and XZ), and disagreements were eliminated upon discussing with a third researcher (AZ). Extracted information included first ...
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Embryonic stem cells differentiate into insulin producing cells without selection of nestin expressing cells
... Cells were suspended in lysis buffer (4 M guanidinium thiocyanate, 25 mM sodium citrate, pH 7; 0.5% sarcosyl, 0.1 M β -mercaptoethanol). Total RNA was isolated by the single step extraction method according to ...
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124I HuCC49deltaCH2 for TAG 72 antigen directed positron emission tomography (PET) imaging of LS174T colon adenocarcinoma tumor implants in xenograft mice: preliminary results
... described metabolic effects resulting from only a short- duration fast should have been minimized. Furthermore, these same authors reported that Ketamine/Xylazine anesthesia did not significantly alter 18 F-FDG activity ...
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Unravelling the specificity and mechanism of sialic acid recognition by the gut symbiont Ruminococcus gnavus
... determination, cells were collected by centrifugation, resuspended in phosphate buffered saline (PBS, 150 mM sodium chloride, 10 mM sodium phosphate, pH ...
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