Luciferase Reporter
Cloning Of Anopheles Gambiae CYP6M2 Gene Promoter And Construction Of Its Luciferase Reporter System
... its luciferase reporter system in Anopheles ...the luciferase expression vector pGL3-Basic ...dual luciferase reporter system were successfully established, this will be essential in ...
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Luciferase Reporter Mycobacteriophages for Detection, Identification, and Antibiotic Susceptibility Testing ofMycobacterium tuberculosis in Mexico
... of luciferase reporter mycobacteriophages (LRPs) for detection, identification, and antibiotic susceptibility testing of Mycobacterium tuberculosis was prospectively evaluated in a clinical microbiology ...
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Characterization of the Early Steps of Hepatitis C Virus Infection by Using Luciferase Reporter Viruses
... JFH1-derived luciferase reporter ...the reporter gene used in this study are shown ...of reporter constructs, viral proteins are expressed via an internal EMCV IRES (gray ...The ...
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Original Article HOTAIR contributes to chemoresistance by activating NF-κB signaling in small-cell lung cancer
... Dual luciferase reporter assays and a western blot an- alysis showed that both the NF-κB induced luciferase activ- ity and the expression of BCL- XL were decreased after treat- ment with celastrol in ...
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β-Adrenoreceptors Reactivate Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication via PKA-Dependent Control of Viral RTA
... by luciferase reporter assays in which pRpluc (firefly luciferase coding sequence controlled by ⬃ 3 kb of KSHV genomic DNA upstream of ORF50) was electroporated into the Ramos or DG75 B cell lines, ...
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Human Immunodeficiency Virus Type 1 Vpr Induces the Degradation of the UNG and SMUG Uracil-DNA Glycosylases
... A Wild-type, ⌬vif, ⌬vpr, and ⌬vif ⌬vpr luciferase reporter viruses were produced in 293T cells cotransfected with the indicated amounts of human APOBEC3G expression vector.. Luciferase a[r] ...
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MiR-10a-5p targets TFAP2C to promote gemcitabine resistance in pancreatic ductal adenocarcinoma
... a Luciferase reporter constructs containing downstream wild-type (WT) and mutated (MUT) binding sequences in TFAP2C; b the luciferase activity of the WT and MUT groups suggests that TFAP[r] ...
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LINC00667/miR-449b-5p/YY1 axis promotes cell proliferation and migration in colorectal cancer
... the decreased levels were determined separately by qRT- PCR and western blot (Fig. 2b, c). In luciferase reporter assay, only YY1 down-regulation restrained the activ- ity of LINC00667 promoter in both CRC ...
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Transcription factor CCAAT/enhancer binding protein alpha up-regulates microRNA let-7a-1 in lung cancer cells by direct binding
... Methods: The DNA sequence of the 2.1 kb let-7a-1 promoter was analyzed with MatInspector 4.1 (http://www.geno- matix.de). Human lung cancer cell lines A549 and H1299, and human cervical cancer cell line Hela were used ...
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Original Article miR-135a acts as a tumor suppressor by targeting ASPH in endometrial cancer
... We employed TargetScan (www.targetscan. org/) to predict the potential targets of miR- 135a. Cells were plated in 96-well plates and co-transfected with miR-135a mimic or NC and wild-type (wt) or mutant (mut) ASPH 3’-UTR ...
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Hypoxia-inducible MiR-182 promotes angiogenesis by targeting RASA1 in hepatocellular carcinoma
... dual luciferase reporter results in SK-HEP-1 cells showed that the transfection of miR-182 mimics exerted repressive effects of luciferase activity of RASA1 3’UTR plasmid while the inhibition of ...
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Extrachromosomal HPV-16 LCR transcriptional activation by HDACi opposed by cellular differentiation and DNA integration
... Histone deacetylase inhibitors (HDACi) have been shown to render HPV- carrying cells susceptible to intrinsic and extrinsic apoptotic signals. As such, these epigenetic drugs have entered clinical trials in the effort to ...
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Differential Functions of Interferon-Upregulated Sp100 Isoforms: Herpes Simplex Virus Type 1 Promoter-Based Immediate-Early Gene Suppression and PML Protection from ICP0-Mediated Degradation
... on luciferase expression from the ICP0 ...a luciferase reporter plasmid under the control of the ICP0 promoter or the minimum CMV promoter–  -galactosidase reporter; equal amounts of ...
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Pegylated leptin antagonist with strong orexigenic activity in mice is not effective in chickens
... and luciferase reporter genes were stimulated with human leptin and with interferon αA (IFNA2), both activating the reporter gene through the cLEPR or an endogenous IFNR, respectively (Hen et ...of ...
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Up-regulation of human cervical cancer proto-oncogene contributes to hepatitis B virus-induced malignant transformation of hepatocyte by down-regulating E-cadherin
... site-mutated reporter vector, pGL3-HCCRP -166/+30tcf1m , and transfected it into Huh-7 cells along with ...the reporter assay showed that mutating the tcf-1 site essentially abolished the effects of HBx on ...
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<p>Circ_0000517 Contributes to Hepatocellular Carcinoma Progression by Upregulating TXNDC5 via Sponging miR-1296-5p</p>
... Figure 4 MiR-1296-5p regulated HCC cell viability, cell cycle process, colony formation and apoptosis by targeting TXNDC5.(A) The complementary sequences between miR-1296-5p and TXNDC5 were presented. (B) The targeting ...
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Identification of a novel human long non-coding RNA that regulates hepatic lipid metabolism by inhibiting SREBP-1c
... promoter reporter gene was co-transfected with either lncHR1 overexpression (D) or knockdown (E) constructs in Huh7 cells, and luciferase reporter expression was measured and ...
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miR-450b-5p induced by oncogenic KRAS is required for colorectal cancer progression
... For luciferase assay, small regions containing the target sequences of mir-450b-5p in target genes 3’-UTR were generated by PCR amplification and cloned into pGL3-basic luciferase reporter plasmid ...
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Establishment and Characterization of a Madin Darby Canine Kidney Reporter Cell Line for Influenza A Virus Assays
... virus reporter cell line that contributes to more efficient viral detection in cell ...genome-like luciferase reporter RNA driven by the canine RNA polymerase I ...of luciferase activity was ...
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Long noncoding RNA HOTAIR promotes breast cancer development by targeting ZEB1 via sponging miR-601
... Dual-luciferase reporter assay results showed that miR-601 overexpression markedly suppressed the luciferase activity of ZEB1 3 ′ UTR-WT reporter, while had no effect on the lucif- erase ...
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