Lysate Antigens
Blastomyces dermatitidis: Stability studies on different yeast lysate antigens
... the antigens, produced from 1989 to 2012 and stored at 4˚C, was deter- mined by comparing antibody detection (enzy- me-linked immunosorbent assay; ELISA) in 12 serum specimens from immunized ...19 antigens ...
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Comparison of Antibody Detection with Yeast Lysate Antigens Prepared from Blastomyces dermatitidis Dog Isolates from Wisconsin and Tennessee
... yeast lysate reagents for the detection of antibodies in ...use lysate antigens prepared from four B. dermatitidis antigens isolated from dogs infected with blastomycosis from two different ...
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Blastomyces dermatitidis Antibody Responses in Serial Serum Specimens from Dogs with Blastomycosis: Comparison of Different Yeast Lysate Antigens
... yeast lysate antigens, prepared from dog and human isolates, for their ability to detect antibodies in serial serum specimens, obtained at various intervals post-treatment, from dogs with diagnosed ...
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Comparison of antibody detection with Blastomycesdermatitidis yeast lysate antigens in serum specimens from immunized rabbits and infected dogs
... phase lysate preparations and utilized these antigens in the ELISA for the detection of antibodies in serum specimens from immunized or infected animals ...yeast lysate antigens as ...
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Detection of Antibody in Dogs with Blastomycosis Using Blastomyces dermatitidis Yeast Phase Lysate Antigens
... sate antigens [ERC-2, dog Wisconsin; 85, soil Georgia, ATCC 56,920] for de- tecting antibody in 38 serum specimens [pre-treatment, 30-day, and 60-day post treatment] from dogs with diagnosed ...two antigens ...
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Comparison of Detection of Antibodies with Yeast Lysate Antigens Prepared from Two Isolates of Blastomyces dermatitidis by Two Different Methods: Sensitivity and Specificity Evaluations
... develop antigens with a high degree of sensitivity and ...yeast lysate antigens, prepared from two ...yeast lysate re- agents prepared after allowing yeast cells to lyse for 1 or 7 ...7 ...
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Detection of Antibodies in Serum Specimens from Dogs with Blastomycosis with Lysate Antigens Prepared from Four Blastomyces dermatitidis Dog Isolates: Individual Antigens vs Antigen Combinations
... individual lysate reagents, but little with respect to evaluating antigen combinations for anti- body ...yeast lysate antigens prepared from 4 ...
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Use of Viral Lysate Antigen Combined with Recombinant Protein in Western Immunoblot Assay as Confirmatory Test for Serodiagnosis of Severe Acute Respiratory Syndrome
... Western immunoblot assays have been routinely used as confirmatory tests for the last decade and remain important tools for managing viral infections such as those caused by human immunodeficiency virus, human T-cell ...
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Expression and Refolding of Truncated Recombinant Major Outer Membrane Protein Antigen (r56) of Orientia tsutsugamushi and Its Use in Enzyme-Linked Immunosorbent Assays
... whole-cell lysate of the Karp strain of ...whole-cell lysate antigens correlated well (r 5 ...control antigens (specificity 5 92%, n 5 13) using a positive cutoff value determined with eight ...
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Enhanced stimulation of anti-breast cancer T cells responses by dendritic cells loaded with poly lactic-co-glycolic acid (PLGA) nanoparticle encapsulated tumor antigens
... tumor lysate antigen obtained from three stage III breast cancer tissues encapsulated within PLGA NPs to enhance the DC maturation was ...tumor lysate antigens containing poly lactic-co-glycolic acid ...
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The mechanism of action of a single dose of methylprednisolone on acute inflammation in vivo
... VNL, volume of neutrophil lysate; TN, total number of cells in the lysate in millions, based on cell counts done on the inflammatory fluid before centrifugation and lysis; ENIC, intracel[r] ...
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The Ability of Lysate PRF Induces Proliferation of Fibroblast Cells in Endodontic Regenerative Therapy
... Platelet rich fibrin (PRF) is a blood-processed product with one centrifugation step and no additional anticoagulants and bovine serum [4]. Lysate-PRF is part of a PRF containing cytokines, structured ...
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Whole gel processing procedure for GeLC-MS/MS based proteomics
... Background: SDS-PAGE followed by in-gel digestion (IGD) is a popular workflow in mass spectrometry-based proteomics. In GeLC-MS/MS, a protein lysate of a biological sample is separated by SDS-PAGE and each gel ...
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AN OVERVIEW OF LIMULUS AMOEBOCYTE LYSATE (LAL) TEST
... Limulus Amoebocyte Lysate (LAL) test is in vitro test to detect and measure bacterial endotoxins. It is the most critical quality control test required by FDA for all parenteral drugs in their final stages of ...
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Identification of Pancreatic Cancer Associated Tumor Antigen from HSP Enriched Tumor Lysate Pulsed Human Dendritic Cells
... tumor-associated antigens are advantageous, as they lower the chance of tumor evasion of the immune response by antigen deletion or mutation and circumvent the need for identification of TAAs from indi- vidual ...
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Inhibition of Nef- and phorbol ester-induced CD4 degradation by macrolide antibiotics.
... In vitro transcription and translation. Full-length human CD4 mRNA was transcribed in vitro from pcDNA.T4 (kindly provided by Ned Landau, Aaron Diamond AIDS Research Center, New York) (27). HIV-1 Nef mRNA was transcribed ...
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Pathogen free, plasma poor platelet lysate and expansion of human mesenchymal stem cells
... Platelet lysate (PL) contains a wide series of growth factors, thanks to which platelets (PLT) are capable to mediate tissue repair at in- jured sites in physio-pathological conditions; for these reasons it has ...
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Single step, chromogenic Limulus amebocyte lysate assay for endotoxin
... With a multiplereagent chromogenic method in which the enzyme is activated by LAL in the first step and color is developed after addition of the buffer and the substrate in the second st[r] ...
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Turbidimetric method for quantifying serum inhibition of Limulus amoebocyte lysate
... When the inhibition of LPS in the presence of serum was compared by using different types of LPS, the more potent LPS types determined in saline were inhibited to a greater degree than t[r] ...
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A thymidylate synthase ternary complex-specific antibody, FTS, permits functional monitoring of fluoropyrimidines dosing
... final concentrations of 1mg/ml, 50µg/ml, and 50µg/ml, respectively. To lyse bacteria, TritonX was added to a final concentration of 1% and the mixture vortexed and freeze-thawed thrice using liquid nitrogen. The ...
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