Lysosomal degradation
Hepatitis C Virus NS5A Protein Promotes the Lysosomal Degradation of Hepatocyte Nuclear Factor 1α via Chaperone-Mediated Autophagy
... the degradation of cellular constituents in the ...bulk degradation process. In contrast, CMA is a selective degradation pathway that targets only proteins containing a pentapeptide CMA- targeting ...
14
Concurrent detection of autolysosome formation and lysosomal degradation by flow cytometry in a high-content screen for inducers of autophagy
... with lysosomal degradation as the limiting step in ...although degradation steps could be efficiently blocked by addition of Baf, our assay does not rely on addition of lysosomal inhibitors to ...
15
Inhibition of Endosomal/Lysosomal Degradation Increases the Infectivity of Human Immunodeficiency Virus
... that the discrepancies between these two studies are due to differences in the experimental approaches employed. Fackler and Peterlin initiated their infections at the same time the lysosomotropic agents were added (11). ...
7
Targeting lysosomal degradation induces p53 dependent cell death and prevents cancer in mouse models of lymphomagenesis
... impaired lysosomal protein ...induces lysosomal stress and provokes a p53-dependent cell death that does not require caspase-mediated ...lysosome-mediated degradation may be effective in cancer ...
11
SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways.
... SNX17mediated lysosomal retrieval, it appears that SNX17, unlike its family member SNX27, is not an adaptor for the retromer ...the lysosomal degradation of ...enhanced lysosomal ...
12
HSV 2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway
... including lysosomal degradation, protea- somal degradation, and/or sequestration/retargeting of tetherin to the trans-Golgi network [16, 21, 41 – ...the degradation of tetherin in ...
9
Inactivation of lysosomal proteases by oxidized low density lipoprotein is partially responsible for its poor degradation by mouse peritoneal macrophages
... macrophage lysosomal proteases has been documented and attributed to modifications in apo ...of lysosomal proteases by ox-LDL could also be responsible for this deficient ...subsequent degradation of ...
8
Inhibitors of protein synthesis also inhibit lysosomal proteolysis Studies using cystinotic fibroblasts
... inhibit lysosomal proteolysis of these ...the degradation of the extracellular disulfide-rich proteins, albumin and RNase, but lysosomal cystine accumulation derived from insulin is inhibited by ...
8
Autophagy defends pancreatic β cells from human islet amyloid polypeptide induced toxicity
... p62 acts as an autophagic receptor for polyubiq- uitinated proteins because of its ability to bind both target-associated ubiquitin and LC3 conjugated to the autophagosome membrane (8). We next sought to establish ...
13
Oxidant-induced autophagy and ferritin degradation contribute to epithelial–mesenchymal transition through lysosomal iron
... of lysosomal degradation, either by CQ or bafilomycin A1, which blocked the formation of autophagolysosomes and inhibited lysosomal degradation by raising intralysosomal pH, or by a mixture of ...
11
Pneumolysin Activates Macrophage Lysosomal Membrane Permeabilization and Executes Apoptosis by Distinct Mechanisms without Membrane Pore Formation
... in lysosomal membrane permeabiliza- ...of lysosomal proteases into the cytoplasm (20). However, lysosomal integrity could theoretically be compromised earlier to a more modest extent, allowing efflux ...
13
The relationship of physico chemical properties and structure to the differential antiplasmodial activity of the cinchona alkaloids
... at lysosomal pH (such as CQ) are more likely to pass through the mutated PfCRT channel and escape from the lysosome into the parasite cytoplasm, while those which are more hydrophobic (AQ and its active metabolite ...
14
Dysregulation of autophagy in chronic lymphocytic leukemia with the small molecule Sirtuin inhibitor Tenovin 6
... Tenovin-6 (Tnv-6) is a bioactive small molecule with anti-neoplastic activity. Inhibition of the Sirtuin class of protein deacetylases with activation of p53 function is associated with the pro-apoptotic effects of Tnv-6 ...
8
Inhibition of Amebic Lysosomal Acidification Blocks Amebic Trogocytosis and Cell Killing
... the degradation of ingested macromolecules. Lysosomal function is therefore likely to be essential to support continued trogocytosis and, consequently, host cell ...amebic lysosomal pH decreases ...
10
Quantitative Proteome Analysis of Mouse Liver Lysosomes Provides Evidence for Mannose 6-phosphate-independent Targeting Mechanisms of Acid Hydrolases in Mucolipidosis II
... ified lysosomal proteins in the trans Golgi network (TGN) and mediate their segregation from the secretory pathway ...(5). Lysosomal enzymes which escape the binding to MPRs are secreted but can be ...
13
Neuraminidase of Influenza A Virus Binds Lysosome-Associated Membrane Proteins Directly and Induces Lysosome Rupture
... Recently, lysosomal cell death has been defined as “lysosomal membrane permeabilization and the consequent leak- age of lysosome contents into ...induces lysosomal rupture, and finally leads to cell ...
12
Lysosomal function in macromolecular homeostasis and bioenergetics in Parkinson's disease
... cause lysosomal storage disease neuronal ceroid lipofus- cinosis (NCL), only tripeptidyl peptidase I and Cathepsin D are ...in lysosomal tripeptidyl peptidase I deficient patients ...
13
Astemizole Inhibits mTOR Signaling and Angiogenesis by Blocking Cholesterol Trafficking
... Astemizole (AST) and cholesterol were purchased from Santa Cruz Biotechnology (Dallas, Texas). Methyl-β-cyclodextrin, filipin, itraconazole and N-Phenylthiourea (PTU) were purchased from Sigma-Aldrich (St. Louis, MO). ...
11
Diagnostic methods for Lysosomal Storage Disease
... Lysosomal storage disorders (LSD) are a class of metabolic disturbance in which manifested by the accumulation of large molecules (complex lipids, glycoproteins, glycosaminoglycans, etc.) in lysosomes. LSDs have a ...
10
Lysosomal processing of progranulin
... Because PGRN can also be delivered to lysosomes from the extracellular space [9, 10], we wanted to deter- mine whether extracellular-derived, endocytosed PGRN can also be processed. To assess this, we treated primary Grn ...
6