M leprae
Transcription factors STAT 4, STAT 6 and CREB regulate Th1/Th2 response in leprosy patients: effect of M leprae antigens
... The prevalence of leprosy at the end of 2017 was 192,713 cases worldwide and the number of new cases reported was 210,671. India still contributes the major percentage of leprosy cases due to continued transmission in ...
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High frequency of M leprae DNA detection in asymptomatic household contacts
... The M. leprae genome sequence has made it possible to target specific sequences of the ...of M. leprae DNA [7, ...of M. leprae [12, ...for M. leprae, comparing it ...
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Development of a combined RLEP/16S rRNA (RT) qPCR assay for the detection of viable M leprae from nasal swab samples
... of M. leprae as well as the aerosol route of infection by means of nasal carriage ...biopsies, M. leprae DNA detection rates of more than 80% from SSS and nasal swab samples from clinically ...
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Immunologically reactive M leprae antigens with relevance to diagnosis and vaccine development
... the M. leprae proteins evaluated immunologically were con- ducted against the predicted proteomes of ...considered M. leprae unique, with no homolo- gues found in the other mycobacteria ...
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Rational Combination of Peptides Derived from Different Mycobacterium leprae Proteins Improves Sensitivity for Immunodiagnosis of M. leprae Infection
... five M. leprae T-cell nonresponders and seven M. leprae T-cell responders, as determined by their T-cell response (IFN-␥) to ...whole-M. leprae bacterial extracts (data not ...
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Presence of Human T-Cell Responses to the Mycobacterium leprae 45-Kilodalton Antigen Reflects Infection with or Exposure to M. leprae
... to M. leprae both in vitro and in vivo, and few bacilli are present in skin ...to M. leprae and there is uncontrolled growth of the organism in skin le- sions, often leading to disability and ...
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Diversity of Potential Short Tandem Repeats in Mycobacterium leprae and Application for Molecular Typing
... loci with strong discriminatory capacity which have been also tested for its stability and reproducibility in this study were the most likely and reliable sites for genetic diversity for M. leprae. Our ...
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Rapid Variable Number Tandem Repeat Genotyping for Mycobacterium leprae Clinical Specimens
... To improve throughput, we have developed a multiplex- PCR approach suitable for rapid amplification of 15 VNTR loci from M. leprae-containing specimens like slit skin smears and skin biopsy specimens. The ...
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Genotyping of Mycobacterium leprae on the Basis of the Polymorphism of TTC Repeats for Analysis of Leprosy Transmission
... (16). M. leprae-positive PCR products for TTC genotyping were obtained from 48 samples out of 92 previous ...M. leprae-positive samples from Gamtala and 49 samples out of 68 previous ...
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Intracellular Mycobacterium leprae Utilizes Host Glucose as a Carbon Source in Schwann Cells
... of M. tuber- culosis, so why M. leprae fails to grow in vitro remains a ...by M. leprae growing inside its human host ...intracellular M. leprae, impairment of host cell ...
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Molecular Determination of Mycobacterium leprae Viability by Use of Real Time PCR
... M. leprae viability determination in infected M ⌽ ...RT-PCR-based M. leprae viability assays to detect the effects of antileprosy drug treatment of infected M ⌽ or the effects of ...
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Recombinant polypeptide of Mycobacterium leprae as a potential tool for serological detection of leprosy
... and increases the risk of severe disabilities (van Hooij et al. 2019). Other diagnostic methods like bacilloscopy and histopathology also lack adequate sensitivity and rely on well-trained technicians as well (Cheng et ...
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ML0405 and ML2331 Are Antigens of Mycobacterium leprae with Potential for Diagnosis of Leprosy
... Multidrug therapy for treatment of early or subclinical lep- rosy could have a significant impact on both the prevention of nerve damage within infected individuals and the transmission of the M. leprae ...
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Enhancing Sensitivity of Detection of Immune Responses to Mycobacterium leprae Peptides in Whole-Blood Assays
... In order to determine whether indeed CD4 ⫹ or CD8 ⫹ T cells were responsible for IFN- ␥ production in this IL-12-en- hanced WBA with M. leprae peptide, flow cytometry-based detection of T cells was used ...
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Simultaneous Detection of Mycobacterium leprae and Its Susceptibility to Dapsone Using DNA Heteroduplex Analysis
... DNA sequencing. To identify mutations associated with DDS resistance in M. leprae strains, the folP1 SRDRs from the strains were amplified by PCR as described above. The resultant PCR products were purified ...
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Global surveillance system to monitor the development of drug resistance in <em>Mycobacterium leprae</em>
... In the mouse foot pad assay, some isolates do not multi- ply in control mouse footpads, and their drug susceptibility cannot be determined using this method. In contrast, DNA sequencing of PCR products is independent of ...
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Drug and Multidrug Resistance among Mycobacterium leprae Isolates from Brazilian Relapsed Leprosy Patients
... the M. leprae genes rpoB, folP, and gyrA, and possibly this patient, although being MDR, presented this disease form because he was diagnosed very soon after developing relapse, had a better immune host ...
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IN SILICO FINDING OF THE PUTATIVE DRUG TARGETS FROM HYPOTHETICAL SET OF PROTEINS FOR MYCOBACTERIUM LEPRAE TN
... Leprosy is a Flügge droplet infection, transmission occurs by inhalation of the bacilli contained in nasal secretion, but other possible route of transmissions of leprosy are skin erosions, blood, vertical transmission, ...
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Mycobacterium leprae Hsp65 administration reduces the lifespan of aged high antibody producer mice
... Autoimmune diseases are more frequent in aged and in female individuals [39] and thus we asked whether Hsp65 interference in autoimmunity is age and/or gender-related. Reports of Hsp65 interference in autoimmunity and ...
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Distribution of Mycobacterium leprae Strains among Cases in a Rural and Urban Population of Maharashtra, India
... The VNTR profiles obtained in this study were compared to VNTR profiles from a published M. leprae VNTR database (1, 3, 4, 7, 9, 16, 19, 21, 22) using the modified structure–nearest-neighbor method as ...
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