Magnetic Beads
Usefulness of Immuno Magnetic Beads Conjugated with Anti EpCAM Antibody for Detecting Endometrial Cancer Cells
... immuno-magnetic beads, the cells retrieved using the immuno-magnetic beads were stained with the anti-human EpCAM goat poly- clonal Ab (R & D Systems) and assessed using an imag- ing ...
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A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies
... immunomagnetic beads (IMB), respectively, which were activated by N - (3-dimethylaminopropyl)- N’ -ethylcar-bodiimide hydrochloride (EDC) and N -hydroxysuccinimide (NHS) to form ...
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A Novel Nano Magnetic Beads Dot ELISA Immunoassay and Its Application on the Detection of Giardia lamblia Coproanti-gen
... Methods: This cross-sectional study was carried out from Oct 2015 to Feb 2016 on patients admitted to Benha University Hospitals (Benha, Egypt) and outpatients of Theodor Bilharz Research Institute (TBRI) (Giza, Egypt). ...
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Specific Detection of Unamplified Mycobacterial DNA by Use of Fluorescent Semiconductor Quantum Dots and Magnetic Beads
... Here we present the development of a specific DNA detection method using fluorescent semiconductor quantum dots (QDs) and magnetic beads (MBs) for fast detection of Mycobacterium spp., dispensing with the ...
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Genomic characterization of the virus causing infectious salmon anemia in Atlantic salmon (Salmo salar L.): an orthomyxo-like virus in a teleost.
... polystyrene magnetic beads, precoated with sheep anti-mouse immunoglobulin G (Dyna- beads M-280; Dynal AS, Oslo, Norway) according to the manufacturer’s instruc- ...the beads were placed on a ...
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Resting CD4+effector memory T cells are precursors of bystander activated effectors: a surrogate model of rheumatoid arthritis synovial T cell function
... Methods Here, using magnetic beads and fluorescenceactivated cell sorting, we extensively phenotype the Tck effector cells and conclude that effector function resides within the CD4+CD45[r] ...
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Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay
... In this study, a novel simple, quantitative, and multiplex detection method was demonstrated for blood grouping through the use of QDs and magnetic beads (Figure 1). A typical QFA consists of three ...
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Electrochemical Behaviour of Doxorubicin Encapsulated in Apoferritin
... The comparison of recovery ratios of doxorubicin encapsulated/opened in apoferritin to doxorubicin encapsulated/opened in biotinylated apoferritin with magnetic beads is shown in Fig. 5A (D1 peak) and in ...
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journal.pntd.0006793.pdf
... with magnetic beads coated with rE of DENV1, 2, 3 or 4 and serotype specific IgG titers were determined against all 4 rE serotypes for A) TR and B) TNR tetravalent ...
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Clinical proteomics in oncology : a passionate dance between science and clinic
... quality magnetic beads based on hydrophobic interaction chro- matography (MB-HIC kit, Bruker Daltonics, Leipzig, Germany) were used for sample preparation prior to MALDI-TOF mass spectrometry ...µl ...
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Toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase
... alized magnetic beads were used, fractionated in 2 mL micro test ...the magnetic beads were washed for four times with ...a magnetic device for removal of the supernatant. To this 20 ...
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Isolation of High-Affinity Single-Chain Antibodies against Mycobacterium avium subsp. paratuberculosis Surface Proteins from Sheep with Johne's Disease
... Magnetic beads were coated with SurfS1.2 or SurfS2.2 monoclonal antibodies and employed as the primary purification method to isolate target bacterial cells from ovine fecal samples that[r] ...
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Multicolor Upconversion Nanoparticles for Protein Conjugation
... netic beads was placed in a microcentrifuge tube. The magnetic beads were collected with a permanent magnet and washed two times with a hydrogen car- bonate buffer (HCB) solution ...the ...
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Potential Use of DNA Aptamer-Magnetic Bead Separation-PCR Assay for Salmonella Detection in Food
... aptamer-conjugated magnetic beads and incubated for 30 min at ambient temperature with gentle ...ria-aptamer-conjugated magnetic beads were recovered by using the magnetic separation ...
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Combining magnetic nanoparticle with biotinylated nanobodies for rapid and sensitive detection of influenza H3N2
... on magnetic beads to detect the concentration of influenza ...streptavidin magnetic beads. The modified magnetic beads with nanobody capture specifically influenza H3N2 and can ...
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Advances in rare cell isolation: an optimization and evaluation study
... novel magnetic labelling method. The so called dynamic magnetic labelling method yields higher magnetically susceptible target cells (leukocytes) within shorter time when compared to mixing or keeping at ...
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Capturing and concentrating adenovirus using magnetic anionic nanobeads
... polymer-coated magnetic beads from diluted recombinant adenovirus Type ...anionic magnetic beads coated with anionic polymer, ...the beads (BF), supernatant after incubation with the ...
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An Artificial Niche for Circulating Endothelial Cells During Tumor Angiogenesis Mediated by Prostate Cancer Stem Cells
... Fig. 1. Isolation of circulating endothelial cells from peripheral blood: Figure 1A represent Isotype IgG controls for FITC and PE. Figure 1B indicates the percentage purity of CD133 cells isolated using ...
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Comparison of eleven methods for genomic DNA extraction suitable for large-scale whole-genome genotyping and long-term DNA banking using blood samples
... According to the two spectrophotometric ratios, Nucleospin Blood L, Nucleospin Blood XL and Charge Switch Tissue Mini Kits did not extract pure DNA, suitable for NGS or microarray analysis. In the case of Nucleospin ...
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Andes Virus Antigens Are Shed in Urine of Patients with Acute Hantavirus Cardiopulmonary Syndrome
... Hantaviruses have been very difficult to isolate from human sources despite many attempts to do so (53). To date, there is only one report describing a human hantavirus isolate (ANDV isolate CHI-7913) in the Americas ...
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