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Malarial parasite

Advanced Image Analysis based system for Automatic Detection of Malarial Parasite in Blood Images Using SUSAN Approach

Advanced Image Analysis based system for Automatic Detection of Malarial Parasite in Blood Images Using SUSAN Approach

... The objective of the project is to develop a fully automated image classification system to positively identify malaria parasites present in thin blood smears, and differentiate the species. The algorithm generated will ...

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One step concentration of malarial parasite infected red blood cells and removal of contaminating white blood cells

One step concentration of malarial parasite infected red blood cells and removal of contaminating white blood cells

... of malarial parasite-infected red blood cells (PRBCs) that have low contamination of white blood cells (WBCs) facilitates research on the molecular, biochemical and immunological aspects of malarial ...

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Malarial parasite diversity in chimpanzees: the value of comparative approaches to ascertain the evolution of Plasmodium falciparum antigens

Malarial parasite diversity in chimpanzees: the value of comparative approaches to ascertain the evolution of Plasmodium falciparum antigens

... Merozoite surface protein 2 (MSP2) is a highly abundant GPI-anchored protein of P. falciparum that is found exclusively in the Laverania clade [9,40-42]. The msp2 alleles found in the extant P. falciparum populations ...

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A protein centric approach for the identification of folate enzymes from the malarial parasite, Plasmodium falciparum, using OFFGEL™ solution based isoelectric focussing and mass spectrometry

A protein centric approach for the identification of folate enzymes from the malarial parasite, Plasmodium falciparum, using OFFGEL™ solution based isoelectric focussing and mass spectrometry

... Plasmodium falciparum K1 parasite lines were grown at 37°C as described [13,14] in washed O + erythrocytes in RPMI 1640 medium (Invitrogen), supplemented with 0.5% bovine serum albumin, 5 μg/mL hypoxanthine and 50 ...

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Overexpression, purification and assessment of cyclosporin binding of a family of cyclophilins and cyclophilin like proteins of the human malarial parasite Plasmodium falciparum

Overexpression, purification and assessment of cyclosporin binding of a family of cyclophilins and cyclophilin like proteins of the human malarial parasite Plasmodium falciparum

... immunosuppressive effects of cyclosporins have limited their development as antimicrobial agents, but the appearance of non-immunosuppressive derivatives of CsA with pronounced antimalarial activity emphasized the ...

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Use of hydroethidine and flow cytometry to assess the effects of leukocytes on the malarial parasite Plasmodium falciparum.

Use of hydroethidine and flow cytometry to assess the effects of leukocytes on the malarial parasite Plasmodium falciparum.

... inhibit parasite in- vasion, we cocultured the IFN- g -activated or untreated MDMs with schizont-stage parasites for 24 ...minor parasite population with a 10-fold greater mean fluorescence intensity than ...

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Antimitotic herbicides bind to an unidentifed site on malarial parasite tubulin and block development of liver stage Plasmodium parasites

Antimitotic herbicides bind to an unidentifed site on malarial parasite tubulin and block development of liver stage Plasmodium parasites

... Protein affinity assay using the tubulin fusion proteins.. The affinity of the tubulin fusions for other proteins was determined by their ability to.[r] ...

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Automated Diagnosis and Identification of Malarial Parasite in Blood Images

Automated Diagnosis and Identification of Malarial Parasite in Blood Images

... For the study of microscopic blood cell images are captured using digital microscope interface and from thin smear slide after proper staining. It is observed that different image segmentation algorithms can be applied ...

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Structural and statistical aspects in joint modelling of artesunate pharmacometrics and malarial parasite lifecycle

Structural and statistical aspects in joint modelling of artesunate pharmacometrics and malarial parasite lifecycle

... the parasite population never appears to dip and regrow, this may be specified with either parasites in each of the age classes initially, with slower ageing transition times, or with a faster rate of ...

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Plasmodial sugar transporters as anti malarial drug targets and comparisons with other protozoa

Plasmodial sugar transporters as anti malarial drug targets and comparisons with other protozoa

... the malarial parasite is haploid for a greater part of its life cycle (except for the short zygote and ookinete stages inside the mosquito midgut), reverse genetic studies of an essential gene are not ...

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Detection of the Plasmodium falciparumAntigen Histidine Rich Protein 2 in Blood of Pregnant Women: Implications for Diagnosing Placental Malaria

Detection of the Plasmodium falciparumAntigen Histidine Rich Protein 2 in Blood of Pregnant Women: Implications for Diagnosing Placental Malaria

... being malarial parasite negative; ...detected parasite DNA in 65% of the ...tested malarial parasite negative by microscopy were actually infected with low levels of ...

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Biology syllabus grade 12&13.pdf

Biology syllabus grade 12&13.pdf

... Life cycles of parasites , methods of transmission , symptoms of infection and controlling methods • Malarial parasite • Filarial parasite • Hook worm Observation of stages of life cycle[r] ...

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Avian malaria parasites (Plasmodium spp.) in Dunedin and on the Otago Peninsula, southern New Zealand

Avian malaria parasites (Plasmodium spp.) in Dunedin and on the Otago Peninsula, southern New Zealand

... Plasmodium malarial parasites was extracted for use as a positive ...specific malarial parasite (Plasmodium and Haemoproteus) primers L15368 (5’AAA AAT ACC CTT CTA TCC AAA TCT 3’) and H15730 (5’CAT ...

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47. Malaria burden in human population of Khanozai, Pakistan

47. Malaria burden in human population of Khanozai, Pakistan

... of malarial parasites in the human population of Khanozai, ...for malarial parasite. We found higher rate of malarial incidence in males ...of malarial incidence and found more ...

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Restriction fragment length polymorphism mapping of quantitative trait loci for malaria parasite susceptibility in the mosquito Aedes aegypti.

Restriction fragment length polymorphism mapping of quantitative trait loci for malaria parasite susceptibility in the mosquito Aedes aegypti.

... Susceptibility of the mosquito Aedes aegypti to the malarial parasite Plasmodium gallinaceum was investi- gated as a quantitative trait using restriction fragment length poly[r] ...

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Plasmodium falciparum parasite population structure and gene flow associated to anti malarial drugs resistance in Cambodia

Plasmodium falciparum parasite population structure and gene flow associated to anti malarial drugs resistance in Cambodia

... Additional file 1. Geographic distribution of samples and allele frequency. Blood samples from P. falciparum positive patients were collected from 16 health centres or hospitals covering the areas where parasite ...

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Artemisinin resistance containment project in Thailand  II: responses to mefloquine artesunate combination therapy among falciparum malaria patients in provinces bordering Cambodia

Artemisinin resistance containment project in Thailand II: responses to mefloquine artesunate combination therapy among falciparum malaria patients in provinces bordering Cambodia

... ing parasite clearance time rather than any sudden change in therapeutic cure rate ...delayed parasite clearance on day-3 was observed in about 14% of ...delayed parasite clearance time were people ...

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DNA Extraction from Insects by Using Different Techniques: A Review

DNA Extraction from Insects by Using Different Techniques: A Review

... On the other hand, some insect species studied in relation to transmission of dengue virus, like Ades aegypti and A. albopictus. To conduct research on them, their larval colonies established which are time consuming, ...

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PfMDR2 and PfMDR5 are dispensable for Plasmodium falciparum asexual parasite multiplication but change in vitro susceptibility to anti malarial drugs

PfMDR2 and PfMDR5 are dispensable for Plasmodium falciparum asexual parasite multiplication but change in vitro susceptibility to anti malarial drugs

... Background: Membrane-associated ATP binding cassette (ABC) transport proteins hydrolyze ATP in order to translocate a broad spectrum of substrates, from single ions to macromolecules across membranes. In humans, members ...

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Phenotypic Screens Identify Parasite Genetic Factors Associated with Malarial Fever Response in Plasmodium falciparum piggyBac Mutants

Phenotypic Screens Identify Parasite Genetic Factors Associated with Malarial Fever Response in Plasmodium falciparum piggyBac Mutants

... Though the piggyBac system is a powerful tool to study gene function, some factors should be considered in interpreting results. piggyBac methodology introduces a single random disruption per genome. The piggyBac ...

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