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mammalian cells

Spatial and Temporal Analysis of Alphavirus Replication and Assembly in Mammalian and Mosquito Cells

Spatial and Temporal Analysis of Alphavirus Replication and Assembly in Mammalian and Mosquito Cells

... in mammalian cells while establishing noncytopathic, persistent infections in mosquito ...fected mammalian cells, the morphological and structural elements of alphavirus replication and ...

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Broad Tissue and Cell Tropism of Avian Bornavirus in Parrots with Proventricular Dilatation Disease

Broad Tissue and Cell Tropism of Avian Bornavirus in Parrots with Proventricular Dilatation Disease

... cultured mammalian cells in which classical Borna disease virus strains replicate very ...infected cells, suggesting nuclear replication of ...and mammalian bornaviruses is highly con- served ...

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Baculovirus Stimulates Antiviral Effects in Mammalian Cells

Baculovirus Stimulates Antiviral Effects in Mammalian Cells

... insect cells or in any of the over 35 mammalian cell lines studied (15, 21, ...enter mammalian cells, and viral DNA is able to reach the nucleus (9, 21, ...various mammalian ...

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Baculovirus GP64-Mediated Entry into Mammalian Cells

Baculovirus GP64-Mediated Entry into Mammalian Cells

... into mammalian cells through direct interaction with ...Huh7 cells were incubated with the purified GP64 ⌬ TM at 4°C for 1 h, lysed with ice-cold lysis buffer, and subjected to a flotation ...Huh7 ...

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Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P: Implications for FMD Vaccine Development

Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P: Implications for FMD Vaccine Development

... of mammalian cells expressing FMDV P1-3C-SGLuc constructs containing the 3C wild type (WT) or C142T, L127P, or C163A mutants (data represent an average of 7 samples/3C construct ⫾ standard deviations ...

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Small non coding RNAs, mammalian cells, and viruses: regulatory interactions?

Small non coding RNAs, mammalian cells, and viruses: regulatory interactions?

... eukaryotic cells [40] has been debated for ...way, mammalian viruses suppress RNAi by syn- thesizing small viral RNA decoys that competitively occupy RNAi machinery, preventing the processing of authentic ...

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Expression of a recombinant bacterial l asparaginase in human cells

Expression of a recombinant bacterial l asparaginase in human cells

... in mammalian cells was not recognized by patient anti- bodies, while the same biopharmaceutical produced in bacteria and yeast was reactive [18, ...by mammalian cells, after further studies on ...

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Ricin trafficking in plant and mammalian cells

Ricin trafficking in plant and mammalian cells

... in mammalian cells has received little attention to ...the mammalian HRD complex regulator SEL1L (the mammalian equivalent of Hrd3p) has been described for the dislocation of RTA [81], ...

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Membrane topology analysis of HIV 1 envelope glycoprotein gp41

Membrane topology analysis of HIV 1 envelope glycoprotein gp41

... Although the bacterial system is quick and informative, eukaryote specific post-translational modifications and/ or the difference in the composition of lipids in the membrane may affect the topology of gp41. Therefore, ...

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Wongabel Rhabdovirus Accessory Protein U3 Targets the SWI/SNF Chromatin Remodeling Complex

Wongabel Rhabdovirus Accessory Protein U3 Targets the SWI/SNF Chromatin Remodeling Complex

... and mammalian cells and is required for efficient viral ...mosquito cells with WONV, as well as West Nile virus (Flaviviridae) and bovine ephemeral fever virus (Rhabdoviridae), and that SNF5 ...

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Novel Nuclear Export Signal-Interacting Protein, NESI, Critical for the Assembly of Hepatitis Delta Virus

Novel Nuclear Export Signal-Interacting Protein, NESI, Critical for the Assembly of Hepatitis Delta Virus

... Identification of a NES(HDAg-L)-interacting protein by yeast two-hybrid screening. HDAg-L is a nucleocytoplasmic shuttling protein. The proline-rich NES(HDAg-L) mediates the relocalization of HDAg-L to the cytoplasm in ...

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Rheotaxis facilitates upstream navigation of mammalian sperm cells

Rheotaxis facilitates upstream navigation of mammalian sperm cells

... individual cells at zero shear, as the deterministic terms in Figure ...sperm cells to a reversal of the flow direction depends sensitively on ...several cells before/after ...

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Dissecting the Cell Entry Pathway of Baculovirus by Single-Particle Tracking and Quantitative Electron Microscopic Analysis

Dissecting the Cell Entry Pathway of Baculovirus by Single-Particle Tracking and Quantitative Electron Microscopic Analysis

... host cells with the help of actin polymerization and ...infects cells via actin polymerization-dependent internalization and microtubule-dependent nucleocapsid release primarily from within early endosomes ...

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Krüppel like factors in mammalian stem cells and development

Krüppel like factors in mammalian stem cells and development

... these cells: human ESCs are more similar to mouse EpiSCs, which are derived from the late epiblast layer of post-implantation embryos, than to mouse ESCs, which originate from the ICM of blastocysts (Cai et ...

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Wt1 directs the lineage specification of sertoli and granulosa cells by repressing Sf1 expression

Wt1 directs the lineage specification of sertoli and granulosa cells by repressing Sf1 expression

... somatic cells during gonad ...somatic cells will differentiate into granulosa or Sertoli cells in female and male gonads, respectively, with Wt1 ...somatic cells differentiate into ...

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Determination, diversification and multipotency of mammalian myogenic cells

Determination, diversification and multipotency of mammalian myogenic cells

... formative. The complexity of a single mutant phenotype is often such that years of work are required to understand it. For example, after almost ten years since the first report, we still do not know why the Myf5 null ...

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Photoporation and optical manipulation of plant and mammalian cells

Photoporation and optical manipulation of plant and mammalian cells

... The influence of the two biophotonic techniques discussed in this chapter is undeni- able, but only very rarely have they been combined, even though the microscope-based systems generally used for these experiments make ...

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Optical sorting and photo transfection of mammalian cells

Optical sorting and photo transfection of mammalian cells

... specifically cells grown as in vitro cultures, that no two cells are exactly the ...cultured cells can be brought about via a number of changes, including culture passage ...of cells in ...

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A study of vertebrate corticotrophins and related peptides

A study of vertebrate corticotrophins and related peptides

... Several peptides vzith cort icotrophin releasing activity were isolated from posterior pituitary lobe ex­ tracts in the early sixties. A peptide CM-CRF contained all the amino acids of a-MSH plus threonine, alanine and ...

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Norwalk Virus RNA Is Infectious in Mammalian Cells

Norwalk Virus RNA Is Infectious in Mammalian Cells

... Huh-7.5.1 cells or Vero cells, which are defective for IFN production, suggesting that the interferon system does not play a role in the restriction of virus ...Huh-7.5.1 cells, which contain an ...

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