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Mammalian-cells

Small non coding RNAs, mammalian cells, and viruses: regulatory interactions?

Small non coding RNAs, mammalian cells, and viruses: regulatory interactions?

... bottom). Mammalian cells, viruses and small ncRNAs Biological studies on mammalian viruses have illumi- nated aspects of gene regulation by small non-coding RNAs and their RNA-binding ...the ...

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Optical sorting and photo transfection of mammalian cells

Optical sorting and photo transfection of mammalian cells

... the cells of interest are suspended in a solution or mixed with different types of chemicals, biomolecules and ...individual cells in biomedical research, specific cell sub-populations often require ...

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Ricin trafficking in plant and mammalian cells

Ricin trafficking in plant and mammalian cells

... in mammalian cells has received little attention to ...the mammalian HRD complex regulator SEL1L (the mammalian equivalent of Hrd3p) has been described for the dislocation of RTA [81], ...

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Baculovirus Stimulates Antiviral Effects in Mammalian Cells

Baculovirus Stimulates Antiviral Effects in Mammalian Cells

... insect cells or in any of the over 35 mammalian cell lines studied (15, 21, ...enter mammalian cells, and viral DNA is able to reach the nucleus (9, 21, ...various mammalian ...

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Construction of a bacterial autoinducer detection system in mammalian cells

Construction of a bacterial autoinducer detection system in mammalian cells

... We first developed strategies for expressing bacterial LuxR-type proteins that could function as transcription factors in mammalian cells, and the structures of the resultant chimeric proteins described in ...

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Baculovirus GP64-Mediated Entry into Mammalian Cells

Baculovirus GP64-Mediated Entry into Mammalian Cells

... in mammalian cells ...Huh7 cells were treated with various concentrations of M ␤ CD for 30 min, followed by infection with AcCAluc (MOI, 5), NPVpv (MOI, ...Huh7 cells treated with 5 mM M ␤ CD ...

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“Calling Cards” for DNA-Binding Proteins in Mammalian Cells

“Calling Cards” for DNA-Binding Proteins in Mammalian Cells

... ABSTRACT The ability to chronicle transcription-factor binding events throughout the development of an organism would facilitate mapping of transcriptional networks that control cell-fate decisions. We describe a method ...

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Limitations of Allotopic Expression of Mitochondrial Genes in Mammalian Cells

Limitations of Allotopic Expression of Mitochondrial Genes in Mammalian Cells

... Figure 10.—Likelihood of mitochondrial im- port based on a mesohydrophobicity index. (A) A plot developed by Claros and colleagues (Claros 1995) and used to estimate mitochondrial import- ability in yeast. By this ...

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Production of Virus by Mammalian Cells Transformed by Rous Sarcoma and Murine Sarcoma Viruses

Production of Virus by Mammalian Cells Transformed by Rous Sarcoma and Murine Sarcoma Viruses

... Production of Virus by Mammalian Cells Transformed by Rous Sarcoma and Murine Sarcoma Viruses Received for publication 27 November 1967 Cultured cells of mammalian tumors induced by ribo[r] ...

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Mechanisms Involved in Targeted Gene Replacement in Mammalian Cells

Mechanisms Involved in Targeted Gene Replacement in Mammalian Cells

... in mammalian cells, the frequency of this reaction is low and the mechanism ...in mammalian cells using a powerful assay system that permits efficient recovery of the product(s) of individual ...

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CRISPRa-mediated FOXP3 gene upregulation in mammalian cells

CRISPRa-mediated FOXP3 gene upregulation in mammalian cells

... neuronal cells and in mice has recently been rescued by fusing dCas9 to a demethylase TET1, which corrected tran- scriptional regulation of the target FMR1 gene ...in mammalian cells to elucidate ...

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Coatings on mammalian cells: interfacing cells with their environment

Coatings on mammalian cells: interfacing cells with their environment

... the cells with a high density of positive charges for enhanced cell-cell ...incubating cells in PLL, the polycation spe- cies electrostatically adsorbs to the cell surfaces in a speckled cell coating ...the ...

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The oxidative burst reaction in mammalian cells depends on gravity

The oxidative burst reaction in mammalian cells depends on gravity

... Monocyte and macrophage function has shown to be impaired under microgravity conditions for reviews see [4,11,12]. In microgravity substantial changes were de- tected in gene expression of monocytes and in gene induc- ...

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Pseudotyping exosomes for enhanced protein delivery in mammalian cells

Pseudotyping exosomes for enhanced protein delivery in mammalian cells

... Abstract: Exosomes are cell-derived nanovesicles that hold promise as living vehicles for intracellular delivery of therapeutics to mammalian cells. This potential, however, is undermined by the lack of ...

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A heptanucleotide sequence mediates ribosomal frameshifting in mammalian cells.

A heptanucleotide sequence mediates ribosomal frameshifting in mammalian cells.

... The difference in frameshifting efficiency between our reporter gene assay system and the native HIV-1 sequence in vivo and in vitro may be explained by technical differences or by biolo[r] ...

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Enhanced Gene Delivery in Bacterial and Mammalian Cells Using PEGylated Calcium Doped Magnetic Nanograin

<p>Enhanced Gene Delivery in Bacterial and Mammalian Cells Using PEGylated Calcium Doped Magnetic Nanograin</p>

... period. Our results are a fi rst report that introduce the best protocol for increasing the transformation rate. In some groups, the colony number was decreased. This decrease in the colony number in groups 5 and 6 could ...

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The three-dimensional organization of telomeres in the nucleus of mammalian cells

The three-dimensional organization of telomeres in the nucleus of mammalian cells

... Defining the structural organization of the interphase nucleus is, therefore, essential to our understanding of the 3D genome organization in the interphase nucleus. Such a study can be performed by fluorescence in situ ...

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Synthesis and Cleavage of Enterovirus Polypeptides in Mammalian Cells

Synthesis and Cleavage of Enterovirus Polypeptides in Mammalian Cells

... Cell lines anid primary cell cultures examined with regard to viral protein patterns induced by mentgovirus infection" Cell line Primary cell culture origin L-cell HeLa HGS-17 MBK MDCK W[r] ...

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Soluble Antigens of Vaccinia-infected Mammalian Cells

Soluble Antigens of Vaccinia-infected Mammalian Cells

... "Early" refers to "early" virus-induced antigen fraction; "late" refers to "late" virus-induced antigens; G refers to the G antigen components of the "late" antigens obtained by gelfiltr[r] ...

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Interactions of MCP1 with Components of the Replication Machinery in Mammalian Cells

Interactions of MCP1 with Components of the Replication Machinery in Mammalian Cells

... CA). Cells were fixed with 1% formaldehyde and quenching of cross-link was performed with ...glycine. Cells were sonicated six times with a 2-mm tip of a sonicator (Sonics & Mate- rial, ...

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