Mammalian expression plasmids used in this study
Global expression mapping of mammalian genomes
... ones determined for database sequences the error rate for the sequence specific hybridisation must be included in the analysis assumin g that the data are not error free. The amount o f sequence information generated by ...
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Plasmids for Independently Tunable, Low-Noise Expression of Two Genes
... the expression levels of both mRNA and mRNA-binding proteins and by having low cell-to-cell variation in ...was used as a template to develop a fluorescent, IPTG-inducible reporter of expression, ...
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Stable expression plasmids for Streptomyces based on a toxin-antitoxin system
... been used for the positive selection of transformants in ...the expression plasmid and in plasmid-free cells the antitoxin was degraded, leaving the toxin free to exert its effect and causing cell death ...
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Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity
... our study, when pIRES-hrGFPII-r HMGB1 was transfected using the MATra-A transfection protocol, a significantly low PI% and a cell proliferation similar to non-transfected control cells was ...
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Gateways to the FANTOM5 promoter level mammalian expression atlas
... CAGE peaks. Third, it increases the granularity and reus- ability of data as others may use their own method/data to assign gene associations with FANTOM5 CAGE peaks. In modeling the provenance and publication ...
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Microarray analysis of microRNA expression in the developing mammalian brain
... 138 mammalian microRNAs corresponding to the sequences of the microRNAs we cloned as well as to other known ...We used this microarray to determine the profile of microRNAs expressed in the developing mouse ...
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Expression and characterization of a therapeutic monoclonal antibody in mammalian cells
... enhanced expression levels (Ozturk and Hu 2006; Li et ...be used, as for example the ability to grow in serum-free medium or the resistance to the stress and shear conditions found in bioreactors (Lloyd et ...
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Regulation of Mammalian Mitochondrial Gene Expression: Recent Advances
... the study of patients with respiratory chain ...gene expression is expected to continue focusing on the regulatory function of post-transcriptional mtRNA modi fications as well as on the biogenesis of the ...
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Supplemental Fig. S1. The schematic diagrams of the expression constructs used in this study.
... or mutant Dbn1. For each condition, the EPSC amplitude, which was normalized to baseline prior to LTD induction, was plotted as mean ± SEM. Two-tailed Student’s t-test was used for statistical analysis. n= 9-10 ...
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In silico cloning and bioinformatics study of Brucella melitensis Omp31 antigen in different mammalian expression vectors
... transient expression in mammalian ...proper expression of the recombinant ...an expression vector designed for high level expression and secretion of foreign proteins in mamma- lian ...
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A computational study of the mammalian subiculum
... isoform expression between and within cell ...exogenous expression systems are shifted positively or negatively depending on the combination of co-expressed subunits (Qu, Curtis, et ...
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2 The abbreviations used are: DM, diabetes mellitus; mtor, mammalian target
... present study, transferrin was also increased in the rapamycin-treated diabetic ...increased expression of the antioxidant enzymes as well as iron- regulating proteins in the diabetic ...
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Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis
... assumption, plasmids pHT01-bgaB (control), and four inducer-free expres- sion vectors containing Pgrac01 promoter, pHT1655, pHT2071, pHT1660 and pHT1663 were transformed into ...We used the bgaB gene coding ...
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Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
... we used the Kanamycin resistance gene and a styA-EGFP styB (AEB) expression cassette as a cargo module that was already described previously in stud- ies using Pseudomonas [7, ...was used here as a ...
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Simplified ontologies allowing comparison of developmental mammalian gene expression
... this study, we searched for human-mouse orthologs that are expressed in the normal postnatal and developmental brain of both spe- cies, where a gene is classified as normal if its originating library was annotated ...
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IRFinder: assessing the impact of intron retention on mammalian gene expression
... Conclusions In this study we developed bioinformatics tools to study the impact of intron retention on gene regulation. Be- cause of their length and low complexity, intron expres- sion is difficult to ...
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The contributions of normal variation and genetic background to mammalian gene expression
... gene expression variability observed in genetically identical ...this study, and we did not observe any histo- logical differences in the cellular composition of livers within or between ...gene ...
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Simplified ontologies allowing comparison of developmental mammalian gene expression
... this study, we searched for human-mouse orthologs that are expressed in the normal postnatal and developmental brain of both spe- cies, where a gene is classified as normal if its originating library was annotated ...
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Single mammalian cell gene expression analysis using microfluidics
... to study multiplexed gene expression in single mammalian cells, as well as to determine phenotypes of unculturable bacteria found in the termite ...of mammalian cells, single cells are seeded ...
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Developmental gene expression differences between humans and mammalian models
... We used this expression atlas of human organ development to test for associations between the spatiotemporal profiles of hu- man disease genes and the etiology and phenotypic manifesta- tion of human ...
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