MARC-145
Inhibition of highly pathogenic PRRSV replication in MARC 145 cells by artificial microRNAs
... Plasmids or adenoviruses delivery strategy is limited by its transient nature, as a result, RNAi effects were reduced over time post-infected with PRRSV and PRRSV could replicate afterwards. Also, PRRSV might develop ...
11
Porcine reproductive and respiratory syndrome virus inhibits MARC 145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway
... in MARC-145 cells infected by PRRSV was confirmed with immuno- precipitation assay using Cdc2 antibody to precipitate p21 ...infected MARC-145 ...PRRSV-infected MARC-145 cells, ...
15
Identification of nonessential regions of the nsp2 protein of an attenuated vaccine strain (HuN4 F112) of highly pathogenic porcine reproductive and respiratory syndrome virus for replication in marc 145 cell
... Finding: In this study, the nonessential regions of the nsp2 of an attenuated vaccine strain (HuN4-F112) of highly pathogenic porcine reproductive and respiratory syndrome virus were identified based on an infectious ...
7
Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC 145 cell line
... infected MARC-145 cells ...PRRSV-infected MARC-145 cells (Figure 1, data not shown, ...infected MARC-145 cells and the absence of its up regulation in infected SJPL cells (Figure ...
14
Porcine reproductive and respiratory syndrome virus (PRRSV) infection spreads by cell to cell transfer in cultured MARC 145 cells, is dependent on an intact cytoskeleton, and is suppressed by drug targeting of cell permissiveness to virus infection
... [24]). MARC-145 cells were cultured and inoculated with PRRSV in glass-bottom slide chambers, fixed in 80% acetone, and incubated for 1 h at 37°C with a 1:100 dilution of FITC-conjugated SDOW17 antibody ...
17
Modulation of CD163 Expression by Metalloprotease ADAM17 Regulates Porcine Reproductive and Respiratory Syndrome Virus Entry
... Metalloprotease ADAM17 inhibition assay. To investigate the role of ADAM17 in altering PRRSV entry by regulating viral cellular receptors, such as CD163 and CD169, we used batimastat (BB94; Sigma-Aldrich, St. Louis, MO) ...
12
Carbon Monoxide Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication by the Cyclic GMP/Protein Kinase G and NF-κB Signaling Pathway
... CO. MARC-145 cells were pretreated with the CO scavenger Hb (50 g/ml) for 1 h; after the cells were washed with phosphate-buffered saline (PBS) three times, they were infected with PRRSV at an MOI of ...
19
Evaluation of the Efficacy of an Attenuated Live Vaccine against Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus in Young Pigs
... Since May 2006, a new highly pathogenic porcine reproductive and respiratory syndrome has been epizootic in major pig-pro- ducing areas of China. Studies have confirmed that the causative agent of this clinical outbreak ...
8
Increasing Expression of MicroRNA 181 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication and Has Implications for Controlling Virus Infection
... PRRSV-infected Marc-145 cells, the viral mRNAs could compete with miR-181-targeted sequence in luciferase vector to interact with miR-181 and result in less inhibition of luciferase activity, further ...
13
14-3-3ε acts as a proviral factor in highly pathogenic porcine reproductive and respiratory syndrome virus infection
... in Marc-145 cells and porcine alveolar macrophages (PAMs) caused a significant decrease in TA-12 replication, while stable overexpression of 14-3- 3ε caused a significant increase in the replication of ...
11
Porcine Reproductive and Respiratory Syndrome Virus Inhibits Type I Interferon Signaling by Blocking STAT1/STAT2 Nuclear Translocation
... 33). MARC-145 cells, in the presence or ab- sence of PRRSV infection, were treated with IFN- ␣ for 8 h and harvested for Western ...mock-treated MARC-145 ...
12
Cellular Poly(C) Binding Proteins 1 and 2 Interact with Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 1β and Support Viral Replication
... transfection, MARC-145 cells were infected with FL12 at an MOI of 1 for 12 h, after which the cells were collected and total RNA was ...(B) MARC-145 cells treated with siRNAs as shown on top ...
11
A Subset of Porcine Reproductive and Respiratory Syndrome Virus GP3 Glycoprotein Is Released into the Culture Medium of Cells as a Non-Virion-Associated and Membrane-Free (Soluble) Form
... Therefore, data showed that during PRRSV infection of MARC-145 cells, a fraction of GP3 about 10 to 20% of the amount of GP3 immunoprecipitated from PRRSV-infected cell lysates was able [r] ...
9
Dollar Marc Hanna 1896 election info Democrats lose Republican Dominance
... Dollar Marc Hanna Dollar Marc Hanna 1896 election info 1896 election info Democrats lose Democrats lose.. Republican Republican Dominance1[r] ...
31
Dual-strand tumor-suppressor microRNA-145 miR-145-5p and miR-145-3p ) coordinately targeted MTDH in lung squamous cell carcinoma
... Patients with lung adenocarcinoma may benefit from recently developed molecular targeted therapies. However, analogous advanced treatments are not available for patients with lung squamous cell carcinoma (lung SCC). The ...
15
From the laboratory to the patient and back an interview with Marc Mareel
... of Marc Mareel is a synthesis of scientific research and clinical ...retirement, Marc Mareel has continued to integrate clinical practice with research ...
7
P< 0.05). Also, the group D had lower cell proportion and cell apoptosis rate than the
... Abstract: Objective: This study was undertaken to probe deeply into effects of microRNA-145 (miR-145) expression on chondrocyte apoptosis in osteoarthritis (OA). Methods: A group of 71 OA patients (32 males ...
7
Revue du Marche Commun no 145 = Review of the Common Market July August 1971 No 145
... Cette so·Jution résulte d'ailleurs nécessairement de la confrontation entre l'article 92 et 1 qui déc:Jare « incompatibles avec le Marché Commun dans •la mesure où e'J.les affectent 1les[r] ...
60
A brief intervention to reduce offending : the study of a faith based programme : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Psychology at Massey University
... conduct. MARC is a brief, faith-based, biblical approach to curtailing offending, developed at Tauranga Community Probation Service between 1993 and ...10-session MARC course, participants were asked to ...
13
Regulatory Mechanism of MicroRNA 145 in the Pathogenesis of Acute Aortic Dissection
... Secondly, miR-145 mimic or inhibitor was utilized to elicit over- expression or silencing of miR-145 expression in VSMCs. As we expected, miR-145 expression was significantly increased in VSMCs ...
8