Mass spectrometry analysis and database search
A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.
... vivo. Mass spectrometry (MS) is typically the method of choice for high-throughput detection of protein post-translational modifications (PTMs) because a moiety covalently bound to a peptide can be directly ...
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Evaluation of database search programs for accurate detection of neuropeptides in tandem mass spectrometry experiments
... 50 X!Tandem and Crux were 78%, 63% and 52%, respectively. This result also indicates that the selected threshold was more stringent in Crux and X!Tandem than for OMSSA. The statistical significance of the X!Tandem ...
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Database Search Strategies for Proteomic Data Sets Generated by Electron Capture Dissociation Mass Spectrometry
... The search engines that have been employed for large-scale ECD data analysis are Mascot and ...These search engines have certain limitations, for example, the product ion tolerance cannot be ...
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Phosphorylation Analysis by Mass Spectrometry
... Refs. 7–9). However, when following their line of argument that the phosphorylated form of a particular peptide should have lower ionization/detection efficiencies than their unmod- ified cognate because both the ...
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Pesticide Analysis by Mass Spectrometry
... Q12. Why was chrysene-d 12 selected as an internal standard rather than chrysene with a natural isotopic abundance of hydrogen nuclei? Would you expect that the chrysene and chrysene-d 12 to be resolved in the GC ...
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Drug analysis by mass spectrometry
... Plasma morphine concentrations in four patients with renal failure and in three healthy volunteers were measured using CI QSIM, and morphine pharmacokinetics in th[r] ...
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LIBRARY SEARCH-BASED DRUG ANALYSIS IN FORENSIC TOXICOLOGY BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY
... Triple-quadrupole mass spectrometers have widely been used for quantitative analysis because of their high selectivity and sensitivity combined with good ...
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LipidBlast in silico tandem mass spectrometry database for lipid identification.
... predict mass spectral peak intensities using peak intensities from experimentally measured spectra 18 ...of mass spectral peak abundances given a compound structure only 1 ...different mass ...
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ANALYSIS OF PROTEINS AND PROTEOMES BY MASS SPECTROMETRY
... biomolecules, mass spectrometry has become a powerful tool in protein analysis and the key technology in the emerging field of ...of mass spectrom- etry is driven both by innovative ...
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Mass Spectrometry Analysis of Cancer Biopsies
... using mass spectrometry-based proteomics. However, with mass-limited samples like tumor biopsies, it can be challenging to obtain sufficient amounts of proteins to generate high-quality mass ...
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Surface analysis for proteomics via liquid extraction surface analysis mass spectrometry and liquid chromatography mass spectrometry
... peptide analysis for several ...available mass spectrometers such as ion traps, orbitraps and quadrupoles have upper m/z limits of around 2000 ...require analysis of complex mixtures of proteins and ...
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Chromatography–mass spectrometry analysis of tianeptine in urine
... chromatography–mass spectrometry. Methods: The analysis has been carried out on a gas chromatograph Agilent 6890N with a mass- selective detector Agilent 5973 and an injector Agilent 7683 ...
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Additives for improved analysis of lipids by mass spectrometry
... List of Figures Figure 1 A simplified schematic of the MALDI process. Matrix/analyte crystals deposited on a MALDI target plate are ablated with a pulsed laser. Energy from the laser is absorbed by matrix molecules and ...
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Statistical analysis of proteomic mass spectrometry data
... The breast cancer and melanoma datasets were studied in chapter 3 using a Bayesian approach which utilised MCMC algorithms. This approach modelled the spectra as a series of peaks with differing heights and locations. ...
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YPED: An Integrated Bioinformatics Suite and Database for Mass Spectrometry-based Proteomics Research
... resulting mass spectrometric proteomics ...local database and a public repository that provides access to published and anonymous ...and search results stored in the database can be queried ...
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Liquid Chromatography - Quadrupole Time-of-Flight Mass Spectrometry Analysis of Disperse Dyes for the Development of a Forensic Dye Database.
... 15 in the microscope and a beam infrared light passes through the fiber, reflects off the metal surface, and passes through the fiber a second time. 20 Flattening fibers for analysis reduces the lensing effect, ...
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Mass Spectrometry: Quadrupole Mass Filter
... 5. Now you are ready to operate the QMS and take spectra. Suggested Experiments 1. Residual Gas Analysis. Take a scan of the residual gas inside the vacuum chamber at a pressure of 10 -6 Torr or lower. The log ...
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chromatography mass spectrometry
... pharmaco- mass dose (for metabolism studies) or a 1-mg / kg logical profile was compared to that of 1-[3-chloro- body mass dose (for STA) in aqueous suspension by phenyl]piperazine (mCPP), an active ...
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Challenges and opportunities of bovine milk analysis by mass spectrometry
... of mass spectrometric (MS)-based techniques is steadily improving our understanding of the complexity of the biological traits that effect milk yield and its components to meet the global demand arising from ...
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Analysis of posttranslational modifications of proteins by tandem mass spectrometry
... initial database search is typically performed with only a limited set of specified modifications, including alkylated cysteines and oxidized methi- onines, in order to minimize search space and to ...
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