Mass spectrometry based proteomics
Untargeted analysis of the airway proteomes of children with respiratory infections using mass spectrometry based proteomics
... a mass spectrometry based proteomics method for characterizing the upper airways ...UTM based on the number of proteins identified without BSA depletion (Total proteome: Protocol A) and ...
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Mass Spectrometry-Based Proteomics Reveals Distinct Mechanisms of Astrocyte Protein Secretion
... in mass spectrometry-based proteomics have enabled greater depth of analysis for the astrocyte secretome, allowing comparative analyses of secretomes under different conditions and the ...
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Review Article Mass Spectrometry-Based Proteomics and Peptidomics for Biomarker Discovery in Neurodegenerative Diseases
... several mass spectrometry methods have been employed for the characterization/validation phase of a clinical diagnostic ...quadrupole mass spectrometer has been employed for biomarker quantitation ...
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Mass spectrometry-based proteomics techniques and their application in ovarian cancer research
... The improvement in –omics sciences, genomics, proteo- mics, metabolomics, has opened a new research chapter, which is expected to develop novel tools for early diagno- sis, treatment monitoring or population screening ...
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New Reagents and Methods for Mass Spectrometry-based Proteomics Investigations
... ABSTRACT We present novel homobifunctional amine-reactive clickable cross-linkers (CXLs) for investigation of three-dimensional protein structures and protein-protein interactions (PPIs). CXLs afford distinct advantages ...
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Mass Spectrometry-Based Proteomics Analysis of the Matrix Microenvironment in Pluripotent Stem Cell Culture
... conclusions based on changes in cellular activity when using Matrigel™ ...was based on the detection of several growth factors in standard Matrigel™ through the use of immunoassays; these included bFGF, ...
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Discovery and longitudinal evaluation of candidate biomarkers for ischaemic stroke by mass spectrometry-based proteomics
... used. Based on the analysis of the serum sam- ple collected immediately after the completion of the imaging (baseline when CTp was used and T0 when MRI was ...
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Contributions of mass spectrometry based proteomics to defining cellular mechanisms and diagnostic markers for systemic lupus erythematosus
... Mass spectrometry-based proteomic technologies have played an important role in each area of clinical diagnosis as well as the development of a more comprehensive understanding of the underlying ...
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Novel applications of mass spectrometry-based proteomics in clinical microbiology
... van proteomics analyses met behulp van massaspectrometrie, de aanwezigheid van de corresponderen eiwitten snel en accuraat konden ...bottom-up proteomics, waarbij eiwitten in eerste instantie worden ...
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Application of label free mass spectrometry based proteomics to biomarker discovery
... Double centrifuged maternal plasma samples were supplied by Prof. Kypros Nicolaides of King’s College Hospital, London, UK with full ethical approval. Forty maternal plasma samples were selected for this part of the ...
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A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study
... analyze mass spec- trometry-based isotope-labeled datasets, targeting the two questions that typically arise in proteomics experi- ments: 1) which proteins are differentially regulated regarding the ...
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Studying epigenetic complexes and their inhibitors with the proteomics toolbox
... that proteomics can and will help on every step of the epigenetic drug discovery effort in the years to ...using proteomics ap- proaches lie ahead of ...the proteomics toolbox to study drug action ...
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Investigation of post-translational modifications in type 2 diabetes
... Mass spectrometry based proteomics technology has provided an opportunity to study proteins, their PTMs like phosphorylation, acetylation, N-glycosylation, O-GlcNAcylation and Advanced ...
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Recent advances in mass spectrometry based clinical proteomics: applications to cancer research
... limitations. Mass spec- trometry (MS) is a powerful method that enables increasingly comprehensive insights into changes of the proteome to advance personalized ...clinical proteomics are high- lighted with ...
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Mass spectrometry-based approaches to targeted quantitative proteomics in cardiovascular disease
... ApoA-I is the major protein constituent of high density lipoprotein (HDL) particles, accounting for approxi- mately 65 % of the protein mass [16]. It is a 45.4 kDa pro- tein that is composed of 396 amino acids. ...
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Mass spectrometry-assisted gel-based proteomics in cancer biomarker discovery: approaches and application
... Because of the limited availability and accessibility of suitable reagents, most proteins in a species cannot be detected and quantified by affinity-based assays [17]. Therefore, almost all currently available ...
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Microbial proteomics: a mass spectrometry primer for biologists
... In order to acquire structural or peptide sequence infor- mation, it is necessary to induce fragmentation of the pep- tides of interest. This is not possible with soft ionisation techniques such as ESI and MALDI, ...
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Current applications of proteomics: a key and novel approach
... fluids. Proteomics based technical knowledge is applied and verified in several preclinical research settings such as invention of diagnostic markers for specific disease and have shown to be increased in ...
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Advances in mass spectrometry-based clinical biomarker discovery
... shotgun proteomics in the develop- ment of clinical biomarkers should not dissuade aspiring clinical chemists or other scientists involved in biomarker discovery from adopting mass spectrometry ...
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Quantification of mutant SPOP proteins in prostate cancer using mass spectrometry based targeted proteomics
... The matrix effect on all the 16 target peptides was fur- ther investigated by plotting the peptide recovery (peak area ratio of no-matrix versus with-matrix) to their theo- retical hydrophobicity calculated based ...
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