matrix metalloproteinase 13
Activating enhancer binding protein 2 epsilon (AP 2ε) deficient mice exhibit increased matrix metalloproteinase 13 expression and progressive osteoarthritis development
... found matrix metalloproteinase 13 (Mmp13), a major mediator of cartilage destruction, to be significantly upregulated in articular cartilage of adult Tfap2e − / − ...extracellular matrix ...
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Matrix metalloproteinase-13 refines pathological staging of precancerous colorectal lesions
... An exact classification of precancerous stages of colorectal polyps might improve therapy and patients´ outcome. Here we investigate the association between grade of dysplasia and Matrix ...
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Original Article Expression of matrix metalloproteinase-8 and matrix metalloproteinase-13 in mast cells of human periapical lesions
... against MMP-8 (Boster) and MMP-13 (Boste- rz) at a 1:100 dilution. Secondary antibodies were goat anti-mouse IgG (H + L) Alex Flour 555 and goat anti-rabbit (H + L) Alex Flour 488 (Cell Signaling Technology), at a ...
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Cloning, expression, and type II collagenolytic activity of matrix metalloproteinase 13 from human osteoarthritic cartilage
... The RT-PCR data on expression of MMP-13 in cartilage were confirmed using Northern blot analysis. Cartilage from three out of six osteoarthritic knee joints expressed readily de- tectable levels of both MMP-1 and ...
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Original Article The expression levels of interleukin-6 (IL-6), IL-12, and matrix metalloproteinase-13 (MMP-13) in the synovial fluid and synovium in hip osteoarthritis and their relationship with disease progression
... degree of articular cartilage destruction [16]. After the expression of IL-6 is inhibited, the severity of osteoarthritis in model mice is sig- nificantly reduced [21]. IL-12 is produced by activated macrophages, ...
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Human articular chondrocytes produce IL 7 and respond to IL 7 with increased production of matrix metalloproteinase 13
... of matrix-degrading enzymes, as we had previously shown using fibronectin frag- ment ...MMP- 13 production was confirmed by ELISA using additional chondrocyte cultures (Figure ...
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Adiponectin may contribute to synovitis and joint destruction in rheumatoid arthritis by stimulating vascular endothelial growth factor, matrix metalloproteinase 1, and matrix metalloproteinase 13 expression in fibroblast like synoviocytes more than proinflammatory mediators
... Real-time quantitative polymerase chain reaction (PCR) was carried out using the LightCycler PCR system (Roche Diag- nostics, Meylan, France) using the DNA binding SYBR Green I dye to detect the PCR products as described ...
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The triterpenoid CDDO inhibits expression of matrix metalloproteinase 1, matrix metalloproteinase 13 and Bcl 3 in primary human chondrocytes
... An alternative approach to the prevention of cartilage degradation is the inhibition of MMPs by targeting either the expression of their genes or the synthesis of the pro- teins. Triterpenoids are a novel family of ...
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Original Article Toll-like receptor 4 affects the expression of matrix metalloproteinase 13 in human chondrocytes
... pendent pathways [25, 26]. Nuclear Factor-κB (NF-κB) is located downstream of these two pathways. Once the NF-κB is activated, numer- ous pro-inflammatory cytokines are produced such as TNF-α [27]. Additionally, the ...
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Altered endochondral bone development in matrix metalloproteinase 13 deficient mice
... of matrix components in maintaining structural integrity and function of cartilage, as well as the role of the cartilage matrix in the differentiation process of its cellular components is well ...cartilage ...
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Serum and saliva collagenase-3 (MMP-13) in patients with oral lichen planus and oral squamous cell carcinoma
... the matrix me- thaloproteinases (MMPs) ...ogenesis. Matrix metalloproteinase-13 (MMP-13) or collagenase-3 was originally cloned from a breast tumor and cleaves preferentially type II ...
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Decrease in the expression of the type 1 PTH/PTHrP receptor (PTH1R) on chondrocytes in animals with osteoarthritis
... like matrix metalloproteinase 13 (MMP-13) who have been proven to be the principal initiator of OA progression were significantly up-regulated in sclerotic osteoblasts compared with ...
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Curcumin slows osteoarthritis progression and relieves osteoarthritis associated pain symptoms in a post traumatic osteoarthritis mouse model
... Ibex), matrix me- talloproteinase (MMP)-13 (Abcam), and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)5 (Abcam) followed by incubation with anti-mouse or anti-rabbit ...
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Expression of CD147 and matrix metalloproteinase 11 in colorectal cancer and their relationship to clinicopathological features
... lular matrix metalloproteinase inducer (EMMPRIN), is a member of the immunoglobulin superfamily of adhesion ...secrete matrix metalloproteinases ...
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The matrix metalloproteinase and insulin-like growth factor system in oral cancer – a prospective clinical study
... Sandwich enzyme-linked immunosorbent assay (ELISA) was used for the quantification of MMP-2, MMP-3, and MMP-13 levels in blood samples with the DuoSets ELISA for humans (R&D Systems, Minneapolis, MN, USA) ...
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Original Article Immunohistochemical expression of matrix metalloproteinase-9 and inhibitor of matrix metalloproteinase-1 in prostate adenocarcinoma
... the matrix metalloproteinase-9 and the inhibitor of matrix metalloproteinase-1 was carried out at the benign prostatic hyperplasia (BPH) and the adenocarcinoma (AC) of different Gleason’s ...
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Multiple myeloma–derived MMP 13 mediates osteoclast fusogenesis and osteolytic disease
... genitors into multinucleated OCLs that likewise correlated with lower levels of NFATc1 and DC-STAMP expression. With regard to uncovering the MMP-13–dependent signaling cascades that underline OCL progenitor ...
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Matrix metalloproteinase 28, a novel matrix metalloproteinase, is constitutively expressed in human intervertebral disc tissue and is present in matrix of more degenerated discs
... and metalloproteinase and in a disintegrin and metalloproteinase with thrombospondin) or the transmembrane group as found in membrane-type MMPs [13], and the MMP28 promotor has a unique conserved ...
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Serum Matrix Metalloproteinase 3 and Tissue Inhibitor Metalloproteinase 1 in Vascular Dementia: A Comparative Study
... of matrix metalloproteinase 3 (MMP3) and tissue inhibitor metallo- proteinase 1 (TIMP1) in vascular dementia patients and healthy control ...of matrix metalloproteinase 3 (MMP3) and tissue ...
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Taurine chloramine differentially inhibits matrix metalloproteinase 1 and 13 synthesis in interleukin 1β stimulated fibroblast like synoviocytes
... TauCl differentially inhibited the expression of MMPs in IL-1β stimulated RA FLSs. The expression of MMP-13 was significantly inhibited at concentrations of 400 to 600 μmol/l TauCl, whereas the expression of MMP-1 ...
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