MCF-10A
Morphologic transformation of human breast epithelial cells MCF-10A: dependence on an oxidative microenvironment and estrogen/epidermal growth factor receptors
... samples containing 42 μg protein were electrophoresed onto two 12% SDS-Tris-HCl polyacrylamide gels (gel 1: Fig. 7a, c, e & gel 2: Fig. 7b, d, f) and transferred to nitrocellulose membranes. The two membranes were ...
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A novel MCF-10A line allowing conditional oncogene expression in 3D culture
... subline, MCF-10AecoR (a kind gift of ...[14,25,44,46-48]. MCF-10Atet cells were generated by transfecting MCF-10AecoR cells with the AhdI-linearized expression vector pWHE644, which uses the EF-1 a ...
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Comparison of cytotoxicity and genotoxicity of 4 hydroxytamoxifen in combination with Tualang honey in MCF 7 and MCF 10A cells
... lines, MCF-7 and MDA-MB-231, were highly sensitive to TH while the non-cancerous MCF-10A breast epithelial cells were not affected ...on MCF-7 and MCF-10A cells and to determine ...
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Activation of the Farnesoid X-receptor in breast cancer cell lines results in cytotoxicity but not increased migration potential
... (i.e. MCF-10A), receptor positive tumour (i.e. MCF-7) and triple negative tumours ...for MCF-7 and MDA-MB-231 cells that the FXR- dependent signalling cascade is intact, with activation of SHP ...
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Association between promoter polymorphisms of matrix metalloproteinase-1 and risk of gastric cancer
... by breast cancer cells and correlate with collagen degradation, trans- formation-like morphological changes in MCF-10A acini, and tumor aggressiveness. Biomed Res Int. Decock J, Hendric[r] ...
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Original Article Aberrant expression of receptor tyrosine kinase EphA7 in breast cancers
... line MCF-10A, breast cancer cell lines MCF-7, MDA-MB-231, SK-BR-3 and in a set of 150 invasive ductal cancer specimens by using immunohistochemical staining with an EphA7 specific polyclonal anti- ...
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Arsenic, cadmium and neuron specific enolase (ENO2, γ-enolase) expression in breast cancer
... the MCF-10A cell line was in agreement with the immunostaining results found in the normal ...the MCF-10A cell line had a high level of ENO1 expression in both mRNA and ...the ...
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Original Article MiR-486-5p prevents migration, invasion and EMT by regulating Smad2 in breast cancer
... line MCF-7 and the non-malignant breast epithelial cell (MCF-10A) was detected by ...in MCF-7 ...repressed MCF-7 cell proliferation, migration, invasion and ...in MCF-7 ...on ...
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The small molecule C 6 is selectively cytotoxic against breast cancer cells and its biological action is characterized by mitochondrial defects and endoplasmic reticulum stress
... in MCF-7, T47D, and MDA-MB-231 cells upon treatment with 30 μM C-6 but not in MCF-10A cells (Figure 2A and Additional file ...morphology. MCF-10A and MCF-7 ...
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The combinatorial activation of the PI3K and Ras/MAPK pathways is sufficient for aggressive tumor formation, while individual pathway activation supports cell persistence
... the MCF-10A cells do not significantly grow in minimal assay media, they also do not die (Figures 1B and S2); therefore serum was removed to further stress the cells for the apoptosis ...However, ...
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Original Article MiR-1228 promotes breast cancer cell growth and metastasis through targeting SCAI protein
... MicroRNAs can modulate a wide variety of bio- logical processes. It has been demonstrated that miRNAs can play specific roles in cancer cell proliferation, differentiation, migration and metastasis. Numerous miRNAs have ...
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Traditional Chinese medicinal formula Si-Wu-Tang prevents oxidative damage by activating Nrf2-mediated detoxifying/antioxidant genes
... cells. Expression of Nrf2 and Nrf2-regulated genes HMOX1 (heme oxygenase 1) and SLC7A11 (xCT) was evaluated by quantitative RT-PCR, Western Blot and immunocytochemistry. SWT strongly induced Nrf2-regulated genes at mRNA ...
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(6E, 10E) Isopolycerasoidol and (6E, 10E) isopolycerasoidol methyl ester, prenylated benzopyran derivatives from pseuduvaria monticola induce mitochondrial mediated apoptosis in human breast adenocarcinoma cells
... Phytochemicals from Pseuduvaria species have been reported to display a wide range of bi- ological activities. In the present study, a known benzopyran derivative, (6 E ,10 E ) isopoly- cerasoidol (1), and a new ...
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Journal of Applied Pharmaceutical Science
... Modulation of CYP1B1 and CYP1A1 gene expression and activation of aryl hydrocarbon receptor by Ginkgo biloba extract in MCF-10A human mammary epithelial cells. Ginkgo biloba[r] ...
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Utilization of adipocyte-derived lipids and enhanced intracellular trafficking of fatty acids contribute to breast cancer progression
... epithelium MCF-10A cells (provided by the Key Labora- tory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming) were ...
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Original Article Effects of Scribble on mammary tumorigenesis and metastasis in breast cancer
... Fresh breast cancer tissue samples were lysed in lysis buffer RIPA (Roche, Swiss). After cen- trifugation (10 min, 44°C, 16,000 rpm), super- natants were collected and assayed for total protein content using BCA kit ...
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FOXF2 deficiency promotes epithelial mesenchymal transition and metastasis of basal like breast cancer
... line MCF-10A and the invasive BLBC cell lines MDA-MB- 231 was selected to establish stable FOXF2-knockdown cells by infection with a lentiviral vector expressing a FOXF2 shRNA (shFOXF2) or a non-target ...
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Type I insulin like growth factor receptor over expression induces proliferation and anti apoptotic signaling in a three dimensional culture model of breast epithelial cells
... MCF-10A cells were obtained from the American Type Tissue Culture Collection and cultured in F12/DMEM (Gibco, Invitro- gen Corp., Carlsbad, CA, USA) with 5% horse serum (Gibco), 5 ng/ml epidermal growth ...
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An Active Form of Vav1 Induces Migration of Mammary Epithelial Cells by Stimulating Secretion of an Epidermal Growth Factor Receptor Ligand
... of MCF-10A ...of MCF-10A cells expressing GFP is shown in the left panels and of cells expressing Vav1Y3F is shown in the right ...(C) MCF-10A cells expressing GFP and Vav1Y3F ...
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Dynamics and mechanisms of quantum dot nanoparticle cellular uptake
... In stark contrast to the rapid and large intracellular uptake of QD655-COOH, no detectable uptake was observed for either amine-PEG or PEG coated QDs. Sim- ilar findings have been reported for QD cellular uptake by HEK ...
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