MDCK cells
Comparative Study of Influenza Virus Replication in MDCK Cells and in Primary Cells Derived from Adenoids and Airway Epithelium
... antibody-secreting cells, and cytokine responses suggested that ca A/Leningrad/134/17/57 is a superior immuno- gen to ca A/Leningrad/134/47/57, which in turn is superior to ca A/Ann Arbor/6/60 ...lines, ...
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Effect of human activated NRAS on replication of delNS1 H5N1 influenza virus in MDCK cells
... in MDCK cells influenced cell morphology and cell-cell contacts, lead- ing to the production of comet ...the cells was not ...mouse cells, a transient increase in the cytoplasmic level of ...
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Protective essential oil attenuates influenza virus infection: An in vitro study in MDCK cells
... MDCK cells were exposed to oil-treated or untreated PR8 at an MOI of ...infection, cells were exposed to sterile virus-free ...from MDCK cells was extracted using TRIzol (Invitrogen, ...
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Passive potassium fluxes in cultural HeLa and MDCK cells
... Identical experiments on MDCK cells are shown in figures 3.13a and 3.13b. The order of potency for the three diuretics is similar to the HeLa data. Bumetanide (apparent Ki of 0.25;aM) is 10.6 times more ...
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Reovirus-induced apoptosis of MDCK cells is not linked to viral yield and is blocked by Bcl-2.
... in MDCK cells overexpressing Bcl-2 are significantly less than those in control cells ...in MDCK cells by Bcl-2 overexpression does not limit reovirus growth to the same extent as that ...
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MDCK SIAT1 Cells Show Improved Isolation Rates for Recent Human Influenza Viruses Compared to Conventional MDCK Cells
... of MDCK-SIAT1 cells com- pared to conventional MDCK cells for isolation of influenza virus from stored human clinical samples was ...use MDCK-SIAT1 or the similar cell line ST6Gal I ...
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Growth Determinants for H5N1 Influenza Vaccine Seed Viruses in MDCK Cells
... (A) MDCK cells were infected with PB2 mutant virus at an MOI of ...with MDCK cells at 36 ...into MDCK cells by using plasmids expressing PB1, PA, and NP of PR8(UW) plus a ...
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Chitosan encapsulation modulates the effect of capsaicin on the tight junctions of MDCK cells
... the cells were no longer able to recover to their original TEER value after 24 h, and at even higher doses of capsaicin the TEER values flattened out to ...
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Occluding junctions and paracellular pathways studied in monolayers of MDCK cells
... This study indicated that: 1 in monolayers of MDCK celk current flows through a paracellular route, but 2 conductance may have large variations along the perimeter of a given cell, and t[r] ...
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Control of microtubule assembly in 'Drosophila' and MDCK cells
... Microtubules of the mitotic spindle are apparently far more dynamic than those of the interphase microtubule array.. For example, fluorescence photobleaching recovery indicated that.[r] ...
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Virions and intracellular nucleocapsids produced during mixed heterotypic influenza infection of MDCK cells.
... Lane 1, Mock-infected cells; lane 2, cells infected with A/WSN/33; lane 3, cells doubly infected 1:1 with A/WSN/33 and B/Lee/40; lane 4, cells infected with B/Lee/40; lanes 5 to 9, the l[r] ...
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A Trio RhoA Shroom3 pathway is required for apical constriction and epithelial invagination
... and MDCK cells using Trizol (Invitrogen)/chloroform ...lens cells after they were flow-sorted for GFP. GFP+ lens cells were isolated from embryonic day (E) ...for MDCK cell ...
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Restriction of porcine parvovirus replication in nonpermissive cells.
... 66, 1992 RESTRICTION OF PPV REPLICATION IN NONPERMISSIVE CELLS nonstructural protein synthesis and progeny virus production in MDCK cells.. The deficiency in protein synthesis may be a d[r] ...
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Environmental Persistence of Influenza Viruses Is Dependent upon Virus Type and Host Origin
... HBE cells derived from human lung tissue were cultured at an air-liquid interface using an institutional review board-approved protocol ...HBE cells with phosphate-buffered saline (PBS) as previously ...
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Vaccinia virus preferentially enters polarized epithelial cells through the basolateral surface.
... To study the ability of VV to enter polarized cells through either the apical or basolateral surface, confluent and subconfluent monolayers of MDCK cells and Dll cells were infected with[r] ...
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Human Respiratory Syncytial Virus Glycoproteins Are Not Required for Apical Targeting and Release from Polarized Epithelial Cells
... polarized MDCK or non- polarized HEp-2 cells were plated on filter inserts and infected with engineered viruses at a multiplicity of ...the cells were fixed, permeabilized, and stained with anti-N, ...
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Influenza Virus M2 Ion Channel Protein Is Necessary for Filamentous Virion Formation
... in cells infected with the M2-Helix virus, filament formation was significantly impaired, suggesting that the combination of the five single alanine substitutions in this virus may be sufficient to disrupt either ...
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Most Influenza A Virions Fail To Express at Least One Essential Viral Protein
... h, cells were fixed, permeabilized, and then stained against the indicated viral ...(A) MDCK cells were infected with PR8 and then stained against HA (mouse MAb H36-26), NP (rabbit pAb), and M1/M2 ...
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Equine and Canine Influenza H3N8 Viruses Show Minimal Biological Differences Despite Phylogenetic Divergence
... in MDCK cells ...epithelial cells stained positive for both sialic acids, dog trachea epithelial cells showed stronger staining for ␣2-3-linked sialic ...
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MicroRNA-Based Attenuation of Influenza Virus across Susceptible Hosts
... unmodified MDCK cells, which lack miR-142, and MDCK cells engineered to express miR-142 ...MDCK142 cells, with a three- to four-log reduction in viral titer compared to that of ...
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