mean fluorescence intensity
Using the geometric mean fluorescence intensity index method to measure ZAP-70 expression in patients with chronic lymphocytic leukemia
... geometric mean fluorescence intensity (geo MFI) index method based on flow cytometry with 256-channel resolution in a series of 402 CLL patients and to compare ZAP-70 with other biological and ...
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Prognostic impact of ZAP 70 expression in chronic lymphocytic leukemia: mean fluorescence intensity T/B ratio versus percentage of positive cells
... Background: ZAP-70 is an independent negative prognostic marker in chronic lymphocytic leukemia (CLL). Usually, its expression is investigated by flow cytometric protocols in which the percentage of ZAP-70 positive CLL ...
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Chagasic patients are able to respond against a viral antigen from influenza virus
... percentage, mean fluorescence intensity (MFI) and integrated mean fluorescence intensity (iMFI) obtained multiplying the frequency by the ...
8
YC-1 induces lipid droplet formation in RAW 264.7 macrophages
... droplet formation in macrophages. We also demon- strated that the PKG inhibitor KT5823 inhibits YC-1- induced lipid droplet formation in RAW 264.7 macro- phages and foam cells, as evidenced by the reduction of the ...
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Cytokines Elicited by HSP60 in Periodontitis with and without Coronary Heart Disease
... the mean fluorescence intensity of intra- cellular TNF-α amongst groups with periodontal disease with/without coronary artery disease following stimula- tion with microbial HSP65 is consistent with ...
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Measles Virus Spread by Cell-Cell Contacts: Uncoupling of Contact-Mediated Receptor (CD46) Downregulation from Virus Uptake
... (mean fluorescence intensity [mfi]) was measured on the cell surface by flow cytometry on HeLa cells, which were mixed with uninfected U937 cells as a control for indicated times prior to removal of ...
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Effects of Biomimetic Shear Stress by Self-Assembled Gelatin Oleic Nanoparticles Containing Paclitaxel on the Behavior of Cancer Cell Lines in a Microfluidic System
... the fluorescence marker and model drug, respectively, and were loaded into GONs by incubation (C-GONs; ...of fluorescence labeled C-GONs and the drug efficacy of ...the mean fluorescence ...
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Association between autophagy and inflammation in patients with rheumatoid arthritis receiving biologic therapy
... Fig. 2 Representative cytometric histograms of p62 levels in lymphocytes (a1 and b1), monocytes (a2 and b2), and granulocytes (a3 and b3) from one patient with rheumatoid arthritis (RA) and one healthy control subject ...
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Altered Monocyte Phenotype in HIV-1 Infection Tends to Normalize with Integrase-Inhibitor-Based Antiretroviral Therapy
... Results In untreated HIV-1 infection there were perturbations in monocyte subset phenotypes, chiefly a higher frequency and density mean fluorescence intensity–MFI of HLA-DR %-p = 0.004,[r] ...
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The GIP receptor displays higher basal activity than the GLP-1 receptor but does not recruit GRK2 or arrestin3 effectively.
... Using a luciferase-based reporter gene assay, we detected significantly higher constitutive activity in GIPR compared with GLP-1R (Figure 1A and B). Increased levels of receptor expression can amplify basal activity ...
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FcgammaRIIIa: Tissue distribution and regulation of expression
... Figure 3 .l i d The mean fluorescence intensity of FcyRHIa on monocytes 210 cultured with normal growth medium, conditioned medium from non-stretched synovial fibroblasts and condi[r] ...
286
Original Article Inhibitory effect of protectin D1 on NALP3 inflammasomes
... the mean fluorescence intensity ROS in hepatocytes was measured using the flow cytometry, whereas qRT-PCR and Western blot were applied to measure the mRNA and protein levels of NALP3, ASC and ...
7
Toll like receptor 3 upregulation in macrophages participates in the initiation and maintenance of pristane induced arthritis in rats
... The mean fluorescence intensity MFI of cell surface TLR3 positive cell increased markedly at d6 and the proportion increased significantly at d26, although the intracellular TLR3 express[r] ...
12
An association of Aquaporin 4 with the immunoregulation of liver pathology in mice infected with Schistosoma japonicum
... Studies have shown that CD4 + CD25 + Foxp3 + Treg cells are induced mainly by egg antigens during the infec- tion, and play an important suppressive role in down- modulating granulomatous response in schistosomiasis ...
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Study of Myeloid Antigens CD13 and CD33 Expression in Sudanese Patients with Acute Leukemia
... Depending up on pilot study in the quality control results (that saved in the Q.C system II software file) of EPICS XL Flowcytometer, which adjusted the cut off points between negative and positive scale for every ...
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Assessing the Efficacy of Nano- and Micro-Sized Magnetic Particles as Contrast Agents for MRI Cell Tracking
... the mean green fluorescence of individual ...the mean fluorescence intensity as a function of contrast agent concentration was exactly the same as observed quantitatively by ...
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The importance of claudin-7 palmitoylation on membrane subdomain localization and metastasis-promoting activities
... and mean ± SD (triplicates) of wound closure are shown (scale bar: 250 μ ...lysis; mean ± SD of ...the mean ± SD migration of 20 individual ...
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The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin
... IR, fluorescence spectroscopy has the advantages of fast speed and low cost of ...the fluorescence spectra of ENR under different pH conditions were investigated to determine the structural ...
6
Established cell surface markers efficiently isolate highly overlapping populations of skeletal muscle satellite cells by fluorescence-activated cell sorting
... fluorescent intensity to arrive at the chosen dilutions of 1:200 for α7-integrin, 1:100 for β1-integrin, CXCR4, and VCam1, and 1:25 for CD34 (Additional file 1: Figure ...
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Autonomous buckling of micrometer-sized lipid-protein membrane patches constructed by Dictyostelium discoideum
... The cytosol of Dictyostelium discoideum contains various types of membrane-binding proteins and/or lipid-conversion enzymes such as phospholipases, lipases, and phosphokinases. Since PH-crac and PTEN play important roles ...
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