membrane-proximal external region
Broad Neutralization of Human Immunodeficiency Virus Type 1 Mediated by Plasma Antibodies against the gp41 Membrane Proximal External Region
... anti-membrane proximal external region (MPER) peptide binding antibodies from among 156 chronically human immunodeficiency virus type 1-infected ...
10
Generation of Long-Lived Bone Marrow Plasma Cells Secreting Antibodies Specific for the HIV-1 gp41 Membrane-Proximal External Region in the Absence of Polyreactivity
... gp41 membrane-proximal external region (MPER) amino acid sequences across HIV-1 clades and the ability of anti-MPER broadly neutralizing antibodies (BNAbs) to block viral hemifusion/fusion ...
16
4E10-Resistant Variants in a Human Immunodeficiency Virus Type 1 Subtype C-Infected Individual with an Anti-Membrane-Proximal External Region-Neutralizing Antibody Response
... The membrane-proximal external region (MPER) of the human immunodeficiency virus type 1 (HIV-1) envelope gly- coprotein comprises the last 23 amino acids, from residues 660 to 683, of the ...
9
Reversible Inhibition of Fusion Activity of a Paramyxovirus Fusion Protein by an Engineered Disulfide Bond in the Membrane-Proximal External Region
... a region of F that forms a 3-helix bundle (3-HB) stalk domain and in the mono- mer is known as HRB, and a prehairpin intermediate in which the fusion peptide (FP) and heptad repeat A (HRA) are released and the FP ...
5
Antigenic and Immunogenic Study of Membrane-Proximal External Region-Grafted gp120 Antigens by a DNA Prime-Protein Boost Immunization Strategy
... The membrane-proximal external region (MPER) of human immunodeficiency virus type 1 (HIV-1) gp41 is a target of broadly neutralizing monoclonal antibodies (MAbs) 2F5, 4E10, and ...V1/2 ...
14
Cholesterol-Dependent Membrane Fusion Induced by the gp41 Membrane-Proximal External Region–Transmembrane Domain Connection Suggests a Mechanism for Broad HIV-1 Neutralization
... viral membrane with that of the target ...its membrane-proximal external region (MPER) may interact with the HIV-1 membrane and in- duce its disruption and/or deformation during ...
11
Broadly Neutralizing Monoclonal Antibodies 2F5 and 4E10 Directed against the Human Immunodeficiency Virus Type 1 gp41 Membrane-Proximal External Region Protect against Mucosal Challenge by Simian-Human Immunodeficiency Virus SHIVBa-L
... The membrane-proximal external region (MPER) of HIV-1, located at the C terminus of the gp41 ectodo- main, is conserved and crucial for viral ...conserved region an important potential ...
12
Antibodies Generated in Cats by a Lipopeptide Reproducing the Membrane-Proximal External Region of the Feline Immunodeficiency Virus Transmembrane Enhance Virus Infectivity
... C-terminal region of the TM of FIV and location of the synthetic peptides used in the ...-helix region; MPER, membrane-proximal external region; TrpM, Trp-rich motif; MSR, ...
8
Direct Antibody Access to the HIV-1 Membrane-Proximal External Region Positively Correlates with Neutralization Sensitivity
... Neutralizing antibodies can either efficiently and directly recognize the prereceptor-engaged native spike on the virus surface or require receptor engagement to better expose spe- cific neutralizing determinants. ...
10
Stable Docking of Neutralizing Human Immunodeficiency Virus Type 1 gp41 Membrane-Proximal External Region Monoclonal Antibodies 2F5 and 4E10 Is Dependent on the Membrane Immersion Depth of Their Epitope Regions
... viral membrane and gp41 membrane proximal external region (MPER) ...interfacial region of the lipid ...the membrane via a hydrophobic anchor sequence was required for ...
13
Rational Design of Membrane Proximal External Region Lipopeptides Containing Chemical Modifications for HIV-1 Vaccination
... the membrane proximal external region (MPER) of HIV-1 gp41 using current vaccine strategies has hampered efforts to prevent the spread of ...
7
Engineering Recombinant Reoviruses To Display gp41 Membrane-Proximal External-Region Epitopes from HIV-1
... and membrane-proximal external region (MPER) ...transmembrane region also is ...crystallized region are shown in red, blue, and yellow; the model is shown in ...
15
Neutralization Efficiency Is Greatly Enhanced by Bivalent Binding of an Antibody to Epitopes in the V4 Region and the Membrane-Proximal External Region within One Trimer of Human Immunodeficiency Virus Type 1 Glycoproteins
... The envelope glycoproteins (Envs) of human immunodefi- ciency virus type 1 (HIV-1) exist on the virion or cell surface as trimers of gp120 and gp41 heterodimers (13, 30, 62, 65). High- resolution structural information ...
10
Chemically Modified Peptides Based on the Membrane-Proximal External Region of the HIV-1 Envelope Induce High-Titer, Epitope-Specific Nonneutralizing Antibodies in Rabbits
... The membrane proximal external region (MPER) of the gp41 transmembrane protein in the HIV-1 envelope (1) is an obvious target for vaccine development due to the conserved sequence and ...
8
Neutralizing Epitopes in the Membrane-Proximal External Region of HIV-1 gp41 Are Influenced by the Transmembrane Domain and the Plasma Membrane
... viral membrane but have poor neutralizing (Nt) efficiency across diverse HIV-1 strains (47, ...The membrane-proximal external region of HIV-1 gp41 (MPER) is one of the most highly ...
12
Utilization of Immunoglobulin G Fc Receptors by Human Immunodeficiency Virus Type 1: a Specific Role for Antibodies against the Membrane-Proximal External Region of gp41
... constant region of immunoglobulin G (IgG) are an important link between humoral immunity and cellular ...the membrane-proximal external region (MPER) of gp41 were potentiated by Fc ␥ RI ...
14
Broadly Neutralizing Antibodies Targeted to the Membrane-Proximal External Region of Human Immunodeficiency Virus Type 1 Glycoprotein gp41
... the region to which Z13 and 4E10 bind is an immunodominant site on gp41 ...this region must, however, be put into context since previous serum panel screens with overlapping peptides failed to iden- tify ...
14
HIV-1 Envelope Glycoprotein Trimers Display Open Quaternary Conformation When Bound to the gp41 Membrane-Proximal External-Region-Directed Broadly Neutralizing Antibody Z13e1
... We prepared frozen-hydrated specimens of soluble gp140 trimers incubated with Z13e1 Fab fragments and recorded projec- tion images in a Titan Krios electron microscope equipped for operation at liquid nitrogen ...
6
Anti-Human Immunodeficiency Virus Type 1 (HIV-1) Antibodies 2F5 and 4E10 Require Surprisingly Few Crucial Residues in the Membrane-Proximal External Region of Glycoprotein gp41 To Neutralize HIV-1
... A recent report suggested that serum antibodies against T20 do not reduce its potency in vivo (67). Such antibodies may be more like D50 rather than 2F5, the latter of which we showed here to inhibit the activity of T20. ...
10
Importance of the Membrane-Perturbing Properties of the Membrane-Proximal External Region of Human Immunodeficiency Virus Type 1 gp41 to Viral Fusion
... viral membrane like a ...plasma membrane as widely spaced parts of a single ...close membrane association of the MPER region in its an- tibody-sensitive conformation ...
9