MEMBRANE TOPOLOGY
Dissecting the Gene Expression, Localization, Membrane Topology, and Function of the Plasmodium falciparum STEVOR Protein Family
... plasma membrane and apical organelles, pointing toward a puta- tive function in host cell egress or ...cell membrane, and tracked them to a secondary location, either to the parasite plasma membrane ...
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Studies on membrane topology, N glycosylation and functionality of SARS CoV membrane protein
... Influence of M on the intracellular distribution of S Surface spikes composed of the SARS-CoV glycoprotein S confer the name-giving shape of coronaviral particles in electron microscopy analysis [35]. The spikes coat the ...
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Bitopic Membrane Topology of the Stable Signal Peptide in the Tripartite Junín Virus GP-C Envelope Glycoprotein Complex
... SSP topology in the Old World ...the membrane topology of the SSP of the Old World LCMV and Lassa fever virus have yielded different and mutually conflicting results (18, ...bitopic topology ...
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Membrane Topology Mapping of the O Antigen Flippase (Wzx), Polymerase (Wzy), and Ligase (WaaL) from Pseudomonas aeruginosa PAO1 Reveals Novel Domain Architectures
... inner membrane proteins Wzx (O-antigen [O-Ag] flippase), Wzy (O-Ag polymerase), and WaaL (O-Ag ...the membrane topology of these ...trans- membrane (TM), or periplasmic ...inner ...
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Plasma Membrane Topology of Syncytial Domains of Herpes Simplex Virus Type 1 Glycoprotein K (gK): the UL20 Protein Enables Cell Surface Localization of gK but Not gK-Mediated Cell-to-Cell Fusion
... cellular membrane fusion has been controversial, largely due to previously unsuccessful efforts to detect gK expression on virion and cellular ...its membrane topology, we generated the recombinant ...
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Localization and Membrane Topology of Coronavirus Nonstructural Protein 4: Involvement of the Early Secretory Pathway in Replication
... nsp4-EGFP, or the N-terminally tagged MHV nsp4-EGFP gene, respectively, under the control of a cytomegalovirus pro- moter, were infected with MHV-A59 at 24 h posttransfection. At 6 h postinfection, the cells were fixed ...
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Glycoprotein 3 of Porcine Reproductive and Respiratory Syndrome Virus Exhibits an Unusual Hairpin-Like Membrane Topology
... secretion, membrane anchoring, and the membrane topology of GP3 from PRRSV-1 and PRRSV-2 ...hairpin-like membrane topology ...I membrane protein containing a transmembrane region ...
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Membrane Topology and Function of Dengue Virus NS2A Protein
... of membrane topology and analysis of membrane-associ- ated activity of DENV-2 ...(D) Membrane flotation analysis of 293T cells transfected with plasmids expressing NS2A fragment- eGFP fusion ...
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A Yeast Golgi E-type ATPase with an Unusual Membrane Topology
... vacuolar membrane, and plasma membrane are found in the 13,000 3 g pellet (40, 41), whereas the 120,000 3 g pellet (P120) is a Golgi-enriched fraction, containing the majority of the Golgi enzymes a ...
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Experimental determination of the membrane topology of the Plasmodium Protease Plasmepsin V
... the topology of Plas- mepsin V in the endoplasmic reticulum membrane; we show that the catalytic protease do- main of Plasmepsin V faces the endoplasmic reticulum ...
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Disulfide Bonds and Membrane Topology of the Vaccinia Virus A17L Envelope Protein
... two membrane-spanning domains and placed cys- teines 101 and 121 in an intraluminal location (1), consistent with stoichiometric disulfide bond ...four membrane-spanning domains (11) would place cysteines ...
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Evidence for Mixed Membrane Topology of the Newcastle Disease Virus Fusion Protein
... Inhibition of fusion by antibody to CT sequences. To ask if the CT sequences detected at cell surfaces played any role in cell-cell fusion, the effect of antibody to CT sequences on membrane fusion was determined. ...
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Processing and membrane topology of the spike proteins G1 and G2 of Uukuniemi virus.
... The membrane glycoproteins G1 and G2 of the members of the Bunyaviridae family are synthesized as a precursor from a single open reading ...and membrane insertion of G1 and G2 of a member of the Phlebovirus ...
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Location and unusual membrane topology of the immunity protein of the Escherichia coli phage T4.
... Specific localization of the lysis protein of bacteriophage MS2 in membrane adhesion sites of Escherichia coli.. In vivo synthesis and properties of uracil-containing DNA.[r] ...
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Membrane Topology and Transient Acylation of Toxoplasma gondii Glycosylphosphatidylinositols
... GlcN-PI), were located on the outer (cytoplasmic) side of the microsomal membranes (52). Here we report the topological localization of early and late GPI anchor intermediates in the pathogen T. gondii, including for the ...
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The effect of membrane topology features on ion transport phenomena
... A membrane which is slightly too large will make it more difficult to tighten the cell parts and has to be compressed resulting in bending of the ...the membrane is too small it will not cover the channel ...
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Membrane topology analysis of HIV 1 envelope glycoprotein gp41
... Although the bacterial system is quick and informative, eukaryote specific post-translational modifications and/ or the difference in the composition of lipids in the membrane may affect the topology of ...
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Transmembrane Topology of PiT-2, a Phosphate Transporter-Retrovirus Receptor
... current membrane topology model of PiT-1 and PiT-2 predicts 10 transmembrane ...PiT-2 topology, may be useful for further investigations of PiT-2 interactions with other proteins and for the ...
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Unusual Topological Arrangement of Structural Motifs in the Baboon Reovirus Fusion-Associated Small Transmembrane Protein
... plasma membrane since p15-HAC retained the full cell-cell fusion activity of authentic p15 (data not shown) implying plasma membrane localization in the cor- rect ...bitopic membrane topology ...
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Topology of the large envelope protein of duck hepatitis B virus suggests a mechanism for membrane translocation during particle morphogenesis.
... the membrane are ...the topology of the large envelope protein of DHBV in extracellular virus ...the topology of L protein in virions because, for example, DHBsAg and DHBV both bind to susceptible ...
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