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Mesenchymal Stem Cell Culture

XanoMatrix surfaces as scaffolds for mesenchymal stem cell culture and growth

XanoMatrix surfaces as scaffolds for mesenchymal stem cell culture and growth

... for mesenchymal stem cell culture and growth has been widely used as a prosthetic vascular graft and has shown excellent mechanical strength and good ...natural stem cell ...

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Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering

Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering

... human mesenchymal stem cell culture in tissue engineering Abstract The design of new bioactive scaffolds mimicking the physiologic environment present during tissue formation is an important ...

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The role of dissolved oxygen levels on human Mesenchymal Stem Cell culture success, regulatory compliance and therapeutic potential

The role of dissolved oxygen levels on human Mesenchymal Stem Cell culture success, regulatory compliance and therapeutic potential

... a cell culture system is complicated because O 2 exists in two phases: gaseous in the headspace and dissolved in the liquid ...tissue culture workstation set at 5% O 2 level, a liquid medium can hold ...

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Mesenchymal stem cell fate : applying biomaterials for control of stem cell behavior

Mesenchymal stem cell fate : applying biomaterials for control of stem cell behavior

... desired cell response and could also be biodegradable with the aim of being replaced with native tissue after the support and templating role was complete; second-generation biomaterials included hydroxyapatite ...

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Mechanisms of mesenchymal stem/stromal cell function

Mechanisms of mesenchymal stem/stromal cell function

... The role of MSCs in the protection of other damaged tissues has also been demonstrated. For example, intra- peritoneally and intravenously administered MSCs from murine bone marrow and adipose tissue had a protective ...

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The Effect of Hypoxia on Mesenchymal Stem Cell
Biology

The Effect of Hypoxia on Mesenchymal Stem Cell Biology

... and culture in normoxia) and MSC-PBH (MSCs that collect from peripheral blood and culture in hypoxia) after two passages in culture is matched with BM ...(homing-associate cell adhesion ...

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Keywords: mesenchymal cell osteoarthritis, stem cell, umbilical cord

Keywords: mesenchymal cell osteoarthritis, stem cell, umbilical cord

... cord-mesenchymal stem cells differentiation assay [11] Chondrogenic differentiation For chondrogenic differentiation, the pellet culture system described by Sekiya et ...

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A review of therapeutic effects of mesenchymal stem cell secretions and induction of secretory modification by different culture methods

A review of therapeutic effects of mesenchymal stem cell secretions and induction of secretory modification by different culture methods

... proliferating cell nuclear antigen-positive hepatocytes, liver weight/body weight ratio, and survival compared with animals that received normoxia preconditioned ...

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Effects of Mesenchymal Stem
Cell Derivatives on Hematopoiesis and Hematopoietic Stem Cells

Effects of Mesenchymal Stem Cell Derivatives on Hematopoiesis and Hematopoietic Stem Cells

... G-CSF, stem cell factor (SCF), leukemia inhibitory factor (LIF), macrophage- CSF (M-CSF), IL-6, and IL-11 within the in vitro culture medium with a role in the adjustment of the differentiation of ...

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Characterization of Embryonic Stem Cell-Differentiated Cells as Mesenchymal Stem Cells

Characterization of Embryonic Stem Cell-Differentiated Cells as Mesenchymal Stem Cells

... Type Culture Collection (ATCC) (Ryan. 2008). Most cell lines derived from normal tissues contain cells that are anchorage- ...a cell culture ...coat cell culture dishes and allow ...

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Role of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Epithelial-Mesenchymal Transition

Role of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Epithelial-Mesenchymal Transition

... epithelial/mesenchymal cell markers, the morphology and growth pattern of breast cancer cells, the increased expression of genes associated with invasion and migration, angiogenesis, and anti-apoptosis ...

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Cell number in mesenchymal stem cell aggregates dictates cell stiffness and chondrogenesis

Cell number in mesenchymal stem cell aggregates dictates cell stiffness and chondrogenesis

... vitro culture or in vivo implantation is costly, time-consuming, and re- mains the rate-limiting step for clinical translation due to the inverse correlation between MSCs differentiation potential and expansion ...

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Phenotypic traits of mesenchymal stem cell sheets fabricated by temperature-responsive cell culture plate: structural characteristics of MSC sheets

Phenotypic traits of mesenchymal stem cell sheets fabricated by temperature-responsive cell culture plate: structural characteristics of MSC sheets

... most stem cell therapy strategies reported to date, stem cells are introduced to damaged tissue sites to repair and regenerate the original tissue structure and ...including cell adhesion and ...

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Stem cell treatment for patients with autoimmune disease by systemic infusion of culture expanded autologous adipose tissue derived mesenchymal stem cells

Stem cell treatment for patients with autoimmune disease by systemic infusion of culture expanded autologous adipose tissue derived mesenchymal stem cells

... diseases. Stem cells have become a promising tool for their treatment by promoting tissue repair and protection from immune-attack associated ...autologous stem cells present a safe option for this ...

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Applications of mesenchymal stem cell technology in bovine species

Applications of mesenchymal stem cell technology in bovine species

... rapid cell culture and expansion that can take place after enzymatic disaggregation of ...into cell types from all three germ layers, a typical minimum criterion for a cell type to be ...

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Mesenchymal Stem Cell functionalization for enhanced therapeutic applications

Mesenchymal Stem Cell functionalization for enhanced therapeutic applications

... including cell selection and priming ...MSC culture-expansion methods, although proven to be clinically safe, do not guarantee the preservation of specific native MSC characteristics and attributes, thus ...

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Potential of Mesenchymal Stem Cell based application in Cancer.

Potential of Mesenchymal Stem Cell based application in Cancer.

... Stem cell based treatments are being increasingly explored for their possible potential to treat various ...cancers. Mesenchymal stem cells believed to possess anti-tumor potential and are ...

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Mesenchymal Stem Cell-Dependent Modulation of Liver Diseases

Mesenchymal Stem Cell-Dependent Modulation of Liver Diseases

... of culture-expanded MSCs, but also the mechanism of interactions between MSCs and immune cells that may be of relevance to future widespread clinical use of MSCs in therapy of liver ...

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Human Mesenchymal Stem Cell Functional Identification Kit

Human Mesenchymal Stem Cell Functional Identification Kit

... Adipogenesis Culture Protocol. ADIPOGENESIS CULTURE PROTOCOL Note: 50 mL of Adipogenic Differentiation Media is sufficient to culture and differentiate 16 wells for 21 days with 6 media ...

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Defining human mesenchymal stem cell efficacy in vivo

Defining human mesenchymal stem cell efficacy in vivo

... increased pulmonary inflammation and histology consis- tent with increased mucus production and eosinophils. hMSC treatment of acute asthma mice resulted in decreased airway inflammation as determined by BAL. ...

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