Metallo-β-lactamase
Structural and biochemical characterization of Rm3, a subclass B3 Metallo β Lactamase identified from a functional metagenomic study
... Characterization of Rm3 Metallo-β-Lactamase active site, the Rm3 structure more closely resembles that of the mobile B3 enzymes AIM-1 and.. In contrast to these clear differences in over[r] ...
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Detection Rate of Metallo-β-Lactamase-Expressing Genes; blaVIM-1, blaVIM-2 and blaSPM-1 in Pseudomonas aeruginosa Isolates
... Introduction: Imipenem-resistant Pseudomonas aeruginosa is an organism expressing metallo- β-lactamase (MBL) enzyme, and is a serious agent of hospital infection holding a serious universal ...
5
Distribution of Integrons and Gene Cassettes among Metallo-β-Lactamase Producing Pseudomonas aeruginosa Clinical Isolates
... The emergence of multidrug resistant (MDR) Pseudomonas aeruginosa has become a serious problem for healthcare settings in developing countries (1). The dissemination of antibiotic resistance genes by horizontal transfer ...
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Prevalence of metallo β lactamase acquired genes among carbapenems susceptible and resistant Gram negative clinical isolates using multiplex PCR, Khartoum hospitals, Khartoum Sudan
... The most prevalent families of MBL resistant genes worldwide are IMP (inactivate imipenem, first reported in Pseudomonas aeruginosa strain from Japan in 1988) [10]. While the VIM (Verona Integron-encoded Metallo- ...
6
Increasing Carbapenem Resistant Gram Negative Bacilli and Decreasing Metallo β Lactamase Producers over Eight Years from Korea
... The trends and types of carbapenemase-producing Gram-negative bacilli were an- alyzed from clinical specimens collected between 2005 and 2012 at a Korean teaching hospital. The proportions of carbapenem-resistant ...
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Thiol-Containing Metallo-β-Lactamase Inhibitors Resensitize Resistant Gram-Negative Bacteria to Meropenem
... by metallo- β -lactamase (MBL) expressing Gram-negative bacteria has grown at an alarming rate in recent ...all β -lactam antibiotics, there are as of yet no approved drugs available that ...
7
Emergence of New Delhi Metallo-β-Lactamase (NDM) Producing Multidrug-Resistant Escherichia Coli in Sudan
... he was hospitalized, initially in the Punjab area, but then in New Delhi, for the management of a gluteal abscess. In January 2008 he was admitted to a hospital in Orebro, Sweden, where, on the day after admission, a ...
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NDM 1 Metallo β Lactamase and ArmA 16S rRNA methylase producing Providencia rettgeri clinical isolates in Nepal
... harboring metallo- β -lactamase (MBL) encoding genes, including IMP-type MBL producers in Japan [3,4]; VIM-type MBL, PER-1 extended-spectrum β - lactamase (ESBL) and 16S rRNA methylase ...
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Effect of metallo β lactamase production and multidrug resistance on clinical outcomes in patients with Pseudomonas aeruginosabloodstream infection: a retrospective cohort study
... of metallo-β- lactamase (MBLs) which confer resistance to all β-lactams except aztreonam is a mechanism of increasing clinical importance, largely driven by the international spread of ...
9
Nosocomial Outbreak Caused by Multidrug Resistant Pseudomonas aeruginosa Producing IMP 13 Metallo β Lactamase
... Detection of  -lactamase activity. Phenotypic screening for MBL production was carried out by the Etest MBL assay (AB Biodisk, Solna, Sweden), under the conditions recommended by the test manufacturer, and by the ...
5
Detection of Metallo β Lactamase in lactose non fermenting gram negative organisms comparison of different methods
... of β- lactamases and Carbapenemases in Enterobacteriaceae, it has not yet advocated any standard test for detection of MBLs and Carbapenemases in lactose non fermenting Gram negative ...
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EDTA Modified Carbapenem Inactivation Method: a Phenotypic Method for Detecting Metallo β Lactamase Producing Enterobacteriaceae
... O ne of the most concerning forms of antimicrobial resistance in Gram-negative bacteria is resistance to the carbapenems, potent broad-spectrum  -lactam agents. Due to the ability of Enterobacteriaceae to readily spread ...
9
Molecular Evolution of Metallo β Lactamase Producing Pseudomonas aeruginosa in a Nosocomial Setting of High Level Endemicity
... Pseudomonas aeruginosa remains one of the most important pathogens in the nosocomial setting, where it is a common causative agent of bacteremia, pneumonia, and urinary tract infections (10). Acquired drug resistance is ...
6
The determination of the crystal structure of the metallo β-lactamase from Stenotrophomonas maltophilia and mechanistic studies.
... The fact that the class B P-lactamases contain zinc ions provides a convenient way of determining phases by the MAD method. However, it would only be possible with enzymes containing binuclear zinc centres as a single ...
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In silico fragment based design identifies subfamily B1 metallo-β-lactamase inhibitors
... -Lactam-containing molecules remain the single most important antibacterials. 1-2 -Lactams inhibit transpeptidases (or penicillin-binding proteins, PBPs) involved in cell wall biosynthesis by reacting with a ...
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Phenotypic Detection of Carbapenem Susceptible Metallo β Lactamase Producing Gram Negative Bacilli in the Clinical Laboratory
... The clinical utility of carbapenems is under threat with the emergence of acquired carbapenemases, particularly Ambler class B metallo-  -lactamases (MBLs). Such enzymes have emerged in many geographical ...
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Infections with VIM 1 Metallo β Lactamase Producing Enterobacter cloacae and Their Correlation with Clinical Outcome
... The preliminary antibiotic resistance profile determined by using the Vitek 2 automatic system was confirmed by the broth microdilution method, using Muel- ler-Hinton broth, according to CLSI guidelines. All experiments ...
6
Detection of Metallo β lactamase in gram negative bacilli
... Three different phenotypic methods were used to confirm the MBL production: Ertapenem ETP-EDTA ETP EDTA combined disc test, EDTA disc potentiation using Ceftazidime and Modified Hodge Te[r] ...
5
Structural basis of metallo-β-lactamase, serine-β-lactamase and penicillin binding protein inhibition by cyclic boronates
... and metallo- hydrolases is also of interest in other drug discovery fields, including cancer, where it may be desirable to inhibit different classes of functionally related proteases 27 ...
8
IMP-68, a Novel IMP-Type Metallo-β-Lactamase in Imipenem-Susceptible Klebsiella pneumoniae
... MP-type metallo-  -lactamases are among the most common families of acquired carbapenemases detected from Enterobacteriaceae and have been reported mainly in East Asia, including Japan ...IMP-type metallo- ...
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