Methane monooxygenase
Mutagenesis and expression of methane monooxygenase to alter regioselectivity with aromatic substrates
... Soluble methane monooxygenase (sMMO) from methane-oxidising bacteria can oxygenate more than 100 hydrocarbons and is one of the most catalytically versatile biological oxidation ...
7
Mutagenesis of the "Leucine Gate" to explore the basis of catalytic versatility in soluble methane monooxygenase
... Soluble methane monooxygenase (sMMO) from methane-oxidizing bacteria is a multicomponent non-heme oxygenase that naturally oxidizes methane to methanol and can also co-oxidize a wide range of ...
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The particulate methane monooxygenase from Methylococcus capsulatus (Bath)
... Protein B of soluble methane monooxygenase from Methylococcus capsulatus Bath: a novel regulatory protein of enzyme activity.. The biosynthesis and assembly of protein A of soluble metha[r] ...
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The soluble methane monooxygenase and ammonia oxidation in the obligate methanotroph "Methylosinus trichosporium (OB3b)"
... Properties of the methane monooxygenase from extracts of Methylosinus trichosporium OB3b and evidence for i t ' s similarity to the enzyme from Methylococcus capsulatus Bath... oxidation[r] ...
260
Development of genetic tools in methanotrophs and the molecular regulation of methane monooxygenase
... Regulation of bacterial methane oxidation: Transcription of the soluble methane monooxygenase operon of Methylococcus capsulatus Bath is repressed by copper ions.. Copper-dependent recip[r] ...
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Survey of methanotrophic diversity in various ecosystems by degenerate methane monooxygenase gene primers
... late methane monooxygenase (pMMO) catalyzes the hydroxylation of ...ammonia monooxygenase super- family and has been shown to be of high biogeochemi- cal and chemical relevance (Bédard and Knowles ...
11
Methanobactin and the membrane associated methane monooxygenase in methanotrophy: a tale of two proteins
... Chapter 6 presents the potential secondary roles of Cu-mb in methane catalysis by the pMMO. The oxidase, superoxide dismutase, and hydrogen peroxide reductase activites of mb were examined and compared to the ...
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The regulatory protein and component interactions of soluble methane monooxygenase
... The scattering results are consistent with prior evidence for the physical interactions within the sMMO complex, including titrations of the hydroxylase with reductase and protein B, whi[r] ...
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Mutagenic studies into the catalytic versatility of soluble methane monooxygenase
... monooxygenase of Burkholderia cepacia G4 were created by Wood and co-workers (Canada et al. 2002) using DNA shuffling. A number of tom genes were digested with DNase I and randomly reassembled to create a mutant ...
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Heterologous expression and site directed mutagenesis of soluble methane monoxygenase
... The purpose of this investigation was to study the heterologous expression of soluble methane monooxygenase sMMO genes from Methylococcus capsula/us Bath and Methylosinus trichosporium O[r] ...
355
Activity-Based Protein Profiling of Alkane-Oxidizing Monooxygenases in Rhodococcus rhodochrous ATCC 21198.
... In this study, the growth gaseous alkane growth substrate range of R. rhodochrous ATCC 21198 has been characterized and it has been demonstrated that cells grown on gaseous alkanes can cometabolically oxidize the ...
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Gene regulation in methanotrophs : evidence from Methylococcus capsulatus (Bath) and Methylosinus trichosporium (OB3b)
... particulate methane monooxygenase enzyme. However, a few methanotrophic species contain only soluble methane monooxygenase enzyme e.g., M. silvest ris (Dunfield et al., 2003; Theisen et al., ...
285
Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1
... utilize methane (the majority component of natural gas and biogas) as their sole carbon and energy source [5, ...utilizing methane oxidizing bacteria that does not have the high capital and operations ...
8
Cavity Ring-Down and Multi-Pass Spectroscopies for Methane Source Attribution and Chemical Kinetics Studies
... Saunois, M., Bousquet, P., Poulter, B., Peregon, A., Ciais, P., Canadell, J.G., Dlugokencky, E.J., Etiope, G., Bastviken, D., Houweling, S., Janssens-Maenhout, G., Tubiello, F.N., Castaldi, S., Jackson, R.B., Alexe, M., ...
189
Reactor Modeling of a Non-Catalytic OCM Process
... OCM is one of the direct processes for methane conversion to more valuable pro- ducts, and has been the subject of widespread research in recent years. This process is usually performed catalytically; however, ...
12
Biotechnological conversion of methane to methanol: evaluation of progress and potential
... Methanotroph species are now known in Proteobacteria, Verrucomicrobia and candidate phylum NC10. Proteobacteria methanotrophs are divided into two classes; Alphaproteobacteria and Gammaproteobacteria. Current ...
39
A fast and sensitive activity assay for lytic polysaccharide monooxygenase
... The suggested standard conditions for the LPMO activ- ity assay are a temperature of 30 °C, an H 2 O 2 concentra- tion of 100 µM, a 2,6-DMP concentration of 1.0 mM, a 100 mM sodium s[r] ...
13
Regioselectivity of oxidation by a polysaccharide monooxygenase from Chaetomium thermophilum
... To confirm the involvement of the residues Tyr27, His64, His157 and Tyr206 of CtPMO1 in C4 and C6 oxi- dation, soluble reaction products of the mutated enzymes were oxidized by Br 2 [r] ...
16
Mechanistic aspects of the monooxygenase system from Methylococcus capsulatus
... m u l t i p l e t a t 2.2 p.p.m. was a b s e n t. As the H NMR spectrum shows th e r e i s no peak around 2.3 p.p.m . , i t can be con clu ded th at o x i d a t i o n has oc c urre d in a c i / u - s t e r e o s p e c i ...
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Downstream Processing from Baeyer-Villiger Monooxygenase Bioconversions
... The application o f batch liquid-liquid extraction is poor due to the low partition coefficient o f the products. This system is designed for use with low partiti[r] ...
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