methylation-specific PCR
Direct Bisulfite Sequencing and Methylation Specific PCR to Detect Methylation of p15INK4b and F7 genes in Coronary Artery Disease Patients
... promoter methylation through epigenetic modifications at the promoter site may regulate transcription of F7 gene ...DNA methylation) is defined as stable and heritable changes that are not due to disrupting ...
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MGMT promoter methylation in gliomas assessment by pyrosequencing and quantitative methylation specific PCR
... DNA methylation induced by TMZ is located at the O 6 -position of guanine and methylation in this position is considered to be the main contributor to the cytotoxic effect ...hyper- methylation was ...
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Detection of nasopharyngeal carcinoma in Morocco (North Africa) using a multiplex methylation-specific PCR biomarker assay
... (DNA) methylation of cyclin-dependent kinase inhibitor 2A (P16) and RASSF1A loci have been reported to occur in premalignant nasopha- ryngeal epithelium even prior to EBV infection ...rant methylation of ...
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Evaluating the Role of PTEN Promoter Methylation in Patients Predisposed to Hypercoagulable States via Methylation Specific PCR
... 20. Datta J, Ghoshal K, Denny WA, Gamage SA, Brooke DG, Phiasivongsa P, et al. A new class of quinoline-based DNA hypomethylating agents reactivates tumor suppressor genes by blocking DNA methyltransferase 1 activity and ...
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Two Steps Methylation Specific PCR for Assessment of APC Promoter Methylation in Gastric Adenocarcinoma
... Promoter methylation could be observed in many types of cancers leading to aberrant transcriptional silencing of tumor suppressor genes [29]. Our results showed that 28 (77.77%) out of 36 patients are methylated. ...
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miR-506 attenuates methylation of lncRNA MEG3 to inhibit migration and invasion of breast cancer cell lines via targeting SP1 and SP3
... MEG3 methylation level and increased MEG3 ...out methylation-specific PCR to examine MEG3 methyl- ation level and observed that, in both MCF-7 and MDA- MB-231 cells, SP3 knockdown ...
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Author Index
... Direct Bisulfite Sequencing and Methylation Specific PCR to Detect Methylation of p15INK4b and F7 genes in Coronary Artery Disease Patients.. 1 23-29.[r] ...
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Cell-free DNA promoter hypermethylation in plasma as a diagnostic marker for pancreatic adenocarcinoma
... clinical relevant control groups. We designed the gene- panel primary based on our literature review addressing genes aberrantly methylated in pancreatic adenocarcin- oma [16]. This approach was used, to evaluate the ...
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Promoter methylation of Wnt-antagonists in polypoid and nonpolypoid colorectal adenomas.
... Promoter methylation of these four genes was determined using quantitative methylation- specific PCR (qMSP) [25] in a well-characterized series of both nonpolypoid and polypoid adenomas, and ...
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Promoter methylation regulates cyclooxygenase expression in breast cancer
... DNA methylation is an epigenetic modification that can play an important role in silencing of other genes ...used methylation-specific PCR to examine the methylation pattern of the ...
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Original Article Semaphorin-3F functions as a tumor suppressor in colorectal cancer due to regulation by DNA methylation
... The methylation status of CpG island of all sam- ples was initially screened at SEMA3F gene pro- moter regions by methylation-specific PCR (MSP) as described ...to PCR ampli- fication ...
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KLK10 exon 3 unmethylated PCR product concentration: a new potential early diagnostic marker in ovarian cancer? - A pilot study
... Qualitative methylation-specific PCR (MSP) results did not show statistically significant differences in patient group samples (normal and tumor) where all samples were positive only for the ...
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DNA methylation: a marker for carcinogen exposure and cancer risk
... aberrant methylation was present, even in non-cancerous gastric ...titative methylation-specific PCR (qMSP) revealed that Helicobacter pylori infection potently induced aberrant DNA ...
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... DNA methylation. The success of DNA methylation however, depends on the successful conversion of the unmethylated groups and the yield of modified ...the methylation analysis of multiple genes from ...
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Promoter Methylation and Gene Expression in Human CD34+ Stem Cells Derived Erythroid Lineage by MicroRNA
... promoter methylation of the above mentioned factors does not largely mediate their upregulation during miR-451 mediated erythroid differentiation or at least the changes are such tiny which is not detected by ...
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The epigenetic modifier CHD5 functions as a novel tumor suppressor for renal cell carcinoma and is predominantly inactivated by promoter CpG methylation
... CpG methylation was involved in silencing CHD5 in urological ...cancers. Methylation-specific PCR (MSP) analysis showed that CHD5 was frequently methylated in RCC, prostate and bladder tumor ...
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“CPG Methylation in Exon 1 of TCF4 Gene as an Early Biomarker of Gastric Cancer” by Rabia Farooq, Haamid Bashir, Shajrul Amin, Bashir Ahmad Ganai, Hilal Wani, Arif Bhat, Akbar Masood, Sabhiya Majid, India.
... DNA methylation as one of the epigenetic changes and involves addition of a methyl group to the carbon 5 position of the cytosine ...the methylation specific PCR (MSP) assay that allows for ...
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Original Article The prognostic value of O 6-methylguanine-DNA methyltransferase gene promoter methylation detected by gel-based methylation-specific polymerase chain reaction in patients with glioblastoma multiforme: a systematic review
... for methylation of promoter that is more closely associated with silencing of the MGMT gene ...[10]. Methylation of the MGMT promoter, as mea- sured by MS-PCR analysis, was first confirmed to have ...
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Prediction of smoking by multiplex bisulfite PCR with long amplicons considering allele-specific effects on DNA methylation
... obtaining methylation signal from multiple targeted genome loca- tions, is the bisulfite padlock probe method ...ensure specific binding, but nonspecific binding remains problematic ...
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Therapeutic effect of daphnetin on the autoimmune arthritis through demethylation of proapoptotic genes in synovial cells
... DNA Methylation-Gold™ Kit (ZYMO RESEARCH, American). Methylation and demethylation primers were designed with the online Methprimer software (Table 1) and then synthesized by the Shanghai ShengGong bio- ...
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